The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion
Background: Transplantation of bone marrow mesenchymal stem cells (BMSCs) has a promising therapeutic efficiency for varieties of disorders caused by ischemia or reperfusion impairment. It has been shown that BMSCs can mitigate intestinal ischemia/reperfusion (I/R) injuries, but the underlying mecha...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023027925 |
| _version_ | 1797815639269703680 |
|---|---|
| author | He Wang Kun Wang Bo Liu Xiaoqian Bian Xiaojie Tan Haitao Jiang |
| author_facet | He Wang Kun Wang Bo Liu Xiaoqian Bian Xiaojie Tan Haitao Jiang |
| author_sort | He Wang |
| collection | DOAJ |
| description | Background: Transplantation of bone marrow mesenchymal stem cells (BMSCs) has a promising therapeutic efficiency for varieties of disorders caused by ischemia or reperfusion impairment. It has been shown that BMSCs can mitigate intestinal ischemia/reperfusion (I/R) injuries, but the underlying mechanism is still unclear. This study aimed at investigating the efficacy of BMSCs on the immune function of intestinal mucosal microenvironment after I/R injuries. Methods: Twenty adult Sprague-Dawley rats were randomly assigned to a treatment or a control group. All the rats underwent superior mesenteric artery clamping and unclamping. In the treatment group, BMSCs were implanted into the intestine of ten rats by direct submucosal injection whereas the other ten rats in the control group were injected with the same volume of saline. On the fourth and seventh day after BMSCs transplantation, intestinal samples were examined for the CD4 (CD4-positive T-lymphocytes)/CD8 (CD8-positive T-lymphocytes) ratio of the bowel mucosa via flow cytometry, and for the level of Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Interleukin-6 (IL-6) via ELISA. Paneth cell counts and Secretory Immunoglobulin A (SIgA) level were examined via immunohistochemical (IHC) analysis. Real time PCR (RT-PCR) was used to detect the expression levels of tumor necrosis factor-α (TNF-α) and trypsinogen (Serine 2) (PRSS2) genes. White blood cell (WBC) count was measured by manual counting under the microscope. Results: The CD4/CD8 ratio in the treatment group was significantly lower compared with that in the control group. The concentration of IL-2 and IL-6 was lower in the treatment group compared with the control group, while the level of IL-4 is the reverse between the two groups. The number of Paneth cells in intestinal mucosa increased significantly, while the level of SIgA in intestinal mucosa decreased significantly, after BMSCs transplantation. The gene expression levels of TNF-α and PRSS2 in intestinal mucosa of treatment group were significantly lower than those of control group. The WBC count in the treatment group was significantly lower than that in the control group. Conclusion: We identified immune-relevant molecular changes that may explain the mechanism of BMSCs transplantation efficacy in alleviating rat intestinal immune-barrier after I/R. |
| first_indexed | 2024-03-13T08:26:52Z |
| format | Article |
| id | doaj.art-7f69950ebe8c415e9b6f46257dd0c3db |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:26:52Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7f69950ebe8c415e9b6f46257dd0c3db2023-05-31T04:44:50ZengElsevierHeliyon2405-84402023-05-0195e15585The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusionHe Wang0Kun Wang1Bo Liu2Xiaoqian Bian3Xiaojie Tan4Haitao Jiang5Department of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, ChinaThe Second Clinical Medical College, Lanzhou University, Lanzhou, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Qingdao University, China; Corresponding author. No. 16 Jiangsu Road, Qingdao, Shandong Province, 266003, China.Background: Transplantation of bone marrow mesenchymal stem cells (BMSCs) has a promising therapeutic efficiency for varieties of disorders caused by ischemia or reperfusion impairment. It has been shown that BMSCs can mitigate intestinal ischemia/reperfusion (I/R) injuries, but the underlying mechanism is still unclear. This study aimed at investigating the efficacy of BMSCs on the immune function of intestinal mucosal microenvironment after I/R injuries. Methods: Twenty adult Sprague-Dawley rats were randomly assigned to a treatment or a control group. All the rats underwent superior mesenteric artery clamping and unclamping. In the treatment group, BMSCs were implanted into the intestine of ten rats by direct submucosal injection whereas the other ten rats in the control group were injected with the same volume of saline. On the fourth and seventh day after BMSCs transplantation, intestinal samples were examined for the CD4 (CD4-positive T-lymphocytes)/CD8 (CD8-positive T-lymphocytes) ratio of the bowel mucosa via flow cytometry, and for the level of Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Interleukin-6 (IL-6) via ELISA. Paneth cell counts and Secretory Immunoglobulin A (SIgA) level were examined via immunohistochemical (IHC) analysis. Real time PCR (RT-PCR) was used to detect the expression levels of tumor necrosis factor-α (TNF-α) and trypsinogen (Serine 2) (PRSS2) genes. White blood cell (WBC) count was measured by manual counting under the microscope. Results: The CD4/CD8 ratio in the treatment group was significantly lower compared with that in the control group. The concentration of IL-2 and IL-6 was lower in the treatment group compared with the control group, while the level of IL-4 is the reverse between the two groups. The number of Paneth cells in intestinal mucosa increased significantly, while the level of SIgA in intestinal mucosa decreased significantly, after BMSCs transplantation. The gene expression levels of TNF-α and PRSS2 in intestinal mucosa of treatment group were significantly lower than those of control group. The WBC count in the treatment group was significantly lower than that in the control group. Conclusion: We identified immune-relevant molecular changes that may explain the mechanism of BMSCs transplantation efficacy in alleviating rat intestinal immune-barrier after I/R.http://www.sciencedirect.com/science/article/pii/S2405844023027925Mesenchymal stem cellsIntestinal mucosal immune barrierIschemia reperfusion injuryRepair mechanism |
| spellingShingle | He Wang Kun Wang Bo Liu Xiaoqian Bian Xiaojie Tan Haitao Jiang The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion Heliyon Mesenchymal stem cells Intestinal mucosal immune barrier Ischemia reperfusion injury Repair mechanism |
| title | The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion |
| title_full | The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion |
| title_fullStr | The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion |
| title_full_unstemmed | The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion |
| title_short | The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion |
| title_sort | efficacy of bone marrow mesenchymal stem cells on rat intestinal immune function injured by ischemia reperfusion |
| topic | Mesenchymal stem cells Intestinal mucosal immune barrier Ischemia reperfusion injury Repair mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023027925 |
| work_keys_str_mv | AT hewang theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT kunwang theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT boliu theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT xiaoqianbian theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT xiaojietan theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT haitaojiang theefficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT hewang efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT kunwang efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT boliu efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT xiaoqianbian efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT xiaojietan efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion AT haitaojiang efficacyofbonemarrowmesenchymalstemcellsonratintestinalimmunefunctioninjuredbyischemiareperfusion |