Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency
During translation, intracellular mRNA folds co-transcriptionally and must refold following the passage of ribosome. The mRNAs can be entrapped in metastable structures during these folding events. In the present study, we evaluated the conformational dynamics of the kinetically favored, metastable,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1613 |
| _version_ | 1818982180131438592 |
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| author | Tamaki Endoh Naoki Sugimoto |
| author_facet | Tamaki Endoh Naoki Sugimoto |
| author_sort | Tamaki Endoh |
| collection | DOAJ |
| description | During translation, intracellular mRNA folds co-transcriptionally and must refold following the passage of ribosome. The mRNAs can be entrapped in metastable structures during these folding events. In the present study, we evaluated the conformational dynamics of the kinetically favored, metastable, and hairpin-like structure, which disturbs the thermodynamically favored G-quadruplex structure, and its effect on co-transcriptional translation in prokaryotic cells. We found that nascent mRNA forms a metastable hairpin-like structure during co-transcriptional folding instead of the G-quadruplex structure. When the translation progressed co-transcriptionally before the metastable hairpin-like structure transition to the G-quadruplex, function of the G-quadruplex as a roadblock of the ribosome was sequestered. This suggested that kinetically formed RNA structures had a dominant effect on gene expression in prokaryotes. The results of this study indicate that it is critical to consider the conformational dynamics of RNA-folding to understand the contributions of the mRNA structures in controlling gene expression. |
| first_indexed | 2024-12-20T17:43:07Z |
| format | Article |
| id | doaj.art-7f79fd2e472c4f298f3066f059ace750 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T17:43:07Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7f79fd2e472c4f298f3066f059ace7502022-12-21T19:31:02ZengMDPI AGMolecules1420-30492019-04-01248161310.3390/molecules24081613molecules24081613Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation EfficiencyTamaki Endoh0Naoki Sugimoto1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanFrontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDuring translation, intracellular mRNA folds co-transcriptionally and must refold following the passage of ribosome. The mRNAs can be entrapped in metastable structures during these folding events. In the present study, we evaluated the conformational dynamics of the kinetically favored, metastable, and hairpin-like structure, which disturbs the thermodynamically favored G-quadruplex structure, and its effect on co-transcriptional translation in prokaryotic cells. We found that nascent mRNA forms a metastable hairpin-like structure during co-transcriptional folding instead of the G-quadruplex structure. When the translation progressed co-transcriptionally before the metastable hairpin-like structure transition to the G-quadruplex, function of the G-quadruplex as a roadblock of the ribosome was sequestered. This suggested that kinetically formed RNA structures had a dominant effect on gene expression in prokaryotes. The results of this study indicate that it is critical to consider the conformational dynamics of RNA-folding to understand the contributions of the mRNA structures in controlling gene expression.https://www.mdpi.com/1420-3049/24/8/1613conformational dynamicsco-transcriptional foldingco-translational refoldingmetastable structureG-quadruplextranslation suppression |
| spellingShingle | Tamaki Endoh Naoki Sugimoto Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency Molecules conformational dynamics co-transcriptional folding co-translational refolding metastable structure G-quadruplex translation suppression |
| title | Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency |
| title_full | Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency |
| title_fullStr | Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency |
| title_full_unstemmed | Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency |
| title_short | Conformational Dynamics of the RNA G-Quadruplex and its Effect on Translation Efficiency |
| title_sort | conformational dynamics of the rna g quadruplex and its effect on translation efficiency |
| topic | conformational dynamics co-transcriptional folding co-translational refolding metastable structure G-quadruplex translation suppression |
| url | https://www.mdpi.com/1420-3049/24/8/1613 |
| work_keys_str_mv | AT tamakiendoh conformationaldynamicsofthernagquadruplexanditseffectontranslationefficiency AT naokisugimoto conformationaldynamicsofthernagquadruplexanditseffectontranslationefficiency |