Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis
Background: In developing countries, the most common cause of neonatal mortality is neonatal sepsis. Currently, the most significant and common hospital acquired pathogen is Escherichia coli, associated with many problems such as septicemia, pneumonia and meningitis in the neonates. Due to emerging...
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| Format: | Article |
| Language: | English |
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Jinnah University for Women
2021-12-01
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| Series: | RADS Journal of Biological Research & Applied Science |
| Subjects: | |
| Online Access: | http://jbas.juw.edu.pk/index.php/JBAS/article/view/382 |
| _version_ | 1819236910861647872 |
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| author | Javaid Akhter Hashmi Aaliya Javaid Agha Syed Ali Haider Naqvi Muhammad Umar Javiad Hashmi |
| author_facet | Javaid Akhter Hashmi Aaliya Javaid Agha Syed Ali Haider Naqvi Muhammad Umar Javiad Hashmi |
| author_sort | Javaid Akhter Hashmi |
| collection | DOAJ |
| description | Background: In developing countries, the most common cause of neonatal mortality is neonatal sepsis. Currently, the most significant and common hospital acquired pathogen is Escherichia coli, associated with many problems such as septicemia, pneumonia and meningitis in the neonates. Due to emerging antibiotic resistance in microorganisms, the current antibiotics which are extensively used are insufficient to resolve the issues.
Objectives: The aim of the study was to find out the frequency and antibiogram assay of Escherichia coli in neonatal sepsis.
Methodology: The research study was cross sectional study directed by the Department of Neonatology, Bahawal Victoria Hospital, Bahawalpur. The study duration for the current study was from September 2019 to January 2021. Blood samples were collected from all the suspected neonates for isolation and identification of E.coli, and standard microbiological protocols were used for the identification. Further a modified Kirby Bauer method of disk diffusion was used antibiotic sensitivity testing.
Results: During the defined study duration, total 150 blood cultures were found positive for neonatal sepsis. Prevalence of E.coli in these 150 positive blood cultures was 70% (n = 105). The most effective antibiotics observed in our study were gatifloxacin, imipenam and amikacin, while the least effective antibiotics were vancomycin, ampicillin, gentamycin and linezolid. Gatifloxacin shows 100% sensitivity against all isolates of E.coli, while all E.coli isolates show 100% resistance to vancomycin.
Conclusion: Our study concluded that E.coli is a major cause for neonatal sepsis in neonates admitted at Bahawal Victoria Hospital, Bahawalpur. The resistance pattern was alarmingly increased as observed in the currently available antibiotics. Therefore, surveillance of this emerging resistance is needed in these antibiotics. Furthermore, in order to limit the resistant strains of the pathogens, there is a need of effective infections control program. |
| first_indexed | 2024-12-23T13:11:57Z |
| format | Article |
| id | doaj.art-7f5f7a94b0e144bea81130a981bd94cc |
| institution | Directory Open Access Journal |
| issn | 2305-8722 2521-8573 |
| language | English |
| last_indexed | 2024-12-23T13:11:57Z |
| publishDate | 2021-12-01 |
| publisher | Jinnah University for Women |
| record_format | Article |
| series | RADS Journal of Biological Research & Applied Science |
| spelling | doaj.art-7f5f7a94b0e144bea81130a981bd94cc2022-12-21T17:45:43ZengJinnah University for WomenRADS Journal of Biological Research & Applied Science2305-87222521-85732021-12-0112210.37962/jbas.v12i2.382Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal SepsisJavaid Akhter Hashmi0Aaliya Javaid1Agha Syed Ali Haider Naqvi2Muhammad Umar Javiad Hashmi3Department of Community Medicine, Shahida Islam Medical and Dental College, Lodhran, Pakistan.Department of Gynaecology, Syed Hospital, Bahawalpur, Pakistan.Federal Medical and Dental College, Islamabad, Pakistan.HBS Medical and Dental College, Islamabad, Pakistan.Background: In developing countries, the most common cause of neonatal mortality is neonatal sepsis. Currently, the most significant and common hospital acquired pathogen is Escherichia coli, associated with many problems such as septicemia, pneumonia and meningitis in the neonates. Due to emerging antibiotic resistance in microorganisms, the current antibiotics which are extensively used are insufficient to resolve the issues. Objectives: The aim of the study was to find out the frequency and antibiogram assay of Escherichia coli in neonatal sepsis. Methodology: The research study was cross sectional study directed by the Department of Neonatology, Bahawal Victoria Hospital, Bahawalpur. The study duration for the current study was from September 2019 to January 2021. Blood samples were collected from all the suspected neonates for isolation and identification of E.coli, and standard microbiological protocols were used for the identification. Further a modified Kirby Bauer method of disk diffusion was used antibiotic sensitivity testing. Results: During the defined study duration, total 150 blood cultures were found positive for neonatal sepsis. Prevalence of E.coli in these 150 positive blood cultures was 70% (n = 105). The most effective antibiotics observed in our study were gatifloxacin, imipenam and amikacin, while the least effective antibiotics were vancomycin, ampicillin, gentamycin and linezolid. Gatifloxacin shows 100% sensitivity against all isolates of E.coli, while all E.coli isolates show 100% resistance to vancomycin. Conclusion: Our study concluded that E.coli is a major cause for neonatal sepsis in neonates admitted at Bahawal Victoria Hospital, Bahawalpur. The resistance pattern was alarmingly increased as observed in the currently available antibiotics. Therefore, surveillance of this emerging resistance is needed in these antibiotics. Furthermore, in order to limit the resistant strains of the pathogens, there is a need of effective infections control program.http://jbas.juw.edu.pk/index.php/JBAS/article/view/382Antibiotics, Escherichia coli, Frequency, Neonatal Sepsis, Susceptibility Pattern. |
| spellingShingle | Javaid Akhter Hashmi Aaliya Javaid Agha Syed Ali Haider Naqvi Muhammad Umar Javiad Hashmi Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis RADS Journal of Biological Research & Applied Science Antibiotics, Escherichia coli, Frequency, Neonatal Sepsis, Susceptibility Pattern. |
| title | Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis |
| title_full | Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis |
| title_fullStr | Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis |
| title_full_unstemmed | Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis |
| title_short | Frequency and Antibiotic Resistance Profile of Escherichia coli in Neonatal Sepsis |
| title_sort | frequency and antibiotic resistance profile of escherichia coli in neonatal sepsis |
| topic | Antibiotics, Escherichia coli, Frequency, Neonatal Sepsis, Susceptibility Pattern. |
| url | http://jbas.juw.edu.pk/index.php/JBAS/article/view/382 |
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