Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS

Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS

A high performance liquid chromatography tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) protocol was developed for the determination of diphenylamine (DPA). Four productions of DPA were selected for qualitative assay and the peak area of the main product ion for quantitation. By means of se...

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Bibliographic Details
Main Authors: Hongcheng Mei, Yangke Quan, Wenhao Wang, Hong Zhou, Zhanfang Liu, Huixia Shi, Peng Wang
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:Journal of Forensic Science and Medicine
Subjects:
Diphenylamine
gunshot residue
high performance liquid chromatography-mass spectrometry/mass spectrometry
Online Access:http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=1;spage=18;epage=21;aulast=Mei
Description
Summary:A high performance liquid chromatography tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) protocol was developed for the determination of diphenylamine (DPA). Four productions of DPA were selected for qualitative assay and the peak area of the main product ion for quantitation. By means of separation using an Agilent Extend-C18 column (CA, USA) (150 mm × 4.6 mm, 5 μm) with methanol-water (90:10) as the mobile phase, DPA was detected by electrospray ionization (ESI) tandem mass spectrometry in positive mode. The linearity of the peak area versus concentration ranged 5-500 ng/mL, r 2 = 0.9978. The limit of detection (S/N =3) of this method was 0.3 ng/mL. This method is applicable for the determination of DPA in gunshot residue.
ISSN:2349-5014

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