Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal
A total of 278 Ixodes ticks, collected from Madeira Island and Setúbal District, mainland Portugal, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Six (4%) of 142 Ixodes ricinus nymphs collected in Madeira Island, and 1 nymph and 1 male (2%) of 93 I....
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2004-09-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/10/9/04-0276_article |
| _version_ | 1819205385813229568 |
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| author | Ana Sofia Santos Maria Margarida Santos-Silva Victor Carlos Almeida Fátima Bacellar John Stephen Dumler |
| author_facet | Ana Sofia Santos Maria Margarida Santos-Silva Victor Carlos Almeida Fátima Bacellar John Stephen Dumler |
| author_sort | Ana Sofia Santos |
| collection | DOAJ |
| description | A total of 278 Ixodes ticks, collected from Madeira Island and Setúbal District, mainland Portugal, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Six (4%) of 142 Ixodes ricinus nymphs collected in Madeira Island, and 1 nymph and 1 male (2%) of 93 I. ventalloi collected in Setúbal District tested positive for A. phagocytophilum msp2 genes or rrs. Infection was not detected among 43 I. ricinus on mainland Portugal. All PCR products were confirmed by nucleotide sequencing to be identical or to be most closely related to A. phagocytophilum. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Setúbal District, mainland Portugal, and the first documentation of Anaplasma infection in I. ventalloi. Moreover, these findings confirm the persistence of A. phagocytophilum in Madeira Island’s I. ricinus. |
| first_indexed | 2024-12-23T04:50:52Z |
| format | Article |
| id | doaj.art-7f52df564c4943309b0de2288859ec9b |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-23T04:50:52Z |
| publishDate | 2004-09-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-7f52df564c4943309b0de2288859ec9b2022-12-21T17:59:27ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592004-09-011091643164810.3201/eid1009.040276Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland PortugalAna Sofia SantosMaria Margarida Santos-SilvaVictor Carlos AlmeidaFátima BacellarJohn Stephen DumlerA total of 278 Ixodes ticks, collected from Madeira Island and Setúbal District, mainland Portugal, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Six (4%) of 142 Ixodes ricinus nymphs collected in Madeira Island, and 1 nymph and 1 male (2%) of 93 I. ventalloi collected in Setúbal District tested positive for A. phagocytophilum msp2 genes or rrs. Infection was not detected among 43 I. ricinus on mainland Portugal. All PCR products were confirmed by nucleotide sequencing to be identical or to be most closely related to A. phagocytophilum. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Setúbal District, mainland Portugal, and the first documentation of Anaplasma infection in I. ventalloi. Moreover, these findings confirm the persistence of A. phagocytophilum in Madeira Island’s I. ricinus.https://wwwnc.cdc.gov/eid/article/10/9/04-0276_articlePortugalAnaplasma phagocytophilumPolymerase chain reaction (PCR)TicksIxodes ricinusIxodes ventalloi |
| spellingShingle | Ana Sofia Santos Maria Margarida Santos-Silva Victor Carlos Almeida Fátima Bacellar John Stephen Dumler Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal Emerging Infectious Diseases Portugal Anaplasma phagocytophilum Polymerase chain reaction (PCR) Ticks Ixodes ricinus Ixodes ventalloi |
| title | Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal |
| title_full | Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal |
| title_fullStr | Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal |
| title_full_unstemmed | Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal |
| title_short | Detection of Anaplasma phagocytophilum DNA in Ixodes Ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, Mainland Portugal |
| title_sort | detection of anaplasma phagocytophilum dna in ixodes ticks acari ixodidae from madeira island and setubal district mainland portugal |
| topic | Portugal Anaplasma phagocytophilum Polymerase chain reaction (PCR) Ticks Ixodes ricinus Ixodes ventalloi |
| url | https://wwwnc.cdc.gov/eid/article/10/9/04-0276_article |
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