Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates
With the increase of temperature in the winter wheat-growing regions in China, the high-temperature-resistant Blumeria graminis f. sp. tritici (Bgt) isolates developed in the fields. To clarify the key infection stages and the roles of heat shock protein (HSP) genes of high-temperature-resistant Bgt...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1045796/full |
| _version_ | 1798019293224370176 |
|---|---|
| author | Meihui Zhang Meihui Zhang Aolin Wang Cheng Zhang Cheng Zhang Fei Xu Fei Xu Wei Liu Jieru Fan Zhanhong Ma Yilin Zhou |
| author_facet | Meihui Zhang Meihui Zhang Aolin Wang Cheng Zhang Cheng Zhang Fei Xu Fei Xu Wei Liu Jieru Fan Zhanhong Ma Yilin Zhou |
| author_sort | Meihui Zhang |
| collection | DOAJ |
| description | With the increase of temperature in the winter wheat-growing regions in China, the high-temperature-resistant Blumeria graminis f. sp. tritici (Bgt) isolates developed in the fields. To clarify the key infection stages and the roles of heat shock protein (HSP) genes of high-temperature-resistant Bgt isolates defending high temperature, 3 high-temperature-resistant and 3 sensitive Bgt isolates were selected from 55 isolates after determination of temperature sensitivity. And then they were used to investigate the infection stages and the expression levels of HSP genes, including Bgthsp60, Bgthsp70, Bgthsp90, and Bgthsp104, at 18°C and 25°C. The formation frequency of abnormal appressoria and inhibition rate of haustoria formation of high-temperature-resistant isolates at 25°C were lower than those of high-temperature-sensitive isolates, while major axis of microcolonies of high-temperature-resistant isolates was higher than those of high-temperature-sensitive isolates at 25°C. The results indicated that haustoria formation and hyphal expansion were the key infection stages of defense against heat stress in high-temperature-resistant isolates. Further analyses of HSP genes found the expression levels of Bgthsp60 and Bgthsp70c were upregulated at 24 and 72 h post-inoculation in high-temperature-resistant isolates, while no significant difference was observed for Bgthsp90 and Bgthsp104 genes. Taken together, the basis of high-temperature-resistant Bgt isolates is associated with induced expression of Bgthsp60 and Bgthsp70c response to heat stress in haustoria formation and hyphal expansion stages. |
| first_indexed | 2024-04-11T16:38:25Z |
| format | Article |
| id | doaj.art-7f65ae3b07b34a898e770fd312118c52 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T16:38:25Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7f65ae3b07b34a898e770fd312118c522022-12-22T04:13:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10457961045796Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolatesMeihui Zhang0Meihui Zhang1Aolin Wang2Cheng Zhang3Cheng Zhang4Fei Xu5Fei Xu6Wei Liu7Jieru Fan8Zhanhong Ma9Yilin Zhou10State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, College of Agriculture and Foresty Science and Technoloy, Hebei North University, Zhangjiakou, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Southern Part of North China, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Zhengzhou, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaWith the increase of temperature in the winter wheat-growing regions in China, the high-temperature-resistant Blumeria graminis f. sp. tritici (Bgt) isolates developed in the fields. To clarify the key infection stages and the roles of heat shock protein (HSP) genes of high-temperature-resistant Bgt isolates defending high temperature, 3 high-temperature-resistant and 3 sensitive Bgt isolates were selected from 55 isolates after determination of temperature sensitivity. And then they were used to investigate the infection stages and the expression levels of HSP genes, including Bgthsp60, Bgthsp70, Bgthsp90, and Bgthsp104, at 18°C and 25°C. The formation frequency of abnormal appressoria and inhibition rate of haustoria formation of high-temperature-resistant isolates at 25°C were lower than those of high-temperature-sensitive isolates, while major axis of microcolonies of high-temperature-resistant isolates was higher than those of high-temperature-sensitive isolates at 25°C. The results indicated that haustoria formation and hyphal expansion were the key infection stages of defense against heat stress in high-temperature-resistant isolates. Further analyses of HSP genes found the expression levels of Bgthsp60 and Bgthsp70c were upregulated at 24 and 72 h post-inoculation in high-temperature-resistant isolates, while no significant difference was observed for Bgthsp90 and Bgthsp104 genes. Taken together, the basis of high-temperature-resistant Bgt isolates is associated with induced expression of Bgthsp60 and Bgthsp70c response to heat stress in haustoria formation and hyphal expansion stages.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1045796/fullBlumeria graminis f. sp. triticihigh-temperature-resistant isolateinfection stagehistological observationexpression levelheat shock protein |
| spellingShingle | Meihui Zhang Meihui Zhang Aolin Wang Cheng Zhang Cheng Zhang Fei Xu Fei Xu Wei Liu Jieru Fan Zhanhong Ma Yilin Zhou Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates Frontiers in Microbiology Blumeria graminis f. sp. tritici high-temperature-resistant isolate infection stage histological observation expression level heat shock protein |
| title | Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates |
| title_full | Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates |
| title_fullStr | Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates |
| title_full_unstemmed | Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates |
| title_short | Key infection stages defending heat stress in high-temperature-resistant Blumeria graminis f. sp. tritici isolates |
| title_sort | key infection stages defending heat stress in high temperature resistant blumeria graminis f sp tritici isolates |
| topic | Blumeria graminis f. sp. tritici high-temperature-resistant isolate infection stage histological observation expression level heat shock protein |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1045796/full |
| work_keys_str_mv | AT meihuizhang keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT meihuizhang keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT aolinwang keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT chengzhang keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT chengzhang keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT feixu keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT feixu keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT weiliu keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT jierufan keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT zhanhongma keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates AT yilinzhou keyinfectionstagesdefendingheatstressinhightemperatureresistantblumeriagraminisfsptriticiisolates |