New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice
Sheath blight disease caused by the necrotrophic, soil-borne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 ri...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2022-12-01
|
| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2022-0054.pdf |
| _version_ | 1811177537213038592 |
|---|---|
| author | Pachai Poonguzhali Ashish Chauhan Abinash Kar Shivaji Lavale Spurthi N. Nayak S. K. Prashanthi |
| author_facet | Pachai Poonguzhali Ashish Chauhan Abinash Kar Shivaji Lavale Spurthi N. Nayak S. K. Prashanthi |
| author_sort | Pachai Poonguzhali |
| collection | DOAJ |
| description | Sheath blight disease caused by the necrotrophic, soil-borne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 rice landraces were screened against R. solani under artificial epiphytotics and identified six moderately resistant landraces, Jigguvaratiga, Honasu, Jeer Sali, Jeeraga-2, BiliKagga, and Medini Sannabatta with relative lesion height (RLH) range of 21–30%. Landrace Jigguvaratiga with consistent and better level of resistance (21% RLH) than resistant check Tetep (RLH 28%) was used to develop mapping population. DNA markers associated with ShB resistance were identified in F2 mapping population developed from Jigguvaratiga × BPT5204 (susceptible variety) using bulk segregant analysis. Among 56 parental polymorphic markers, RM5556, RM6208, and RM7 were polymorphic between the bulks. Single marker analysis indicated the significant association of ShB with RM5556 and RM6208 with phenotypic variance (R2) of 28.29 and 20.06%, respectively. Co-segregation analysis confirmed the strong association of RM5556 and RM6208 located on chromosome 8 for ShB trait. This is the first report on association of RM6208 marker for ShB resistance. In silico analysis revealed that RM6208 loci resides the stearoyl ACP desaturases protein, which is involved in defense mechanism against plant pathogens. RM5556 loci resides a protein, with unknown function. The putative candidate genes or quantitative trait locus harbouring at the marker interval of RM5556 and RM6208 can be further used to develop ShB resistant varieties using molecular breeding approaches. |
| first_indexed | 2024-04-11T06:03:08Z |
| format | Article |
| id | doaj.art-7f58b5aa0cec4831876d9777bc6d1312 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-11T06:03:08Z |
| publishDate | 2022-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-7f58b5aa0cec4831876d9777bc6d13122022-12-22T04:41:35ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802022-12-0138657258210.5423/PPJ.OA.04.2022.00542352New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in RicePachai Poonguzhali0Ashish Chauhan1Abinash Kar2Shivaji Lavale3Spurthi N. Nayak4S. K. Prashanthi5 Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, India Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, India Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, India Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, India Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, India Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, Karnataka, IndiaSheath blight disease caused by the necrotrophic, soil-borne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 rice landraces were screened against R. solani under artificial epiphytotics and identified six moderately resistant landraces, Jigguvaratiga, Honasu, Jeer Sali, Jeeraga-2, BiliKagga, and Medini Sannabatta with relative lesion height (RLH) range of 21–30%. Landrace Jigguvaratiga with consistent and better level of resistance (21% RLH) than resistant check Tetep (RLH 28%) was used to develop mapping population. DNA markers associated with ShB resistance were identified in F2 mapping population developed from Jigguvaratiga × BPT5204 (susceptible variety) using bulk segregant analysis. Among 56 parental polymorphic markers, RM5556, RM6208, and RM7 were polymorphic between the bulks. Single marker analysis indicated the significant association of ShB with RM5556 and RM6208 with phenotypic variance (R2) of 28.29 and 20.06%, respectively. Co-segregation analysis confirmed the strong association of RM5556 and RM6208 located on chromosome 8 for ShB trait. This is the first report on association of RM6208 marker for ShB resistance. In silico analysis revealed that RM6208 loci resides the stearoyl ACP desaturases protein, which is involved in defense mechanism against plant pathogens. RM5556 loci resides a protein, with unknown function. The putative candidate genes or quantitative trait locus harbouring at the marker interval of RM5556 and RM6208 can be further used to develop ShB resistant varieties using molecular breeding approaches.http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2022-0054.pdfbulk segregant analysisdna markersresistancericesheath blight |
| spellingShingle | Pachai Poonguzhali Ashish Chauhan Abinash Kar Shivaji Lavale Spurthi N. Nayak S. K. Prashanthi New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice The Plant Pathology Journal bulk segregant analysis dna markers resistance rice sheath blight |
| title | New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice |
| title_full | New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice |
| title_fullStr | New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice |
| title_full_unstemmed | New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice |
| title_short | New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice |
| title_sort | new sources of resistance and identification of dna marker loci for sheath blight disease caused by rhizoctonia solani kuhn in rice |
| topic | bulk segregant analysis dna markers resistance rice sheath blight |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-04-2022-0054.pdf |
| work_keys_str_mv | AT pachaipoonguzhali newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice AT ashishchauhan newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice AT abinashkar newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice AT shivajilavale newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice AT spurthinnayak newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice AT skprashanthi newsourcesofresistanceandidentificationofdnamarkerlociforsheathblightdiseasecausedbyrhizoctoniasolanikuhninrice |