Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway
Accumulating pieces of evidence showed that α-enolase (ENO1) is a multifunctional protein that plays a crucial role in a variety of pathophysiological processes. In our previous study, differential expression of ENO1 was observed in different heat-tolerance duck breeds. Here, we examined in vitro ex...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.693780/full |
| _version_ | 1819152472897224704 |
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| author | Tao Zeng Tao Zeng Yongqing Cao Tiantian Gu Tiantian Gu Li Chen Yong Tian Yong Tian Guoqin Li Guoqin Li Junda Shen Zhenrong Tao Lizhi Lu Lizhi Lu |
| author_facet | Tao Zeng Tao Zeng Yongqing Cao Tiantian Gu Tiantian Gu Li Chen Yong Tian Yong Tian Guoqin Li Guoqin Li Junda Shen Zhenrong Tao Lizhi Lu Lizhi Lu |
| author_sort | Tao Zeng |
| collection | DOAJ |
| description | Accumulating pieces of evidence showed that α-enolase (ENO1) is a multifunctional protein that plays a crucial role in a variety of pathophysiological processes. In our previous study, differential expression of ENO1 was observed in different heat-tolerance duck breeds. Here, we examined in vitro expression level of ENO1 in hepatocytes against heat stress. The mechanisms of ENO1 on cell glycolysis, growth, and its potential regulatory pathways were also analyzed. The results showed that ENO1 expression in messenger RNA and protein levels were both greatly increased in heat-treated cells compared with non-treated cells. ENO1-overexpressed cells significantly elevated cell viability and glycolysis levels. It was further shown that stably upregulated ENO1 activated focal adhesion kinase-phosphatidylinositol 3-kinase/Akt and its downstream signals. In addition, the interaction between ENO1 and 70-kDa heat shock protein was detected using co-immunoprecipitation. Our research suggests that ENO1 may interact with 70-kDa heat shock protein to protect hepatocyte against heat stress through focal adhesion kinase-mediated phosphatidylinositol 3-kinase/Akt pathway. |
| first_indexed | 2024-12-22T14:49:51Z |
| format | Article |
| id | doaj.art-7f6a1676c9ce4789a172c733a2ebc426 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-22T14:49:51Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-7f6a1676c9ce4789a172c733a2ebc4262022-12-21T18:22:21ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-07-011210.3389/fgene.2021.693780693780Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt PathwayTao Zeng0Tao Zeng1Yongqing Cao2Tiantian Gu3Tiantian Gu4Li Chen5Yong Tian6Yong Tian7Guoqin Li8Guoqin Li9Junda Shen10Zhenrong Tao11Lizhi Lu12Lizhi Lu13Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaJiangsu Key Laboratory for Animal Genetics, Breeding and Molecular Design, Yangzhou University, Yangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaKey Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou, ChinaAccumulating pieces of evidence showed that α-enolase (ENO1) is a multifunctional protein that plays a crucial role in a variety of pathophysiological processes. In our previous study, differential expression of ENO1 was observed in different heat-tolerance duck breeds. Here, we examined in vitro expression level of ENO1 in hepatocytes against heat stress. The mechanisms of ENO1 on cell glycolysis, growth, and its potential regulatory pathways were also analyzed. The results showed that ENO1 expression in messenger RNA and protein levels were both greatly increased in heat-treated cells compared with non-treated cells. ENO1-overexpressed cells significantly elevated cell viability and glycolysis levels. It was further shown that stably upregulated ENO1 activated focal adhesion kinase-phosphatidylinositol 3-kinase/Akt and its downstream signals. In addition, the interaction between ENO1 and 70-kDa heat shock protein was detected using co-immunoprecipitation. Our research suggests that ENO1 may interact with 70-kDa heat shock protein to protect hepatocyte against heat stress through focal adhesion kinase-mediated phosphatidylinositol 3-kinase/Akt pathway.https://www.frontiersin.org/articles/10.3389/fgene.2021.693780/fullENO1Hsp70glycolysiscell viabilityFAK-PI3K/AKT |
| spellingShingle | Tao Zeng Tao Zeng Yongqing Cao Tiantian Gu Tiantian Gu Li Chen Yong Tian Yong Tian Guoqin Li Guoqin Li Junda Shen Zhenrong Tao Lizhi Lu Lizhi Lu Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway Frontiers in Genetics ENO1 Hsp70 glycolysis cell viability FAK-PI3K/AKT |
| title | Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway |
| title_full | Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway |
| title_fullStr | Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway |
| title_full_unstemmed | Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway |
| title_short | Alpha-Enolase Protects Hepatocyte Against Heat Stress Through Focal Adhesion Kinase-Mediated Phosphatidylinositol 3-Kinase/Akt Pathway |
| title_sort | alpha enolase protects hepatocyte against heat stress through focal adhesion kinase mediated phosphatidylinositol 3 kinase akt pathway |
| topic | ENO1 Hsp70 glycolysis cell viability FAK-PI3K/AKT |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.693780/full |
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