Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts
Acacia auriculiformis and A. crassicarpa can be used as non-timber forest products to produce therapeutic agents. To achieve this, it is essential to identify the metabolite profile and biological activity of these raw materials. In this study, a liquid chromatography high resolution mass spectromet...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Saudi Chemical Society |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610323001461 |
| _version_ | 1797378745888145408 |
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| author | Yanico Hadi Prayogo Irmanida Batubara Setyanto Tri Wahyudi Rita Kartika Sari Wasrin Syafii |
| author_facet | Yanico Hadi Prayogo Irmanida Batubara Setyanto Tri Wahyudi Rita Kartika Sari Wasrin Syafii |
| author_sort | Yanico Hadi Prayogo |
| collection | DOAJ |
| description | Acacia auriculiformis and A. crassicarpa can be used as non-timber forest products to produce therapeutic agents. To achieve this, it is essential to identify the metabolite profile and biological activity of these raw materials. In this study, a liquid chromatography high resolution mass spectrometry platform was used to identify metabolites in the leaf, bark, and heartwood extracts that were positively related to antioxidant and antiproliferative activity using in vitro and in silico studies. A. crassicarpa heartwood extract showed significantly higher antioxidant activity compared to the other extracts. Moreover, the leaf and heartwood extracts from A. auriculiformis, as well as the heartwood extracts from A. crassicarpa, showed significantly high antiproliferative activity. Multivariate analysis using an orthogonal partial least squares model identified several metabolites as significant marker and differentiator compounds between the two Acacia species, with a positive correlation to biological activity. The molecular docking technique predicted various positions of hydroxyl substitution on the flavonoids from A. crassicarpa and A. auriculiformis that alter their binding energy to target receptors, which are related to oxidative stress and cancer treatment targets. Moreover, the methylated flavonoid molecules provide less binding energy. These flavonoids and their derivatives were predominantly found in the heartwood extracts, which demonstrates their potential for further exploration using this part of A. crassicarpa and A. auriculiformis. |
| first_indexed | 2024-03-08T20:12:09Z |
| format | Article |
| id | doaj.art-7f65d693dd0b411bab72fb0836b8b48d |
| institution | Directory Open Access Journal |
| issn | 1319-6103 |
| language | English |
| last_indexed | 2024-03-08T20:12:09Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj.art-7f65d693dd0b411bab72fb0836b8b48d2023-12-23T05:20:07ZengElsevierJournal of Saudi Chemical Society1319-61032023-11-01276101742Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extractsYanico Hadi Prayogo0Irmanida Batubara1Setyanto Tri Wahyudi2Rita Kartika Sari3Wasrin Syafii4Department of Forest Products, Faculty of Forestry and Environment, IPB University, Bogor 16680, IndonesiaDepartment of Chemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor 16680, Indonesia; Tropical Biopharmaca Research Center, IPB University, Bogor 16128, IndonesiaTropical Biopharmaca Research Center, IPB University, Bogor 16128, Indonesia; Department of Physics, Faculty of Mathematic and Natural Science, IPB University, Bogor 16680, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Bogor 16680, Indonesia; Tropical Biopharmaca Research Center, IPB University, Bogor 16128, Indonesia; Advanced Research Laboratory, IPB University, Bogor 16680, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Bogor 16680, Indonesia; Corresponding author.Acacia auriculiformis and A. crassicarpa can be used as non-timber forest products to produce therapeutic agents. To achieve this, it is essential to identify the metabolite profile and biological activity of these raw materials. In this study, a liquid chromatography high resolution mass spectrometry platform was used to identify metabolites in the leaf, bark, and heartwood extracts that were positively related to antioxidant and antiproliferative activity using in vitro and in silico studies. A. crassicarpa heartwood extract showed significantly higher antioxidant activity compared to the other extracts. Moreover, the leaf and heartwood extracts from A. auriculiformis, as well as the heartwood extracts from A. crassicarpa, showed significantly high antiproliferative activity. Multivariate analysis using an orthogonal partial least squares model identified several metabolites as significant marker and differentiator compounds between the two Acacia species, with a positive correlation to biological activity. The molecular docking technique predicted various positions of hydroxyl substitution on the flavonoids from A. crassicarpa and A. auriculiformis that alter their binding energy to target receptors, which are related to oxidative stress and cancer treatment targets. Moreover, the methylated flavonoid molecules provide less binding energy. These flavonoids and their derivatives were predominantly found in the heartwood extracts, which demonstrates their potential for further exploration using this part of A. crassicarpa and A. auriculiformis.http://www.sciencedirect.com/science/article/pii/S1319610323001461AcaciaMetabolomicsProliferation inhibitionAntioxidantMolecular docking |
| spellingShingle | Yanico Hadi Prayogo Irmanida Batubara Setyanto Tri Wahyudi Rita Kartika Sari Wasrin Syafii Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts Journal of Saudi Chemical Society Acacia Metabolomics Proliferation inhibition Antioxidant Molecular docking |
| title | Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts |
| title_full | Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts |
| title_fullStr | Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts |
| title_full_unstemmed | Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts |
| title_short | Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts |
| title_sort | metabolite profile antiproliferative and antioxidant activity of acacia auriculiformis and acacia crassicarpa extracts |
| topic | Acacia Metabolomics Proliferation inhibition Antioxidant Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S1319610323001461 |
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