A high-performance polymer composite column for coronavirus nucleic acid purification
Abstract Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including SARS-CoV-2, in 82 clinical samples. The r...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51671-x |
| _version_ | 1827382205908779008 |
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| author | Akli Zarouri Aaron M. T. Barnes Hamada Aboubakr Vinni Thekkudan Novi Qiuchen Dong Andrew Nelson Sagar Goyal Abdennour Abbas |
| author_facet | Akli Zarouri Aaron M. T. Barnes Hamada Aboubakr Vinni Thekkudan Novi Qiuchen Dong Andrew Nelson Sagar Goyal Abdennour Abbas |
| author_sort | Akli Zarouri |
| collection | DOAJ |
| description | Abstract Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including SARS-CoV-2, in 82 clinical samples. The results shows that PC-based purification outperforms silica gel (SG)-based purification by enabling a higher sensitivity (94%), accuracy (97%), and by eliminating false positives (100% specificity). The high specificity is critical for efficient patient triage and resource management during pandemics. Furthermore, PC-based purification exhibits three times higher analytical precision than a commonly used SG-based nucleic acid purification thereby enabling a more accurate quantification of viral loads and higher reproducibility. |
| first_indexed | 2024-03-08T14:16:12Z |
| format | Article |
| id | doaj.art-7f66179be1f045d88ebcbabbe39d50d3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:16:12Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7f66179be1f045d88ebcbabbe39d50d32024-01-14T12:23:06ZengNature PortfolioScientific Reports2045-23222024-01-011411710.1038/s41598-024-51671-xA high-performance polymer composite column for coronavirus nucleic acid purificationAkli Zarouri0Aaron M. T. Barnes1Hamada Aboubakr2Vinni Thekkudan Novi3Qiuchen Dong4Andrew Nelson5Sagar Goyal6Abdennour Abbas7Department of Bioproducts and Biosystems Engineering, University of Minnesota Twin CitiesDivision of Molecular Pathology and Genomics, Department of Laboratory Medicine and Pathology, University of Minnesota Twin CitiesDepartment of Bioproducts and Biosystems Engineering, University of Minnesota Twin CitiesDepartment of Bioproducts and Biosystems Engineering, University of Minnesota Twin CitiesDepartment of Bioproducts and Biosystems Engineering, University of Minnesota Twin CitiesDivision of Molecular Pathology and Genomics, Department of Laboratory Medicine and Pathology, University of Minnesota Twin CitiesDepartment of Veterinary Population Medicine, University of Minnesota Twin CitiesDepartment of Bioproducts and Biosystems Engineering, University of Minnesota Twin CitiesAbstract Here, we report the development of a novel polymer composite (PC) purification column and kit. The performance of the PC columns was compared to conventional silica gel (SG) columns for the purification of nucleic acids from coronaviruses, including SARS-CoV-2, in 82 clinical samples. The results shows that PC-based purification outperforms silica gel (SG)-based purification by enabling a higher sensitivity (94%), accuracy (97%), and by eliminating false positives (100% specificity). The high specificity is critical for efficient patient triage and resource management during pandemics. Furthermore, PC-based purification exhibits three times higher analytical precision than a commonly used SG-based nucleic acid purification thereby enabling a more accurate quantification of viral loads and higher reproducibility.https://doi.org/10.1038/s41598-024-51671-x |
| spellingShingle | Akli Zarouri Aaron M. T. Barnes Hamada Aboubakr Vinni Thekkudan Novi Qiuchen Dong Andrew Nelson Sagar Goyal Abdennour Abbas A high-performance polymer composite column for coronavirus nucleic acid purification Scientific Reports |
| title | A high-performance polymer composite column for coronavirus nucleic acid purification |
| title_full | A high-performance polymer composite column for coronavirus nucleic acid purification |
| title_fullStr | A high-performance polymer composite column for coronavirus nucleic acid purification |
| title_full_unstemmed | A high-performance polymer composite column for coronavirus nucleic acid purification |
| title_short | A high-performance polymer composite column for coronavirus nucleic acid purification |
| title_sort | high performance polymer composite column for coronavirus nucleic acid purification |
| url | https://doi.org/10.1038/s41598-024-51671-x |
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