Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility
In this study, we examined whether smoking and drinking affect sperm quality and the DNA methylation of the repetitive element LINE-1, MEST, P16, H19, and GNAS in sperm. Semen samples were obtained from 143 male residents in a minority-inhabited district of Guizhou province in southwest China. Quant...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.01001/full |
| _version_ | 1828481506915385344 |
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| author | Wenjing Zhang Wenjing Zhang Min Li Feng Sun Xuting Xu Zhaofeng Zhang Junwei Liu Xiaowei Sun Aiping Zhang Yupei Shen Jianhua Xu Maohua Miao Bin Wu Yao Yuan Xianliang Huang Huijuan Shi Jing Du |
| author_facet | Wenjing Zhang Wenjing Zhang Min Li Feng Sun Xuting Xu Zhaofeng Zhang Junwei Liu Xiaowei Sun Aiping Zhang Yupei Shen Jianhua Xu Maohua Miao Bin Wu Yao Yuan Xianliang Huang Huijuan Shi Jing Du |
| author_sort | Wenjing Zhang |
| collection | DOAJ |
| description | In this study, we examined whether smoking and drinking affect sperm quality and the DNA methylation of the repetitive element LINE-1, MEST, P16, H19, and GNAS in sperm. Semen samples were obtained from 143 male residents in a minority-inhabited district of Guizhou province in southwest China. Quantitative DNA methylation analysis of the samples was performed using MassARRAY EpiTYPER assays. Sperm motility was significantly lower in both the nicotine-exposed (P = 0.0064) and the nicotine- and alcohol-exposed (P = 0.0008) groups. Follicle-stimulating hormone (FSH) levels were higher in the nicotine-exposed group (P = 0.0026). The repetitive element LINE-1 was hypermethylated in the three exposed groups, while P16 was hypomethylated in the alcohol and both the alcohol and nicotine exposure groups. Our results also show that alcohol and nicotine exposure altered sperm cell quality, which may be related to the methylation levels of MEST and GNAS. In addition, MEST, GNAS, and the repetitive element LINE1 methylation was significantly associated with the concentration of sperm as well as FSH and luteinizing hormone levels. |
| first_indexed | 2024-12-11T07:58:17Z |
| format | Article |
| id | doaj.art-7f64af7f881c4f1db527ade228492747 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-11T07:58:17Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-7f64af7f881c4f1db527ade2284927472022-12-22T01:15:11ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-10-011010.3389/fgene.2019.01001449417Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of InfertilityWenjing Zhang0Wenjing Zhang1Min Li2Feng Sun3Xuting Xu4Zhaofeng Zhang5Junwei Liu6Xiaowei Sun7Aiping Zhang8Yupei Shen9Jianhua Xu10Maohua Miao11Bin Wu12Yao Yuan13Xianliang Huang14Huijuan Shi15Jing Du16NHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaReproductive Medical Center, Changhai Hospital of Shanghai, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaDepartment of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, ChinaHuzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Zhejiang, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaBio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaShanghai Institute of Planned Parenthood Research Hospital, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaNHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaIn this study, we examined whether smoking and drinking affect sperm quality and the DNA methylation of the repetitive element LINE-1, MEST, P16, H19, and GNAS in sperm. Semen samples were obtained from 143 male residents in a minority-inhabited district of Guizhou province in southwest China. Quantitative DNA methylation analysis of the samples was performed using MassARRAY EpiTYPER assays. Sperm motility was significantly lower in both the nicotine-exposed (P = 0.0064) and the nicotine- and alcohol-exposed (P = 0.0008) groups. Follicle-stimulating hormone (FSH) levels were higher in the nicotine-exposed group (P = 0.0026). The repetitive element LINE-1 was hypermethylated in the three exposed groups, while P16 was hypomethylated in the alcohol and both the alcohol and nicotine exposure groups. Our results also show that alcohol and nicotine exposure altered sperm cell quality, which may be related to the methylation levels of MEST and GNAS. In addition, MEST, GNAS, and the repetitive element LINE1 methylation was significantly associated with the concentration of sperm as well as FSH and luteinizing hormone levels.https://www.frontiersin.org/article/10.3389/fgene.2019.01001/fullDNA methylationnicotine/alcohol exposuremale infertilityimprint genesperm |
| spellingShingle | Wenjing Zhang Wenjing Zhang Min Li Feng Sun Xuting Xu Zhaofeng Zhang Junwei Liu Xiaowei Sun Aiping Zhang Yupei Shen Jianhua Xu Maohua Miao Bin Wu Yao Yuan Xianliang Huang Huijuan Shi Jing Du Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility Frontiers in Genetics DNA methylation nicotine/alcohol exposure male infertility imprint gene sperm |
| title | Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility |
| title_full | Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility |
| title_fullStr | Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility |
| title_full_unstemmed | Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility |
| title_short | Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility |
| title_sort | association of sperm methylation at line 1 four candidate genes and nicotine alcohol exposure with the risk of infertility |
| topic | DNA methylation nicotine/alcohol exposure male infertility imprint gene sperm |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.01001/full |
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