Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats
Objective(s): Uterine ischemia is a common problem with ongoing controversy about its pathogenesis and prevention. The present study aimed to investigate the protective role of sitagliptin against uterine ischemia-reperfusion injury (IRI). Materials and Methods: Rats were allocated into 4 groups: co...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-11-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_21108_e57ff6f9d2fb1481522198206c9f666e.pdf |
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| author | Remon Rofaeil Sabreen Ahmed Haitham Bahaa Ahmad Mahran Nermeen Welson Walaa Abdelzaher |
| author_facet | Remon Rofaeil Sabreen Ahmed Haitham Bahaa Ahmad Mahran Nermeen Welson Walaa Abdelzaher |
| author_sort | Remon Rofaeil |
| collection | DOAJ |
| description | Objective(s): Uterine ischemia is a common problem with ongoing controversy about its pathogenesis and prevention. The present study aimed to investigate the protective role of sitagliptin against uterine ischemia-reperfusion injury (IRI). Materials and Methods: Rats were allocated into 4 groups: control, sitagliptin (SIT) (5 mg/kg), IR; ischemia was induced followed by reperfusion, and IR+SIT; SIT was administered 1 hr before IRI. Uteri were removed for histopathological and biochemical observations. Malondialdehyde (MDA), total nitrites (NOx), reduced glutathione (GSH), superoxide dismutase (SOD) activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and toll-like receptor 4 (TLR4) were all measured. Hematoxylin and eosin (H&E) stain, Periodic acid-Schiff stain (PAS), and caspase-3 immunostaining were applied. Results: In the IR+SIT group; NOx, GSH, and SOD activities increased significantly. Meanwhile, the levels of MDA, TNF-α, IL-6, TLR4, and caspase-3 immunoexpression showed a significant reduction, as compared with the IR group. In the IR+SIT group, an improvement in the histopathological picture was noticed.Conclusion: The results showed that sitagliptin confers protection against uterine IRI through anti-oxidant, anti-inflammatory, and anti-apoptotic effects with a possible role for TLR4. |
| first_indexed | 2024-04-11T23:54:12Z |
| format | Article |
| id | doaj.art-7f6a40b0c4f44dafad9b785859bdaf2b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T23:54:12Z |
| publishDate | 2022-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-7f6a40b0c4f44dafad9b785859bdaf2b2022-12-22T03:56:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-11-0125111396140110.22038/ijbms.2022.64552.1420221108Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in ratsRemon Rofaeil0Sabreen Ahmed1Haitham Bahaa2Ahmad Mahran3Nermeen Welson4Walaa Abdelzaher5Department of Pharmacology, Faculty of Medicine, Minia University, Minia, EgyptDepatment of Human Anatomy and Embryology, Faculty of Medicine, Minia University, delegated to Deraya University-New Minia City, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, Minia University. Minia, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, Minia University. Minia, EgyptDepartment of Forensic medicine and clinical toxicology, Faculty of Medicine, Beni-Suef University, Beni-Suef, EgyptDepartment of Pharmacology, Faculty of Medicine, Minia University, Minia, EgyptObjective(s): Uterine ischemia is a common problem with ongoing controversy about its pathogenesis and prevention. The present study aimed to investigate the protective role of sitagliptin against uterine ischemia-reperfusion injury (IRI). Materials and Methods: Rats were allocated into 4 groups: control, sitagliptin (SIT) (5 mg/kg), IR; ischemia was induced followed by reperfusion, and IR+SIT; SIT was administered 1 hr before IRI. Uteri were removed for histopathological and biochemical observations. Malondialdehyde (MDA), total nitrites (NOx), reduced glutathione (GSH), superoxide dismutase (SOD) activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and toll-like receptor 4 (TLR4) were all measured. Hematoxylin and eosin (H&E) stain, Periodic acid-Schiff stain (PAS), and caspase-3 immunostaining were applied. Results: In the IR+SIT group; NOx, GSH, and SOD activities increased significantly. Meanwhile, the levels of MDA, TNF-α, IL-6, TLR4, and caspase-3 immunoexpression showed a significant reduction, as compared with the IR group. In the IR+SIT group, an improvement in the histopathological picture was noticed.Conclusion: The results showed that sitagliptin confers protection against uterine IRI through anti-oxidant, anti-inflammatory, and anti-apoptotic effects with a possible role for TLR4.https://ijbms.mums.ac.ir/article_21108_e57ff6f9d2fb1481522198206c9f666e.pdfanti-apoptoticanti-inflammatorysitagliptintoll-like receptor 4uterine ischemia |
| spellingShingle | Remon Rofaeil Sabreen Ahmed Haitham Bahaa Ahmad Mahran Nermeen Welson Walaa Abdelzaher Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats Iranian Journal of Basic Medical Sciences anti-apoptotic anti-inflammatory sitagliptin toll-like receptor 4 uterine ischemia |
| title | Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats |
| title_full | Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats |
| title_fullStr | Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats |
| title_full_unstemmed | Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats |
| title_short | Dipeptidyl peptidase-4 inhibitor: Sitagliptin down-regulated toll-like receptor 4 signaling pathway to reduce uterine injury in rats |
| title_sort | dipeptidyl peptidase 4 inhibitor sitagliptin down regulated toll like receptor 4 signaling pathway to reduce uterine injury in rats |
| topic | anti-apoptotic anti-inflammatory sitagliptin toll-like receptor 4 uterine ischemia |
| url | https://ijbms.mums.ac.ir/article_21108_e57ff6f9d2fb1481522198206c9f666e.pdf |
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