Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress
Abstract Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04767-5 |
| _version_ | 1797275288395055104 |
|---|---|
| author | Fazal Amin Arwa Abdulkreem AL-Huqail Sami Ullah Muhammad Nauman Khan Alevcan Kaplan Baber Ali Majid Iqbal Fahmy Gad Elsaid Sezai Ercisli Tabarak Malik Sami Asir Al-Robai Amany H. A. Abeed |
| author_facet | Fazal Amin Arwa Abdulkreem AL-Huqail Sami Ullah Muhammad Nauman Khan Alevcan Kaplan Baber Ali Majid Iqbal Fahmy Gad Elsaid Sezai Ercisli Tabarak Malik Sami Asir Al-Robai Amany H. A. Abeed |
| author_sort | Fazal Amin |
| collection | DOAJ |
| description | Abstract Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed (Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.( T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly (p > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil. |
| first_indexed | 2024-03-07T15:11:09Z |
| format | Article |
| id | doaj.art-7f5f90cb6c3a4d188af5bb93531680f5 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:11:09Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-7f5f90cb6c3a4d188af5bb93531680f52024-03-05T18:38:13ZengBMCBMC Plant Biology1471-22292024-02-0124111810.1186/s12870-024-04767-5Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stressFazal Amin0Arwa Abdulkreem AL-Huqail1Sami Ullah2Muhammad Nauman Khan3Alevcan Kaplan4Baber Ali5Majid Iqbal6Fahmy Gad Elsaid7Sezai Ercisli8Tabarak Malik9Sami Asir Al-Robai10Amany H. A. Abeed11Department of Botany, University of PeshawarDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Botany, University of PeshawarDepartment of Botany, Islamia CollegeDepartment of Crop and Animal Production, Sason Vocational School, Batman UniversityDepartment of Plant Sciences, Quaid-I-Azam UniversityInstitute of Geographic Sciences and Natural Resources Research, University of Chinese Academy of SciencesBiology Department, College of Science, King Khalid UniversityDepartment of Horticulture, Agricultural Faculty, Ataturk UniversityDepartment of Biomedical Sciences, Institute of Health, Jimma UniversityDepartment of Biology, Faculty of Science, Al-Baha UniversityDepartment of Botany and Microbiology, Faculty of Science, Assiut UniversityAbstract Soil pollution with heavy metals has grown to be a big hassle, leading to the loss in farming production particularly in developing countries like Pakistan, where no proper channel is present for irrigation and extraction of these toxic heavy metals. The present study aims to ameliorate the damages caused by heavy metal ions (Hg-Mercury) on rapeseed (Brassica napus L.) via a growth regulator (α-tocopherol 150 mg/L) and thermopriming technique at 4 °C and 50 °C to maintain plant agronomical and physiological characteristics. In pot experiments, we designed total of 11 treatments viz.( T0 (control), T1 (Hg4ppm), T2 (Hg8ppm), T3 (Hg4ppm + 4 °C), T4 (Hg4ppm + 4 °C + tocopherol (150 m/L)), T5 (Hg4ppm + 50 °C), T6 (Hg4ppm + 50 °C + tocopherol (150 mg/L)), T7 (Hg8ppm + 4 °C), T8 (Hg8ppm + 4 °C + tocopherol (150 mg/L)), T9 (Hg8ppm + 50 °C), T10 (Hg8ppm + 50 °C + tocopherol (150 mg/L) the results revealed that chlorophyll content at p < 0.05 with growth regulator and antioxidant enzymes such as catalase, peroxidase, and malondialdehyde enhanced up to the maximum level at T5 = Hg4ppm + 50 °C (50 °C thermopriming under 4 ppm mercuric chloride stress), suggesting that high temperature initiate the antioxidant system to reduce photosystem damage. However, protein, proline, superoxide dismutase at p < 0.05, and carotenoid, soluble sugar, and ascorbate peroxidase were increased non-significantly (p > 0.05) 50 °C thermopriming under 8 ppm high mercuric chloride stress (T9 = Hg8ppm + 50 °C) representing the tolerance of selected specie by synthesizing osmolytes to resist oxidation mechanism. Furthermore, reduction in % MC (moisture content) is easily improved with foliar application of α-tocopherol and 50 °C thermopriming and 4 ppm heavy metal stress at T6 = Hg4ppm + 50 °C + α-tocopherol (150 mg/L), with a remarkable increase in plant vigor and germination energy. It has resulted that the inhibitory effect of only lower concentration (4 ppm) of heavy metal stress was ameliorated by exogenous application of α-tocopherol and thermopriming technique by synthesizing high levels of proline and antioxidant activities in maintaining seedling growth and development on heavy metal contaminated soil.https://doi.org/10.1186/s12870-024-04767-5Antioxidant enzymesBrassica napus L.ChlorophyllHeavy metalOsmolytes |
| spellingShingle | Fazal Amin Arwa Abdulkreem AL-Huqail Sami Ullah Muhammad Nauman Khan Alevcan Kaplan Baber Ali Majid Iqbal Fahmy Gad Elsaid Sezai Ercisli Tabarak Malik Sami Asir Al-Robai Amany H. A. Abeed Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress BMC Plant Biology Antioxidant enzymes Brassica napus L. Chlorophyll Heavy metal Osmolytes |
| title | Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress |
| title_full | Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress |
| title_fullStr | Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress |
| title_full_unstemmed | Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress |
| title_short | Mitigation effect of alpha-tocopherol and thermo-priming in Brassica napus L. under induced mercuric chloride stress |
| title_sort | mitigation effect of alpha tocopherol and thermo priming in brassica napus l under induced mercuric chloride stress |
| topic | Antioxidant enzymes Brassica napus L. Chlorophyll Heavy metal Osmolytes |
| url | https://doi.org/10.1186/s12870-024-04767-5 |
| work_keys_str_mv | AT fazalamin mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT arwaabdulkreemalhuqail mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT samiullah mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT muhammadnaumankhan mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT alevcankaplan mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT baberali mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT majidiqbal mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT fahmygadelsaid mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT sezaiercisli mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT tabarakmalik mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT samiasiralrobai mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress AT amanyhaabeed mitigationeffectofalphatocopherolandthermopriminginbrassicanapuslunderinducedmercuricchloridestress |