In vitro regeneration of Didymopanax morototoni
The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant m...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842006000300011&lng=en&tlng=en |
| _version_ | 1819102909606920192 |
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| author | E. T. H. Franco L. B. Gavioli A. G. Ferreira |
| author_facet | E. T. H. Franco L. B. Gavioli A. G. Ferreira |
| author_sort | E. T. H. Franco |
| collection | DOAJ |
| description | The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1). Twenty days after inoculation, the material was evaluated. Embryogenic calli were split, transferred to expression medium with several combinations of NAA and KIN, and moved to fresh medium after 60 days. Light did not interfere in embryo expression. Somatic embryos were formed either from individual cells or cell clusters. Plantlets were obtained in WPM medium and 10 gL-1 of sucrose with no plant regulator, or using 0.1 mgL-1 BAP and 0.5 mgL-1GA. Plantlets from somatic embryos of D. morototoni developed in 33% of the cases. |
| first_indexed | 2024-12-22T01:42:03Z |
| format | Article |
| id | doaj.art-7f70158b28d7434eba07cf8f413d6282 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-22T01:42:03Z |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-7f70158b28d7434eba07cf8f413d62822022-12-21T18:43:11ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-4375662a45546210.1590/S1519-69842006000300011S1519-69842006000300011In vitro regeneration of Didymopanax morototoniE. T. H. Franco0L. B. Gavioli1A. G. Ferreira2Universidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal do Rio Grande do SulThe present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1). Twenty days after inoculation, the material was evaluated. Embryogenic calli were split, transferred to expression medium with several combinations of NAA and KIN, and moved to fresh medium after 60 days. Light did not interfere in embryo expression. Somatic embryos were formed either from individual cells or cell clusters. Plantlets were obtained in WPM medium and 10 gL-1 of sucrose with no plant regulator, or using 0.1 mgL-1 BAP and 0.5 mgL-1GA. Plantlets from somatic embryos of D. morototoni developed in 33% of the cases.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842006000300011&lng=en&tlng=ensomatic embryowoody plantsembryogenesisplantletsSchefflera (Didymopanax)Araliaceae |
| spellingShingle | E. T. H. Franco L. B. Gavioli A. G. Ferreira In vitro regeneration of Didymopanax morototoni Brazilian Journal of Biology somatic embryo woody plants embryogenesis plantlets Schefflera (Didymopanax) Araliaceae |
| title | In vitro regeneration of Didymopanax morototoni |
| title_full | In vitro regeneration of Didymopanax morototoni |
| title_fullStr | In vitro regeneration of Didymopanax morototoni |
| title_full_unstemmed | In vitro regeneration of Didymopanax morototoni |
| title_short | In vitro regeneration of Didymopanax morototoni |
| title_sort | in vitro regeneration of didymopanax morototoni |
| topic | somatic embryo woody plants embryogenesis plantlets Schefflera (Didymopanax) Araliaceae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842006000300011&lng=en&tlng=en |
| work_keys_str_mv | AT ethfranco invitroregenerationofdidymopanaxmorototoni AT lbgavioli invitroregenerationofdidymopanaxmorototoni AT agferreira invitroregenerationofdidymopanaxmorototoni |