Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient
Background and Objective: Present study aimed to investigate response of metallothionein (MT) in oyster Crassostrea sp. to the experimental concentrations of Hg and Cd in order to assess the possibility of MT usage as a biomarker of Hg and Cd contamination in this mollusk. Materials and Methods: Oys...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2014-05-01
|
| Series: | سلامت و محیط |
| Subjects: | |
| Online Access: | http://ijhe.tums.ac.ir/browse.php?a_code=A-10-77-3&slc_lang=en&sid=1 |
| _version_ | 1818690078456676352 |
|---|---|
| author | A. Azimi A. Safahieh A. Dadollahi Sohrab H. Zolgharnein A. Savari B. Saffar |
| author_facet | A. Azimi A. Safahieh A. Dadollahi Sohrab H. Zolgharnein A. Savari B. Saffar |
| author_sort | A. Azimi |
| collection | DOAJ |
| description | Background and Objective: Present study aimed to investigate response of metallothionein (MT) in oyster Crassostrea sp. to the experimental concentrations of Hg and Cd in order to assess the possibility of MT usage as a biomarker of Hg and Cd contamination in this mollusk.
Materials and Methods: Oysters were collected from docks of Imam Khomeini Port. After seven days acclimation period in laboratory, they were exposed with Hg at concentrations of 15 and 75 μg.L-1 and Cd at concentrations of 15 and 150 μg.L-1 for 14 days. MT levels were measured through spectrophotometric method after extraction and precipitation. After preparation and acid digestion, the concentration of heavy metals was quantified by atomic absorption.
Results: The amounts of MT in oysters exposed with Hg and Cd were significantly increased compared with control samples (P<0.05). The highest value of MT biosynthesis in oysters exposed with concentrations of Hg and Cd were 137.2±7.6 and 312.4±17.9 μg.g-1 w.w respectively. Exposing the oysters with Cd induced biosynthesis of MT more than three times compared with control samples. Among Hg and Cd, there was only significant correlation between biosynthesis of MT and Cd bioaccumulation in oysters (P<0.01).
Conclusion: MT protein in oyster Crassostrea sp. can be considered as a suitable biomarker of Cd contamination in body and environment. Hence, it could be used for assessing and monitoring ecosystems. |
| first_indexed | 2024-12-17T12:20:17Z |
| format | Article |
| id | doaj.art-7f6a5cefa34b4633a91e0b64b7ac5752 |
| institution | Directory Open Access Journal |
| issn | 2008-2029 2008-3718 |
| language | fas |
| last_indexed | 2024-12-17T12:20:17Z |
| publishDate | 2014-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | سلامت و محیط |
| spelling | doaj.art-7f6a5cefa34b4633a91e0b64b7ac57522022-12-21T21:48:59ZfasTehran University of Medical Sciencesسلامت و محیط2008-20292008-37182014-05-0173413426Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradientA. Azimi0A. Safahieh1A. Dadollahi Sohrab2H. Zolgharnein3A. Savari4B. Saffar5 Iranian National Institute for Oceanography and Atmospheric Sciences (INIOAS), Tehran Iran, Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Department of Environment, Faculty of Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Department of Marine Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Department of Genetic, Faculty of Basic Sciences, Shahrekord University. Shahrekord. Iran Background and Objective: Present study aimed to investigate response of metallothionein (MT) in oyster Crassostrea sp. to the experimental concentrations of Hg and Cd in order to assess the possibility of MT usage as a biomarker of Hg and Cd contamination in this mollusk. Materials and Methods: Oysters were collected from docks of Imam Khomeini Port. After seven days acclimation period in laboratory, they were exposed with Hg at concentrations of 15 and 75 μg.L-1 and Cd at concentrations of 15 and 150 μg.L-1 for 14 days. MT levels were measured through spectrophotometric method after extraction and precipitation. After preparation and acid digestion, the concentration of heavy metals was quantified by atomic absorption. Results: The amounts of MT in oysters exposed with Hg and Cd were significantly increased compared with control samples (P<0.05). The highest value of MT biosynthesis in oysters exposed with concentrations of Hg and Cd were 137.2±7.6 and 312.4±17.9 μg.g-1 w.w respectively. Exposing the oysters with Cd induced biosynthesis of MT more than three times compared with control samples. Among Hg and Cd, there was only significant correlation between biosynthesis of MT and Cd bioaccumulation in oysters (P<0.01). Conclusion: MT protein in oyster Crassostrea sp. can be considered as a suitable biomarker of Cd contamination in body and environment. Hence, it could be used for assessing and monitoring ecosystems.http://ijhe.tums.ac.ir/browse.php?a_code=A-10-77-3&slc_lang=en&sid=1Biomarker Metallothionein mercury cadmium exposure Oyster Crassostrea sp |
| spellingShingle | A. Azimi A. Safahieh A. Dadollahi Sohrab H. Zolgharnein A. Savari B. Saffar Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient سلامت و محیط Biomarker Metallothionein mercury cadmium exposure Oyster Crassostrea sp |
| title | Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient |
| title_full | Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient |
| title_fullStr | Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient |
| title_full_unstemmed | Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient |
| title_short | Response of Metallothionein biomarker in oyster Crassostrea sp. to the mercury and cadmium gradient |
| title_sort | response of metallothionein biomarker in oyster crassostrea sp to the mercury and cadmium gradient |
| topic | Biomarker Metallothionein mercury cadmium exposure Oyster Crassostrea sp |
| url | http://ijhe.tums.ac.ir/browse.php?a_code=A-10-77-3&slc_lang=en&sid=1 |
| work_keys_str_mv | AT aazimi responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient AT asafahieh responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient AT adadollahisohrab responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient AT hzolgharnein responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient AT asavari responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient AT bsaffar responseofmetallothioneinbiomarkerinoystercrassostreasptothemercuryandcadmiumgradient |