MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma
Abstract Background Increasing numbers of studies have examined the correlation between specific miRNAs and tumours to enable their diagnosis and treatment. However, there are few reports regarding the concrete role and mechanism of miRNA in osteosarcoma. Methods The expression of miR-524 in osteosa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-018-0612-1 |
| _version_ | 1818535399333560320 |
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| author | Ming Zhuang Xubin Qiu Dong Cheng Chenlei Zhu Liang Chen |
| author_facet | Ming Zhuang Xubin Qiu Dong Cheng Chenlei Zhu Liang Chen |
| author_sort | Ming Zhuang |
| collection | DOAJ |
| description | Abstract Background Increasing numbers of studies have examined the correlation between specific miRNAs and tumours to enable their diagnosis and treatment. However, there are few reports regarding the concrete role and mechanism of miRNA in osteosarcoma. Methods The expression of miR-524 in osteosarcoma tissues and cell lines was examined by qRT-PCR. The cell proliferation was examined using CCK-8 in vitro. A series of bioinformatics and molecular biology techniques were adopted to investigate the regulatory relationship between miR-524 and target genes in osteosarcoma. Results The results showed that the miRNA with the most significant differential expression in osteosarcoma was miR-524, which was significantly up-regulated in both osteosarcoma tissues and cell lines. MiR-524 knockdown inhibited proliferation and promoted apoptosis of osteosarcoma cells, while overexpression of miR-524 induced their proliferation. Bioinformatics analysis and luciferase assay confirmed that PTEN was a direct target gene of miR-524 and that miR-524 induced proliferation of osteosarcoma cells through activation of the PI3K/AKT pathway via inhibition of PTEN. Conclusions MiR-524 induces the proliferation of osteosarcoma cells through activation of the PI3K/AKT pathway via inhibition of the target gene PTEN, which provides a theoretical basis for selecting a new therapeutic target for osteosarcoma. |
| first_indexed | 2024-12-11T18:24:21Z |
| format | Article |
| id | doaj.art-7f601789c2d44ec6acecd4b8f274f492 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-11T18:24:21Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-7f601789c2d44ec6acecd4b8f274f4922022-12-22T00:55:08ZengBMCCancer Cell International1475-28672018-08-011811910.1186/s12935-018-0612-1MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcomaMing Zhuang0Xubin Qiu1Dong Cheng2Chenlei Zhu3Liang Chen4Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The Third Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The Third Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The Third Affiliated Hospital of Soochow UniversityDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow UniversityAbstract Background Increasing numbers of studies have examined the correlation between specific miRNAs and tumours to enable their diagnosis and treatment. However, there are few reports regarding the concrete role and mechanism of miRNA in osteosarcoma. Methods The expression of miR-524 in osteosarcoma tissues and cell lines was examined by qRT-PCR. The cell proliferation was examined using CCK-8 in vitro. A series of bioinformatics and molecular biology techniques were adopted to investigate the regulatory relationship between miR-524 and target genes in osteosarcoma. Results The results showed that the miRNA with the most significant differential expression in osteosarcoma was miR-524, which was significantly up-regulated in both osteosarcoma tissues and cell lines. MiR-524 knockdown inhibited proliferation and promoted apoptosis of osteosarcoma cells, while overexpression of miR-524 induced their proliferation. Bioinformatics analysis and luciferase assay confirmed that PTEN was a direct target gene of miR-524 and that miR-524 induced proliferation of osteosarcoma cells through activation of the PI3K/AKT pathway via inhibition of PTEN. Conclusions MiR-524 induces the proliferation of osteosarcoma cells through activation of the PI3K/AKT pathway via inhibition of the target gene PTEN, which provides a theoretical basis for selecting a new therapeutic target for osteosarcoma.http://link.springer.com/article/10.1186/s12935-018-0612-1MicroRNA-524PTENOsteosarcomaPI3K/AKT |
| spellingShingle | Ming Zhuang Xubin Qiu Dong Cheng Chenlei Zhu Liang Chen MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma Cancer Cell International MicroRNA-524 PTEN Osteosarcoma PI3K/AKT |
| title | MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma |
| title_full | MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma |
| title_fullStr | MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma |
| title_full_unstemmed | MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma |
| title_short | MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma |
| title_sort | microrna 524 promotes cell proliferation by down regulating pten expression in osteosarcoma |
| topic | MicroRNA-524 PTEN Osteosarcoma PI3K/AKT |
| url | http://link.springer.com/article/10.1186/s12935-018-0612-1 |
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