Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation

Two different process configurations, simultaneous saccharification and fermentation (SSF), and separate hydrolysis and fermentation (SHF), were compared for ethanol production from Shorgum bicolor grain. Optimization modeling for glucoamylase and Zymomonas mobilis concentration in both of SSF and S...

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Main Authors: RUTH CHRISNASARI, DAMIATI HARTINI SUSETYO, ADRIAN PRATAMA SUGIANTO, TJANDRA PANTJAJANI
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2013-05-01
Series:Microbiology Indonesia
Subjects:
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/205
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author RUTH CHRISNASARI
DAMIATI HARTINI SUSETYO
ADRIAN PRATAMA SUGIANTO
TJANDRA PANTJAJANI
author_facet RUTH CHRISNASARI
DAMIATI HARTINI SUSETYO
ADRIAN PRATAMA SUGIANTO
TJANDRA PANTJAJANI
author_sort RUTH CHRISNASARI
collection DOAJ
description Two different process configurations, simultaneous saccharification and fermentation (SSF), and separate hydrolysis and fermentation (SHF), were compared for ethanol production from Shorgum bicolor grain. Optimization modeling for glucoamylase and Zymomonas mobilis concentration in both of SSF and SHF were carried out to obtain optimal concentration of ethanol production. The optimum condition was achieved using 0.021 % (v/v) of glucoamylase, and 30.19% (v/v) of Z. mobilis for SHF. In contrast, the optimum condition for SSF was 0.021 % (v/v) of glucoamylase and 17.51% (v/v) of Z. mobilis. The model predicted SHF processing to be superior. The superiority of SHF over SSF was confirmed experimentally, the result showed ethanol yield of SHF was 134.80 g L-1 and ethanol yield of SSF was 115.66 g L-1 after 72 h incubation time. A high similarity was observed between the predicted and experimental results, demonstrating the accuracy of the model.
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spelling doaj.art-7f74106d241e44b3bb2d9616082562fc2022-12-21T20:06:26ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752013-05-017110.5454/mi.7.1.2Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification FermentationRUTH CHRISNASARI0DAMIATI HARTINI SUSETYO1ADRIAN PRATAMA SUGIANTO2TJANDRA PANTJAJANI3Department of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, IndonesiaDepartment of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, IndonesiaDepartment of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, IndonesiaDepartment of Biology, Faculty of Biotechnology, Universitas Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, IndonesiaTwo different process configurations, simultaneous saccharification and fermentation (SSF), and separate hydrolysis and fermentation (SHF), were compared for ethanol production from Shorgum bicolor grain. Optimization modeling for glucoamylase and Zymomonas mobilis concentration in both of SSF and SHF were carried out to obtain optimal concentration of ethanol production. The optimum condition was achieved using 0.021 % (v/v) of glucoamylase, and 30.19% (v/v) of Z. mobilis for SHF. In contrast, the optimum condition for SSF was 0.021 % (v/v) of glucoamylase and 17.51% (v/v) of Z. mobilis. The model predicted SHF processing to be superior. The superiority of SHF over SSF was confirmed experimentally, the result showed ethanol yield of SHF was 134.80 g L-1 and ethanol yield of SSF was 115.66 g L-1 after 72 h incubation time. A high similarity was observed between the predicted and experimental results, demonstrating the accuracy of the model.https://jurnal.permi.or.id/index.php/mionline/article/view/205ethanolShorgum bicolorseparate hydrolysis and fermentationsimultaneous saccharification and fermentation
spellingShingle RUTH CHRISNASARI
DAMIATI HARTINI SUSETYO
ADRIAN PRATAMA SUGIANTO
TJANDRA PANTJAJANI
Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
Microbiology Indonesia
ethanol
Shorgum bicolor
separate hydrolysis and fermentation
simultaneous saccharification and fermentation
title Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
title_full Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
title_fullStr Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
title_full_unstemmed Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
title_short Optimization Modeling of Ethanol Production from Shorgum bicolor grain: Comparison between Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation
title_sort optimization modeling of ethanol production from shorgum bicolor grain comparison between separate hydrolysis fermentation and simultaneous saccharification fermentation
topic ethanol
Shorgum bicolor
separate hydrolysis and fermentation
simultaneous saccharification and fermentation
url https://jurnal.permi.or.id/index.php/mionline/article/view/205
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