Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens
Abstract Background Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and est...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07679-y |
| _version_ | 1819099774239899648 |
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| author | Junxiao Ren Weihua Tian Keren Jiang Zhang Wang Dandan Wang Zhuanjian Li Fengbin Yan Yanbin Wang Yadong Tian Kepeng Ou Hongjun Wang Xiangtao Kang Hong Li Xiaojun Liu |
| author_facet | Junxiao Ren Weihua Tian Keren Jiang Zhang Wang Dandan Wang Zhuanjian Li Fengbin Yan Yanbin Wang Yadong Tian Kepeng Ou Hongjun Wang Xiangtao Kang Hong Li Xiaojun Liu |
| author_sort | Junxiao Ren |
| collection | DOAJ |
| description | Abstract Background Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and estrogen functional sites on a genome-wide scale, we determined expression profiles of mRNAs, lncRNAs, and miRNAs in estrogen-treated ((17β-estradiol)) and control chicken livers using RNA-Sequencing (RNA-Seq) and studied the estrogen receptor α binding sites by ChIP-Sequencing (ChIP-Seq). Results We identified a total of 990 estrogen-responsive genes, including 962 protein-coding genes, 11 miRNAs, and 17 lncRNAs. Functional enrichment analyses showed that the estrogen-responsive genes were highly enriched in lipid metabolism and biological processes. Integrated analysis of the data of RNA-Seq and ChIP-Seq, identified 191 genes directly targeted by estrogen, including 185 protein-coding genes, 4 miRNAs, and 2 lncRNAs. In vivo and in vitro experiments showed that estrogen decreased the mRNA expression of PPARGC1B, which had been reported to be linked with lipid metabolism, by directly increasing the expression of miR-144-3p. Conclusions These results increase our understanding of the functional network of estrogen in chicken liver and also reveal aspects of the molecular mechanism of estrogen-related lipid metabolism. |
| first_indexed | 2024-12-22T00:52:13Z |
| format | Article |
| id | doaj.art-7f56e3ac64f04c85ac6f8cd10d4781d8 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T00:52:13Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-7f56e3ac64f04c85ac6f8cd10d4781d82022-12-21T18:44:25ZengBMCBMC Genomics1471-21642021-06-0122111410.1186/s12864-021-07679-yGlobal investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hensJunxiao Ren0Weihua Tian1Keren Jiang2Zhang Wang3Dandan Wang4Zhuanjian Li5Fengbin Yan6Yanbin Wang7Yadong Tian8Kepeng Ou9Hongjun Wang10Xiangtao Kang11Hong Li12Xiaojun Liu13College of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Pharmacy, National and Local Joint Engineering Research Center of Targeted and Innovative Therapeutics, Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, Chongqing University of Arts and SciencesCenter for Cellular Therapy, Medical University of South CarolinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityAbstract Background Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and estrogen functional sites on a genome-wide scale, we determined expression profiles of mRNAs, lncRNAs, and miRNAs in estrogen-treated ((17β-estradiol)) and control chicken livers using RNA-Sequencing (RNA-Seq) and studied the estrogen receptor α binding sites by ChIP-Sequencing (ChIP-Seq). Results We identified a total of 990 estrogen-responsive genes, including 962 protein-coding genes, 11 miRNAs, and 17 lncRNAs. Functional enrichment analyses showed that the estrogen-responsive genes were highly enriched in lipid metabolism and biological processes. Integrated analysis of the data of RNA-Seq and ChIP-Seq, identified 191 genes directly targeted by estrogen, including 185 protein-coding genes, 4 miRNAs, and 2 lncRNAs. In vivo and in vitro experiments showed that estrogen decreased the mRNA expression of PPARGC1B, which had been reported to be linked with lipid metabolism, by directly increasing the expression of miR-144-3p. Conclusions These results increase our understanding of the functional network of estrogen in chicken liver and also reveal aspects of the molecular mechanism of estrogen-related lipid metabolism.https://doi.org/10.1186/s12864-021-07679-yRNA-SeqChIP-SeqmiR-144, estrogenChickenLiver |
| spellingShingle | Junxiao Ren Weihua Tian Keren Jiang Zhang Wang Dandan Wang Zhuanjian Li Fengbin Yan Yanbin Wang Yadong Tian Kepeng Ou Hongjun Wang Xiangtao Kang Hong Li Xiaojun Liu Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens BMC Genomics RNA-Seq ChIP-Seq miR-144, estrogen Chicken Liver |
| title | Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens |
| title_full | Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens |
| title_fullStr | Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens |
| title_full_unstemmed | Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens |
| title_short | Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens |
| title_sort | global investigation of estrogen responsive genes regulating lipid metabolism in the liver of laying hens |
| topic | RNA-Seq ChIP-Seq miR-144, estrogen Chicken Liver |
| url | https://doi.org/10.1186/s12864-021-07679-y |
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