Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions
Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired products from low-cost substrates. Here, a cell-free synthetic bios...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-09-01
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| Series: | Synthetic and Systems Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X18300346 |
| _version_ | 1797203577041584128 |
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| author | Leipeng Xie Xinlei Wei Xigui Zhou Dongdong Meng Ruimin Zhou Yi-Heng P. Job Zhang Shuxia Xu Chun You |
| author_facet | Leipeng Xie Xinlei Wei Xigui Zhou Dongdong Meng Ruimin Zhou Yi-Heng P. Job Zhang Shuxia Xu Chun You |
| author_sort | Leipeng Xie |
| collection | DOAJ |
| description | Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired products from low-cost substrates. Here, a cell-free synthetic biosystem containing minimized number of reactions was designed for the conversion of d-glucose to l-lactate via pyruvate. This NADH-balanced biosystem was comprised of only 5 thermophilic enzymes without ATP supplementation. After optimization of enzyme loading amounts, buffer concentration and cofactor concentration, d-glucose was converted to l-lactate with a product yield of ∼90%. Our study has provided an emerging platform with potentials in producing pyruvate-derived chemicals, and may promote the development of cell-free synthetic enzymatic biosystems for biomanufacturing. Keywords: Cell-free synthetic enzymatic biosystem, Minimized reactions, Thermophilic enzymes, l-lactate, Pyruvate, Biomanufacturing |
| first_indexed | 2024-04-24T08:21:32Z |
| format | Article |
| id | doaj.art-7f57a315483146cd9b9b9f4d1d00ddf4 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:21:32Z |
| publishDate | 2018-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-7f57a315483146cd9b9b9f4d1d00ddf42024-04-17T00:45:47ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2018-09-0133204210Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactionsLeipeng Xie0Xinlei Wei1Xigui Zhou2Dongdong Meng3Ruimin Zhou4Yi-Heng P. Job Zhang5Shuxia Xu6Chun You7College of Life Sciences, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, ChinaCollege of Life Sciences, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China; Corresponding author.Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China; Corresponding author.Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired products from low-cost substrates. Here, a cell-free synthetic biosystem containing minimized number of reactions was designed for the conversion of d-glucose to l-lactate via pyruvate. This NADH-balanced biosystem was comprised of only 5 thermophilic enzymes without ATP supplementation. After optimization of enzyme loading amounts, buffer concentration and cofactor concentration, d-glucose was converted to l-lactate with a product yield of ∼90%. Our study has provided an emerging platform with potentials in producing pyruvate-derived chemicals, and may promote the development of cell-free synthetic enzymatic biosystems for biomanufacturing. Keywords: Cell-free synthetic enzymatic biosystem, Minimized reactions, Thermophilic enzymes, l-lactate, Pyruvate, Biomanufacturinghttp://www.sciencedirect.com/science/article/pii/S2405805X18300346 |
| spellingShingle | Leipeng Xie Xinlei Wei Xigui Zhou Dongdong Meng Ruimin Zhou Yi-Heng P. Job Zhang Shuxia Xu Chun You Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions Synthetic and Systems Biotechnology |
| title | Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions |
| title_full | Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions |
| title_fullStr | Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions |
| title_full_unstemmed | Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions |
| title_short | Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions |
| title_sort | conversion of d glucose to l lactate via pyruvate by an optimized cell free enzymatic biosystem containing minimized reactions |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X18300346 |
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