Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2285 |
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| author | Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha |
| author_facet | Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha |
| author_sort | Rafaela Perdigão |
| collection | DOAJ |
| description | This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation. |
| first_indexed | 2024-03-10T05:15:54Z |
| format | Article |
| id | doaj.art-7f5d1210f99d4e339e295e50e458285e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:54Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7f5d1210f99d4e339e295e50e458285e2023-11-23T00:29:07ZengMDPI AGMicroorganisms2076-26072021-11-01911228510.3390/microorganisms9112285Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading BacteriaRafaela Perdigão0C. Marisa R. Almeida1Catarina Magalhães2Sandra Ramos3Ana L. Carolas4Bruno S. Ferreira5Maria F. Carvalho6Ana P. Mucha7CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalBiotrend S.A., Biocant Park, Núcleo 04 Lote 2, 3060-197 Cantanhede, PortugalBiotrend S.A., Biocant Park, Núcleo 04 Lote 2, 3060-197 Cantanhede, PortugalCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalCIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalThis work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.https://www.mdpi.com/2076-2607/9/11/2285autochthonous bioremediationoil spillshydrocarbonsbioaugmentationbioremediation agentlyophilized bacteria |
| spellingShingle | Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria Microorganisms autochthonous bioremediation oil spills hydrocarbons bioaugmentation bioremediation agent lyophilized bacteria |
| title | Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria |
| title_full | Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria |
| title_fullStr | Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria |
| title_full_unstemmed | Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria |
| title_short | Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria |
| title_sort | bioremediation of petroleum hydrocarbons in seawater prospects of using lyophilized native hydrocarbon degrading bacteria |
| topic | autochthonous bioremediation oil spills hydrocarbons bioaugmentation bioremediation agent lyophilized bacteria |
| url | https://www.mdpi.com/2076-2607/9/11/2285 |
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