Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes.
In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomy...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4556377?pdf=render |
| _version_ | 1818791292258222080 |
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| author | Laura Tarnawski Xiaojie Xian Gustavo Monnerat Iain C Macaulay Daniela Malan Andrew Borgman Sean M Wu Bernd K Fleischmann Stefan Jovinge |
| author_facet | Laura Tarnawski Xiaojie Xian Gustavo Monnerat Iain C Macaulay Daniela Malan Andrew Borgman Sean M Wu Bernd K Fleischmann Stefan Jovinge |
| author_sort | Laura Tarnawski |
| collection | DOAJ |
| description | In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellular markers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surface marker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes. |
| first_indexed | 2024-12-18T15:09:02Z |
| format | Article |
| id | doaj.art-7f544021333c48dd93c441c2c22e1878 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T15:09:02Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7f544021333c48dd93c441c2c22e18782022-12-21T21:03:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013588010.1371/journal.pone.0135880Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes.Laura TarnawskiXiaojie XianGustavo MonneratIain C MacaulayDaniela MalanAndrew BorgmanSean M WuBernd K FleischmannStefan JovingeIn contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellular markers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surface marker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes.http://europepmc.org/articles/PMC4556377?pdf=render |
| spellingShingle | Laura Tarnawski Xiaojie Xian Gustavo Monnerat Iain C Macaulay Daniela Malan Andrew Borgman Sean M Wu Bernd K Fleischmann Stefan Jovinge Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. PLoS ONE |
| title | Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. |
| title_full | Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. |
| title_fullStr | Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. |
| title_full_unstemmed | Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. |
| title_short | Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes. |
| title_sort | integrin based isolation enables purification of murine lineage committed cardiomyocytes |
| url | http://europepmc.org/articles/PMC4556377?pdf=render |
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