Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS
A high performance liquid chromatography tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) protocol was developed for the determination of diphenylamine (DPA). Four productions of DPA were selected for qualitative assay and the peak area of the main product ion for quantitation. By means of se...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Forensic Science and Medicine |
| Subjects: | |
| Online Access: | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=1;spage=18;epage=21;aulast=Mei |
| _version_ | 1819043374838054912 |
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| author | Hongcheng Mei Yangke Quan Wenhao Wang Hong Zhou Zhanfang Liu Huixia Shi Peng Wang |
| author_facet | Hongcheng Mei Yangke Quan Wenhao Wang Hong Zhou Zhanfang Liu Huixia Shi Peng Wang |
| author_sort | Hongcheng Mei |
| collection | DOAJ |
| description | A high performance liquid chromatography tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) protocol was developed for the determination of diphenylamine (DPA). Four productions of DPA were selected for qualitative assay and the peak area of the main product ion for quantitation. By means of separation using an Agilent Extend-C18 column (CA, USA) (150 mm × 4.6 mm, 5 μm) with methanol-water (90:10) as the mobile phase, DPA was detected by electrospray ionization (ESI) tandem mass spectrometry in positive mode. The linearity of the peak area versus concentration ranged 5-500 ng/mL, r 2 = 0.9978. The limit of detection (S/N =3) of this method was 0.3 ng/mL. This method is applicable for the determination of DPA in gunshot residue. |
| first_indexed | 2024-12-21T09:55:46Z |
| format | Article |
| id | doaj.art-7f59f99a18f0496ab2eea6c6dbf06f5f |
| institution | Directory Open Access Journal |
| issn | 2349-5014 |
| language | English |
| last_indexed | 2024-12-21T09:55:46Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Forensic Science and Medicine |
| spelling | doaj.art-7f59f99a18f0496ab2eea6c6dbf06f5f2022-12-21T19:08:05ZengWolters Kluwer Medknow PublicationsJournal of Forensic Science and Medicine2349-50142016-01-0121182110.4103/2349-5014.162808Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MSHongcheng MeiYangke QuanWenhao WangHong ZhouZhanfang LiuHuixia ShiPeng WangA high performance liquid chromatography tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) protocol was developed for the determination of diphenylamine (DPA). Four productions of DPA were selected for qualitative assay and the peak area of the main product ion for quantitation. By means of separation using an Agilent Extend-C18 column (CA, USA) (150 mm × 4.6 mm, 5 μm) with methanol-water (90:10) as the mobile phase, DPA was detected by electrospray ionization (ESI) tandem mass spectrometry in positive mode. The linearity of the peak area versus concentration ranged 5-500 ng/mL, r 2 = 0.9978. The limit of detection (S/N =3) of this method was 0.3 ng/mL. This method is applicable for the determination of DPA in gunshot residue.http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=1;spage=18;epage=21;aulast=MeiDiphenylaminegunshot residuehigh performance liquid chromatography-mass spectrometry/mass spectrometry |
| spellingShingle | Hongcheng Mei Yangke Quan Wenhao Wang Hong Zhou Zhanfang Liu Huixia Shi Peng Wang Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS Journal of Forensic Science and Medicine Diphenylamine gunshot residue high performance liquid chromatography-mass spectrometry/mass spectrometry |
| title | Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS |
| title_full | Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS |
| title_fullStr | Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS |
| title_full_unstemmed | Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS |
| title_short | Determination of Diphenylamine in Gunshot Residue by HPLC-MS/MS |
| title_sort | determination of diphenylamine in gunshot residue by hplc ms ms |
| topic | Diphenylamine gunshot residue high performance liquid chromatography-mass spectrometry/mass spectrometry |
| url | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=1;spage=18;epage=21;aulast=Mei |
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