Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia
Abstract Background The introduction of MALDI-TOF MS in the clinical microbiology laboratory has modified the approaches for the identification of fungi. Thanks to this tool, it is possible to identify cryptic species, which possess critical susceptibility patterns. Clinical strains were identified...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1482-y |
| _version_ | 1818134925305446400 |
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| author | Andrés Ceballos-Garzón Gloria Cortes Florent Morio Edna L. Zamora-Cruz Melva Y. Linares Beatriz E. Ariza Sandra L. Valderrama Javier R. Garzón Carlos A. Alvarez-Moreno Patrice Le Pape Claudia M. Parra-Giraldo |
| author_facet | Andrés Ceballos-Garzón Gloria Cortes Florent Morio Edna L. Zamora-Cruz Melva Y. Linares Beatriz E. Ariza Sandra L. Valderrama Javier R. Garzón Carlos A. Alvarez-Moreno Patrice Le Pape Claudia M. Parra-Giraldo |
| author_sort | Andrés Ceballos-Garzón |
| collection | DOAJ |
| description | Abstract Background The introduction of MALDI-TOF MS in the clinical microbiology laboratory has modified the approaches for the identification of fungi. Thanks to this tool, it is possible to identify cryptic species, which possess critical susceptibility patterns. Clinical strains were identified using the MicroScan and MALDI-TOF MS systems. Discrepant results from both methods were investigated using ITS rDNA barcoding. Finally, these isolates were also tested for in vitro susceptibility. Results The percentage of agreement between both methods to 498 yeast isolates was of 93.6% (32 discrepant isolates). The concordance of ITS sequencing with MALDI-TOF MS was higher (99%) than that of MicroScan (94%). Several of these discordant yeasts displayed high MICs for antifungal agents. Conclusions Our study highlights the need of the MS and molecular approaches such as MALDI-TOF MS and ITS rDNA barcoding for the correct identification of emerging or cryptic yeast species; besides, some of these could be multidrug resistant. This work was the first experience in the implementation of the MALDI-TOF MS technology in Colombia. We found the first uncommon yeasts including Candida auris and we could identify Trichosporon faecalis. Our work highlights a clear necessity of an accurate yeast identification as a much more pertinent technique than the susceptibility profiles, because the most unusual yeasts exhibit resistance profiles to the few available antifungals. |
| first_indexed | 2024-12-11T09:16:22Z |
| format | Article |
| id | doaj.art-7f61e066d5154f52a381ae309b41a254 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T09:16:22Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7f61e066d5154f52a381ae309b41a2542022-12-22T01:13:22ZengBMCBMC Microbiology1471-21802019-05-0119111010.1186/s12866-019-1482-yComparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, ColombiaAndrés Ceballos-Garzón0Gloria Cortes1Florent Morio2Edna L. Zamora-Cruz3Melva Y. Linares4Beatriz E. Ariza5Sandra L. Valderrama6Javier R. Garzón7Carlos A. Alvarez-Moreno8Patrice Le Pape9Claudia M. Parra-Giraldo10Departamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad JaverianaLaboratorio Clínico, Área de Microbiología, Hospital Universitario San IgnacioDepartment of Parasitology and Medical Mycology of the University of Nantes, Nantes Atlantique Universities, Faculty of PharmacyDepartamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad JaverianaDepartamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad JaverianaLaboratorio Clínico, Área de Microbiología, Hospital Universitario San IgnacioUnidad de Infectología, Departamento de Medicina Interna, Facultad de Medicina, Hospital Universitario San IgnacioUnidad de Infectología, Departamento de Medicina Interna, Facultad de Medicina, Hospital Universitario San IgnacioGrupo de Investigación en Enfermedades Infecciosas, Hospital Universitario San IgnacioDepartment of Parasitology and Medical Mycology of the University of Nantes, Nantes Atlantique Universities, Faculty of PharmacyDepartamento de Microbiología, Facultad de Ciencias, Unidad de Proteomica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Pontificia Universidad JaverianaAbstract Background The introduction of MALDI-TOF MS in the clinical microbiology laboratory has modified the approaches for the identification of fungi. Thanks to this tool, it is possible to identify cryptic species, which possess critical susceptibility patterns. Clinical strains were identified using the MicroScan and MALDI-TOF MS systems. Discrepant results from both methods were investigated using ITS rDNA barcoding. Finally, these isolates were also tested for in vitro susceptibility. Results The percentage of agreement between both methods to 498 yeast isolates was of 93.6% (32 discrepant isolates). The concordance of ITS sequencing with MALDI-TOF MS was higher (99%) than that of MicroScan (94%). Several of these discordant yeasts displayed high MICs for antifungal agents. Conclusions Our study highlights the need of the MS and molecular approaches such as MALDI-TOF MS and ITS rDNA barcoding for the correct identification of emerging or cryptic yeast species; besides, some of these could be multidrug resistant. This work was the first experience in the implementation of the MALDI-TOF MS technology in Colombia. We found the first uncommon yeasts including Candida auris and we could identify Trichosporon faecalis. Our work highlights a clear necessity of an accurate yeast identification as a much more pertinent technique than the susceptibility profiles, because the most unusual yeasts exhibit resistance profiles to the few available antifungals.http://link.springer.com/article/10.1186/s12866-019-1482-yComparisonMALDI-TOF MSMicroScanUnusual yeastYeast identification |
| spellingShingle | Andrés Ceballos-Garzón Gloria Cortes Florent Morio Edna L. Zamora-Cruz Melva Y. Linares Beatriz E. Ariza Sandra L. Valderrama Javier R. Garzón Carlos A. Alvarez-Moreno Patrice Le Pape Claudia M. Parra-Giraldo Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia BMC Microbiology Comparison MALDI-TOF MS MicroScan Unusual yeast Yeast identification |
| title | Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia |
| title_full | Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia |
| title_fullStr | Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia |
| title_full_unstemmed | Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia |
| title_short | Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia |
| title_sort | comparison between maldi tof ms and microscan in the identification of emerging and multidrug resistant yeasts in a fourth level hospital in bogota colombia |
| topic | Comparison MALDI-TOF MS MicroScan Unusual yeast Yeast identification |
| url | http://link.springer.com/article/10.1186/s12866-019-1482-y |
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