Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia
Abstract The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolate...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-11-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01134-z |
| _version_ | 1818341669109497856 |
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| author | Maroua Oueslati Magdalena Mulet Mohamed Zouaoui Charlotte Chandeysson Jorge Lalucat Mohamed Rabeh Hajlaoui Odile Berge Elena García-Valdés Najla Sadfi-Zouaoui |
| author_facet | Maroua Oueslati Magdalena Mulet Mohamed Zouaoui Charlotte Chandeysson Jorge Lalucat Mohamed Rabeh Hajlaoui Odile Berge Elena García-Valdés Najla Sadfi-Zouaoui |
| author_sort | Maroua Oueslati |
| collection | DOAJ |
| description | Abstract The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means. |
| first_indexed | 2024-12-13T16:02:28Z |
| format | Article |
| id | doaj.art-7f6800a377e247739334039a154a2031 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T16:02:28Z |
| publishDate | 2020-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-7f6800a377e247739334039a154a20312022-12-21T23:39:08ZengSpringerOpenAMB Express2191-08552020-11-0110112010.1186/s13568-020-01134-zDiversity of pathogenic Pseudomonas isolated from citrus in TunisiaMaroua Oueslati0Magdalena Mulet1Mohamed Zouaoui2Charlotte Chandeysson3Jorge Lalucat4Mohamed Rabeh Hajlaoui5Odile Berge6Elena García-Valdés7Najla Sadfi-Zouaoui8Laboratoire de Mycologie, Pathologies et Biomarqueurs (LR16ES05), Département de Biologie, Université de Tunis-El ManarMicrobiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de Les Illes BalearsLaboratoire de Mycologie, Pathologies et Biomarqueurs (LR16ES05), Département de Biologie, Université de Tunis-El ManarINRAE, Pathologie VégétaleMicrobiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de Les Illes BalearsLaboratoire de Biotechnologie Appliquée à l’Agriculture, INRA Tunisia, Université de CarthageINRAE, Pathologie VégétaleMicrobiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de Les Illes BalearsLaboratoire de Mycologie, Pathologies et Biomarqueurs (LR16ES05), Département de Biologie, Université de Tunis-El ManarAbstract The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.http://link.springer.com/article/10.1186/s13568-020-01134-zCitrusCultivarsPseudomonas syringaerpoDPathogenicity |
| spellingShingle | Maroua Oueslati Magdalena Mulet Mohamed Zouaoui Charlotte Chandeysson Jorge Lalucat Mohamed Rabeh Hajlaoui Odile Berge Elena García-Valdés Najla Sadfi-Zouaoui Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia AMB Express Citrus Cultivars Pseudomonas syringae rpoD Pathogenicity |
| title | Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia |
| title_full | Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia |
| title_fullStr | Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia |
| title_full_unstemmed | Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia |
| title_short | Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia |
| title_sort | diversity of pathogenic pseudomonas isolated from citrus in tunisia |
| topic | Citrus Cultivars Pseudomonas syringae rpoD Pathogenicity |
| url | http://link.springer.com/article/10.1186/s13568-020-01134-z |
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