Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing
Hu sheep, a locally bred species in China known for its high productivity, is currently suffering from pneumonia. Here, we combine high-throughput 16SrRNA gene sequencing and bacterial culturing to examine the bacterial community in pneumonic Hu Sheep lungs (<i>p</i> < 0.05). The resu...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/17/2763 |
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| author | Yongqiang Miao Xueliang Zhao Jianlin Lei Jingru Ding Hang Feng Ke Wu Jiaohu Liu Chunyang Wang Dongyang Ye Xinglong Wang Juan Wang Zengqi Yang |
| author_facet | Yongqiang Miao Xueliang Zhao Jianlin Lei Jingru Ding Hang Feng Ke Wu Jiaohu Liu Chunyang Wang Dongyang Ye Xinglong Wang Juan Wang Zengqi Yang |
| author_sort | Yongqiang Miao |
| collection | DOAJ |
| description | Hu sheep, a locally bred species in China known for its high productivity, is currently suffering from pneumonia. Here, we combine high-throughput 16SrRNA gene sequencing and bacterial culturing to examine the bacterial community in pneumonic Hu Sheep lungs (<i>p</i> < 0.05). The results showed that the abundance and diversity of lung bacteria in healthy sheep were significantly higher than those in pneumonia sheep (<i>p</i> = 0.139), while there was no significant difference between moderate and severe pneumonia. Furthermore, the composition of the lung microbiota community underwent significant alterations between different levels of pneumonia severity. The application of LEfSe analysis revealed a notable enrichment of <i>Mannheimiae</i> within the lungs of sheep afflicted with moderate pneumonia (<i>p</i> < 0.01), surpassing the levels observed in their healthy counterparts. Additionally, <i>Fusobacterium</i> emerged as the prevailing bacterial group within the lungs of sheep suffering from severe pneumonia. Integrating the results of bacterial isolation and identification, we conclusively determined that <i>Mannheimia haemolytica</i> was the primary pathogenic bacterium within the lungs of sheep afflicted with moderate pneumonia. Furthermore, the exacerbation of pneumonia may be attributed to the synergistic interplay between <i>Fusobacterium</i> spp. and other bacterial species. Our results provide new insights for guiding preventive and therapeutic measures for pneumonia of different severities in sheep. |
| first_indexed | 2024-03-10T23:28:44Z |
| format | Article |
| id | doaj.art-7f7a2c42a8de42ebb6ed6ba69340207c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:28:44Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7f7a2c42a8de42ebb6ed6ba69340207c2023-11-19T07:47:03ZengMDPI AGAnimals2076-26152023-08-011317276310.3390/ani13172763Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene SequencingYongqiang Miao0Xueliang Zhao1Jianlin Lei2Jingru Ding3Hang Feng4Ke Wu5Jiaohu Liu6Chunyang Wang7Dongyang Ye8Xinglong Wang9Juan Wang10Zengqi Yang11College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaHu sheep, a locally bred species in China known for its high productivity, is currently suffering from pneumonia. Here, we combine high-throughput 16SrRNA gene sequencing and bacterial culturing to examine the bacterial community in pneumonic Hu Sheep lungs (<i>p</i> < 0.05). The results showed that the abundance and diversity of lung bacteria in healthy sheep were significantly higher than those in pneumonia sheep (<i>p</i> = 0.139), while there was no significant difference between moderate and severe pneumonia. Furthermore, the composition of the lung microbiota community underwent significant alterations between different levels of pneumonia severity. The application of LEfSe analysis revealed a notable enrichment of <i>Mannheimiae</i> within the lungs of sheep afflicted with moderate pneumonia (<i>p</i> < 0.01), surpassing the levels observed in their healthy counterparts. Additionally, <i>Fusobacterium</i> emerged as the prevailing bacterial group within the lungs of sheep suffering from severe pneumonia. Integrating the results of bacterial isolation and identification, we conclusively determined that <i>Mannheimia haemolytica</i> was the primary pathogenic bacterium within the lungs of sheep afflicted with moderate pneumonia. Furthermore, the exacerbation of pneumonia may be attributed to the synergistic interplay between <i>Fusobacterium</i> spp. and other bacterial species. Our results provide new insights for guiding preventive and therapeutic measures for pneumonia of different severities in sheep.https://www.mdpi.com/2076-2615/13/17/2763Hu sheeppneumonia<i>Mannheimia</i>microbiome16SrRNA sequencing |
| spellingShingle | Yongqiang Miao Xueliang Zhao Jianlin Lei Jingru Ding Hang Feng Ke Wu Jiaohu Liu Chunyang Wang Dongyang Ye Xinglong Wang Juan Wang Zengqi Yang Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing Animals Hu sheep pneumonia <i>Mannheimia</i> microbiome 16SrRNA sequencing |
| title | Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing |
| title_full | Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing |
| title_fullStr | Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing |
| title_full_unstemmed | Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing |
| title_short | Characterization of Lung Microbiomes in Pneumonic Hu Sheep Using Culture Technique and 16S rRNA Gene Sequencing |
| title_sort | characterization of lung microbiomes in pneumonic hu sheep using culture technique and 16s rrna gene sequencing |
| topic | Hu sheep pneumonia <i>Mannheimia</i> microbiome 16SrRNA sequencing |
| url | https://www.mdpi.com/2076-2615/13/17/2763 |
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