Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia
Bacterial microbiota in stool may vary over a wide range, depending on age, nutrition, etc. The purpose of our work was to discriminate phyla and genera of intestinal bacteria and their biodiversity within a healthy population (North-Western Russia) compared to the patients with type 1 diabetes mell...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/7/1813 |
| _version_ | 1797588098669871104 |
|---|---|
| author | Alexei B. Chukhlovin Vasilisa V. Dudurich Aleksey V. Kusakin Dmitry E. Polev Ekaterina D. Ermachenko Mikhail V. Aseev Yuri A. Zakharov Yuri A. Eismont Lavrentii G. Danilov Oleg S. Glotov |
| author_facet | Alexei B. Chukhlovin Vasilisa V. Dudurich Aleksey V. Kusakin Dmitry E. Polev Ekaterina D. Ermachenko Mikhail V. Aseev Yuri A. Zakharov Yuri A. Eismont Lavrentii G. Danilov Oleg S. Glotov |
| author_sort | Alexei B. Chukhlovin |
| collection | DOAJ |
| description | Bacterial microbiota in stool may vary over a wide range, depending on age, nutrition, etc. The purpose of our work was to discriminate phyla and genera of intestinal bacteria and their biodiversity within a healthy population (North-Western Russia) compared to the patients with type 1 diabetes mellitus (T1DM). The study group included 183 healthy persons 2 to 53 years old (a mean of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>26.5</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.0</mn></mrow></semantics></math></inline-formula> years old), and 41 T1DM patients (mean age <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>18.2</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.8</mn></mrow></semantics></math></inline-formula> years old). The disease onset was at <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>11</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.5</mn></mrow></semantics></math></inline-formula> years, with a T1DM experience of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>7</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.5</mn></mrow></semantics></math></inline-formula> years. Total DNA was isolated from the stool samples, and sequencing libraries were prepared by amplifying the V3–V4 region of the 16S rRNA gene sequenced by Illumina MiSeq. Bioinformatic processing of NGS databases was adapted for microbiota evalutaion. Despite the broad scatter, the biological diversity for bacterial microbiota expressed as the Shannon index was significantly increased from younger to older ages in the comparison group, higher in adult healthy persons, with a trend for decrease in the <i>Actinomycetota</i> phylum which includes <i>Bifidobacterium longum</i> species. Similar but non-significant age trends were noted in the T1DM group. Concordant with the <i>Bacillota</i> prevalence in stool samples of diabetic patients, some anaerobic bacteria (<i>Faecalibacteria</i>, <i>Lachnospira</i> and <i>Ruminococcae</i>, <i>Roseburia</i>) were enriched in the T1DM microbiome against controls. Hence, correction of microbiota for <i>Ruminococcus</i> and <i>Lachnospiraceae</i> requires future search for new probiotics. Lower abundance of <i>Actinomycetota</i> and <i>Bifidobacter</i> in T1DM suggests potential usage of Bifidobacter-based probiotics in this cohort. |
| first_indexed | 2024-03-11T00:48:00Z |
| format | Article |
| id | doaj.art-7f7d2738ac2440ef9dbc2f21f406225c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:48:00Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7f7d2738ac2440ef9dbc2f21f406225c2023-11-18T20:36:48ZengMDPI AGMicroorganisms2076-26072023-07-01117181310.3390/microorganisms11071813Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western RussiaAlexei B. Chukhlovin0Vasilisa V. Dudurich1Aleksey V. Kusakin2Dmitry E. Polev3Ekaterina D. Ermachenko4Mikhail V. Aseev5Yuri A. Zakharov6Yuri A. Eismont7Lavrentii G. Danilov8Oleg S. Glotov9R.M.Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov First Saint Petersburg State Medical University, 197022 St. Petersburg, RussiaSerbalab Laboratory, 199106 St. Petersburg, RussiaR.M.Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov First Saint Petersburg State Medical University, 197022 St. Petersburg, RussiaSerbalab Laboratory, 199106 St. Petersburg, RussiaSerbalab Laboratory, 199106 St. Petersburg, RussiaSerbalab Laboratory, 199106 St. Petersburg, RussiaCanadian Institute for Regenerative Medicine, Toronto, ON M5G OA3, CanadaR.M.Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov First Saint Petersburg State Medical University, 197022 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint-Petersburg State University, Universitetskaya Nab. 7/9, 199034 St. Petersburg, RussiaPediatric Research and Clinical Center for Infectious Diseases, 197022 St. Petersburg, RussiaBacterial microbiota in stool may vary over a wide range, depending on age, nutrition, etc. The purpose of our work was to discriminate phyla and genera of intestinal bacteria and their biodiversity within a healthy population (North-Western Russia) compared to the patients with type 1 diabetes mellitus (T1DM). The study group included 183 healthy persons 2 to 53 years old (a mean of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>26.5</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.0</mn></mrow></semantics></math></inline-formula> years old), and 41 T1DM patients (mean age <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>18.2</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.8</mn></mrow></semantics></math></inline-formula> years old). The disease onset was at <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>11</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.5</mn></mrow></semantics></math></inline-formula> years, with a T1DM experience of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>7</mn><mspace width="3.33333pt"></mspace><mo>±</mo><mspace width="3.33333pt"></mspace><mn>1.5</mn></mrow></semantics></math></inline-formula> years. Total DNA was isolated from the stool samples, and sequencing libraries were prepared by amplifying the V3–V4 region of the 16S rRNA gene sequenced by Illumina MiSeq. Bioinformatic processing of NGS databases was adapted for microbiota evalutaion. Despite the broad scatter, the biological diversity for bacterial microbiota expressed as the Shannon index was significantly increased from younger to older ages in the comparison group, higher in adult healthy persons, with a trend for decrease in the <i>Actinomycetota</i> phylum which includes <i>Bifidobacterium longum</i> species. Similar but non-significant age trends were noted in the T1DM group. Concordant with the <i>Bacillota</i> prevalence in stool samples of diabetic patients, some anaerobic bacteria (<i>Faecalibacteria</i>, <i>Lachnospira</i> and <i>Ruminococcae</i>, <i>Roseburia</i>) were enriched in the T1DM microbiome against controls. Hence, correction of microbiota for <i>Ruminococcus</i> and <i>Lachnospiraceae</i> requires future search for new probiotics. Lower abundance of <i>Actinomycetota</i> and <i>Bifidobacter</i> in T1DM suggests potential usage of Bifidobacter-based probiotics in this cohort.https://www.mdpi.com/2076-2607/11/7/1813microbiotabacterialbiodiversitystool16S rRNA sequencingdiabetes mellitus |
| spellingShingle | Alexei B. Chukhlovin Vasilisa V. Dudurich Aleksey V. Kusakin Dmitry E. Polev Ekaterina D. Ermachenko Mikhail V. Aseev Yuri A. Zakharov Yuri A. Eismont Lavrentii G. Danilov Oleg S. Glotov Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia Microorganisms microbiota bacterial biodiversity stool 16S rRNA sequencing diabetes mellitus |
| title | Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia |
| title_full | Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia |
| title_fullStr | Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia |
| title_full_unstemmed | Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia |
| title_short | Evaluation of Gut Microbiota in Healthy Persons and Type 1 Diabetes Mellitus Patients in North-Western Russia |
| title_sort | evaluation of gut microbiota in healthy persons and type 1 diabetes mellitus patients in north western russia |
| topic | microbiota bacterial biodiversity stool 16S rRNA sequencing diabetes mellitus |
| url | https://www.mdpi.com/2076-2607/11/7/1813 |
| work_keys_str_mv | AT alexeibchukhlovin evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT vasilisavdudurich evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT alekseyvkusakin evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT dmitryepolev evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT ekaterinadermachenko evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT mikhailvaseev evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT yuriazakharov evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT yuriaeismont evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT lavrentiigdanilov evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia AT olegsglotov evaluationofgutmicrobiotainhealthypersonsandtype1diabetesmellituspatientsinnorthwesternrussia |