Tracking mesenchymal stem cells using magnetic resonance imaging
Recent translational studies in the fields of tissue regeneration and cell therapy have characterized mesenchymal stem cells (MSCs) as a potentially effective and accessible measure for treating ischemic cerebral and neurodegenerative disorders such as stroke, Parkinson's disease, and amyotroph...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Brain Circulation |
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| Online Access: | http://www.braincirculation.org/article.asp?issn=2394-8108;year=2016;volume=2;issue=3;spage=108;epage=113;aulast=Rosenberg |
| _version_ | 1811334884306714624 |
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| author | Jens T Rosenberg Xuegang Yuan Samuel Grant Teng Ma |
| author_facet | Jens T Rosenberg Xuegang Yuan Samuel Grant Teng Ma |
| author_sort | Jens T Rosenberg |
| collection | DOAJ |
| description | Recent translational studies in the fields of tissue regeneration and cell therapy have characterized mesenchymal stem cells (MSCs) as a potentially effective and accessible measure for treating ischemic cerebral and neurodegenerative disorders such as stroke, Parkinson's disease, and amyotrophic lateral sclerosis. Developing more efficient cell tracking techniques bear the potential to optimize MSC transplantation therapies by providing a more accurate picture of the fate and area of effect of implanted cells. Currently, determining the location of transplanted MSCs involves a histological approach, but magnetic resonance imaging (MRI) presents a noninvasive paradigm that permits repeat evaluations. To visualize MSCs using MRI, the implanted cells must be treated with an intracellular contrast agent. These are commonly paramagnetic compounds, many of which are based on superparamagnetic iron oxide (SPIO) nanoparticles. Recent research has set out characterize the effects of SPIO-uptake on the cellular activity of in vitro human MSCs and the resultant influence that respective SPIO concentration has on MRI sensitivity. As these studies reveal, SPIO-uptake has no effect on the cellular processes of proliferation and differentiation while producing high contrast MRI signals. Moreover, transplantation of SPIO-labeled MSCs in animal models encouragingly showed no loss in MRI contrast, suggesting that SPIO labeling may be an appealing regime for lasting MRI detection. This study is a review article. Referred literature in this study has been listed in the reference part. The datasets supporting the conclusions of this article are available online by searching the PubMed. Some original points in this article come from the laboratory practice in our research centers and the authors' experiences. |
| first_indexed | 2024-04-13T17:15:49Z |
| format | Article |
| id | doaj.art-7f5473622a2244898f096012e56fef38 |
| institution | Directory Open Access Journal |
| issn | 2455-4626 |
| language | English |
| last_indexed | 2024-04-13T17:15:49Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Brain Circulation |
| spelling | doaj.art-7f5473622a2244898f096012e56fef382022-12-22T02:38:08ZengWolters Kluwer Medknow PublicationsBrain Circulation2455-46262016-01-012310811310.4103/2394-8108.192521Tracking mesenchymal stem cells using magnetic resonance imagingJens T RosenbergXuegang YuanSamuel GrantTeng MaRecent translational studies in the fields of tissue regeneration and cell therapy have characterized mesenchymal stem cells (MSCs) as a potentially effective and accessible measure for treating ischemic cerebral and neurodegenerative disorders such as stroke, Parkinson's disease, and amyotrophic lateral sclerosis. Developing more efficient cell tracking techniques bear the potential to optimize MSC transplantation therapies by providing a more accurate picture of the fate and area of effect of implanted cells. Currently, determining the location of transplanted MSCs involves a histological approach, but magnetic resonance imaging (MRI) presents a noninvasive paradigm that permits repeat evaluations. To visualize MSCs using MRI, the implanted cells must be treated with an intracellular contrast agent. These are commonly paramagnetic compounds, many of which are based on superparamagnetic iron oxide (SPIO) nanoparticles. Recent research has set out characterize the effects of SPIO-uptake on the cellular activity of in vitro human MSCs and the resultant influence that respective SPIO concentration has on MRI sensitivity. As these studies reveal, SPIO-uptake has no effect on the cellular processes of proliferation and differentiation while producing high contrast MRI signals. Moreover, transplantation of SPIO-labeled MSCs in animal models encouragingly showed no loss in MRI contrast, suggesting that SPIO labeling may be an appealing regime for lasting MRI detection. This study is a review article. Referred literature in this study has been listed in the reference part. The datasets supporting the conclusions of this article are available online by searching the PubMed. Some original points in this article come from the laboratory practice in our research centers and the authors' experiences.http://www.braincirculation.org/article.asp?issn=2394-8108;year=2016;volume=2;issue=3;spage=108;epage=113;aulast=RosenbergCell trackinghuman mesenchymal stem cellshypoxiaischemiamagnetic resonance imagingsuperparamagnetic iron oxide |
| spellingShingle | Jens T Rosenberg Xuegang Yuan Samuel Grant Teng Ma Tracking mesenchymal stem cells using magnetic resonance imaging Brain Circulation Cell tracking human mesenchymal stem cells hypoxia ischemia magnetic resonance imaging superparamagnetic iron oxide |
| title | Tracking mesenchymal stem cells using magnetic resonance imaging |
| title_full | Tracking mesenchymal stem cells using magnetic resonance imaging |
| title_fullStr | Tracking mesenchymal stem cells using magnetic resonance imaging |
| title_full_unstemmed | Tracking mesenchymal stem cells using magnetic resonance imaging |
| title_short | Tracking mesenchymal stem cells using magnetic resonance imaging |
| title_sort | tracking mesenchymal stem cells using magnetic resonance imaging |
| topic | Cell tracking human mesenchymal stem cells hypoxia ischemia magnetic resonance imaging superparamagnetic iron oxide |
| url | http://www.braincirculation.org/article.asp?issn=2394-8108;year=2016;volume=2;issue=3;spage=108;epage=113;aulast=Rosenberg |
| work_keys_str_mv | AT jenstrosenberg trackingmesenchymalstemcellsusingmagneticresonanceimaging AT xuegangyuan trackingmesenchymalstemcellsusingmagneticresonanceimaging AT samuelgrant trackingmesenchymalstemcellsusingmagneticresonanceimaging AT tengma trackingmesenchymalstemcellsusingmagneticresonanceimaging |