Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue
Background Adipose tissue-derived stem cells (ASCs) represent a rapidly evolving area of cell-based therapies. ASCs are harvested from the stromal vascular fraction (SVF), a heterogeneous mixture of ASCs and other cell types. Two methods are typically employed to obtain the SVF: enzymatic and mechan...
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| Format: | Article |
| Language: | English |
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Korean Society for Aesthetic Plastic Surgery
2024-01-01
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| Series: | Archives of Aesthetic Plastic Surgery |
| Subjects: | |
| Online Access: | http://e-aaps.org/upload/pdf/aaps-2023-00913.pdf |
| _version_ | 1797311452169633792 |
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| author | Syeo Young Wee Jeong Do Park Sang Gue Kang Eun Soo Park |
| author_facet | Syeo Young Wee Jeong Do Park Sang Gue Kang Eun Soo Park |
| author_sort | Syeo Young Wee |
| collection | DOAJ |
| description | Background Adipose tissue-derived stem cells (ASCs) represent a rapidly evolving area of cell-based therapies. ASCs are harvested from the stromal vascular fraction (SVF), a heterogeneous mixture of ASCs and other cell types. Two methods are typically employed to obtain the SVF: enzymatic and mechanical. In this study, we examined the efficacy of ultrasonic cavitation in isolating the SVF from adipose tissue. Methods Human adipose tissue was procured from three patients through an aesthetic liposuction procedure. This tissue was then subjected to either an enzymatic method utilizing type II collagenase or an ultrasonic cavitation method using an Ultra Stemcell device. The cell counts and viability were determined using a cell counter. The immunophenotype of the SVF was analyzed using real-time polymerase chain reaction. Results Compared to the enzymatic method, the total cell count and cell viability of the SVF isolated through ultrasonic cavitation were relatively low. However, no significant difference was observed in the immunophenotype of CD45, CD11b, CD34, and CD105. Conclusions The ultrasonic cavitation method constitutes a clinically practical approach that offers straightforward access in the operative field. This technique is a rapid and cost-effective method for isolating the SVF. |
| first_indexed | 2024-03-08T01:58:54Z |
| format | Article |
| id | doaj.art-7f523c8fa8d94ddea053d457aaa7d9b8 |
| institution | Directory Open Access Journal |
| issn | 2234-0831 2288-9337 |
| language | English |
| last_indexed | 2024-03-08T01:58:54Z |
| publishDate | 2024-01-01 |
| publisher | Korean Society for Aesthetic Plastic Surgery |
| record_format | Article |
| series | Archives of Aesthetic Plastic Surgery |
| spelling | doaj.art-7f523c8fa8d94ddea053d457aaa7d9b82024-02-14T06:39:37ZengKorean Society for Aesthetic Plastic SurgeryArchives of Aesthetic Plastic Surgery2234-08312288-93372024-01-01301162110.14730/aaps.2023.00913831Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissueSyeo Young Wee0Jeong Do Park1Sang Gue Kang2Eun Soo Park3 Department of Plastic and Reconstructive Surgery, Soonchunhyang University Gumi Hospital, Gumi, Korea Department of Plastic and Reconstructive Surgery, Soonchunhyang University Gumi Hospital, Gumi, Korea Department of Plastic and Reconstructive Surgery, Soonchunhyang University Seoul Hospital, Seoul, Korea Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Bucheon, KoreaBackground Adipose tissue-derived stem cells (ASCs) represent a rapidly evolving area of cell-based therapies. ASCs are harvested from the stromal vascular fraction (SVF), a heterogeneous mixture of ASCs and other cell types. Two methods are typically employed to obtain the SVF: enzymatic and mechanical. In this study, we examined the efficacy of ultrasonic cavitation in isolating the SVF from adipose tissue. Methods Human adipose tissue was procured from three patients through an aesthetic liposuction procedure. This tissue was then subjected to either an enzymatic method utilizing type II collagenase or an ultrasonic cavitation method using an Ultra Stemcell device. The cell counts and viability were determined using a cell counter. The immunophenotype of the SVF was analyzed using real-time polymerase chain reaction. Results Compared to the enzymatic method, the total cell count and cell viability of the SVF isolated through ultrasonic cavitation were relatively low. However, no significant difference was observed in the immunophenotype of CD45, CD11b, CD34, and CD105. Conclusions The ultrasonic cavitation method constitutes a clinically practical approach that offers straightforward access in the operative field. This technique is a rapid and cost-effective method for isolating the SVF.http://e-aaps.org/upload/pdf/aaps-2023-00913.pdfstromal vascular fractionultrasonicscell survivalimmunophenotyping |
| spellingShingle | Syeo Young Wee Jeong Do Park Sang Gue Kang Eun Soo Park Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue Archives of Aesthetic Plastic Surgery stromal vascular fraction ultrasonics cell survival immunophenotyping |
| title | Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| title_full | Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| title_fullStr | Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| title_full_unstemmed | Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| title_short | Efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| title_sort | efficacy of ultrasonic cavitation in isolation of the stromal vascular fraction from adipose tissue |
| topic | stromal vascular fraction ultrasonics cell survival immunophenotyping |
| url | http://e-aaps.org/upload/pdf/aaps-2023-00913.pdf |
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