Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control
The thermal cycling system is the key component of the real-time fluorescent polymerase chain reaction (PCR) instrument, which determines the nucleic acid detection efficiency and result accuracy. Aiming at the problems that the thermal cycle of the traditional PCR detection system takes a long time...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Shanghai Jiao Tong University
2023-09-01
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| Series: | Shanghai Jiaotong Daxue xuebao |
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| Online Access: | https://xuebao.sjtu.edu.cn/article/2023/1006-2467/1006-2467-57-9-1196.shtml |
| _version_ | 1797672496037625856 |
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| author | CHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming |
| author_facet | CHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming |
| author_sort | CHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming |
| collection | DOAJ |
| description | The thermal cycling system is the key component of the real-time fluorescent polymerase chain reaction (PCR) instrument, which determines the nucleic acid detection efficiency and result accuracy. Aiming at the problems that the thermal cycle of the traditional PCR detection system takes a long time and the temperature control is complicated, a real-time fluorescent PCR thermal cycle system with partition temperature control is designed, including the hardware circuit and mechanical structure of the thermal cycle system. The rapid thermal cycling is achieved by switching the test solution between different constant temperature zones, and the temperature is controlled using an incremental proportional integral derivative (PID) algorithm, with a control precision of ±0.1 ℃. Using Fluent software to establish heat transfer model, the thermal delay phenomenon of the test solution was analyzed to predict the temperature variation pattern of the test solution. A prototype is built for testing and verification, and the heating and cooling rates of the test liquid are 3.8 ℃/s and 4.4 ℃/s. It is verified that the proposed PCR thermal cycling system can effectively improve the detection efficiency. |
| first_indexed | 2024-03-11T21:30:54Z |
| format | Article |
| id | doaj.art-7f60c171f69e4198ae7e07bd539f3345 |
| institution | Directory Open Access Journal |
| issn | 1006-2467 |
| language | zho |
| last_indexed | 2024-03-11T21:30:54Z |
| publishDate | 2023-09-01 |
| publisher | Editorial Office of Journal of Shanghai Jiao Tong University |
| record_format | Article |
| series | Shanghai Jiaotong Daxue xuebao |
| spelling | doaj.art-7f60c171f69e4198ae7e07bd539f33452023-09-27T08:58:38ZzhoEditorial Office of Journal of Shanghai Jiao Tong UniversityShanghai Jiaotong Daxue xuebao1006-24672023-09-015791196120210.16183/j.cnki.jsjtu.2022.361Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature ControlCHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming0School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaThe thermal cycling system is the key component of the real-time fluorescent polymerase chain reaction (PCR) instrument, which determines the nucleic acid detection efficiency and result accuracy. Aiming at the problems that the thermal cycle of the traditional PCR detection system takes a long time and the temperature control is complicated, a real-time fluorescent PCR thermal cycle system with partition temperature control is designed, including the hardware circuit and mechanical structure of the thermal cycle system. The rapid thermal cycling is achieved by switching the test solution between different constant temperature zones, and the temperature is controlled using an incremental proportional integral derivative (PID) algorithm, with a control precision of ±0.1 ℃. Using Fluent software to establish heat transfer model, the thermal delay phenomenon of the test solution was analyzed to predict the temperature variation pattern of the test solution. A prototype is built for testing and verification, and the heating and cooling rates of the test liquid are 3.8 ℃/s and 4.4 ℃/s. It is verified that the proposed PCR thermal cycling system can effectively improve the detection efficiency.https://xuebao.sjtu.edu.cn/article/2023/1006-2467/1006-2467-57-9-1196.shtmlpolymerase chain reaction (pcr) apparatustemperature controlproportional integral derivative (pid) controlfinite element methodheat transfer mechanism |
| spellingShingle | CHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control Shanghai Jiaotong Daxue xuebao polymerase chain reaction (pcr) apparatus temperature control proportional integral derivative (pid) control finite element method heat transfer mechanism |
| title | Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control |
| title_full | Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control |
| title_fullStr | Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control |
| title_full_unstemmed | Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control |
| title_short | Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control |
| title_sort | design of a rapid thermal cycling system for real time fluorescent pcr with zone temperature control |
| topic | polymerase chain reaction (pcr) apparatus temperature control proportional integral derivative (pid) control finite element method heat transfer mechanism |
| url | https://xuebao.sjtu.edu.cn/article/2023/1006-2467/1006-2467-57-9-1196.shtml |
| work_keys_str_mv | AT chenerdonggaozihangwangkundongleihuaming designofarapidthermalcyclingsystemforrealtimefluorescentpcrwithzonetemperaturecontrol |