Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways
<strong><em>Objective(s):</em></strong> Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon can...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_15520_46c1eecdd2eb476f7b2ac707a5de09e2.pdf |
| _version_ | 1811266411538939904 |
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| author | Siamak Alizadeh Abolghasem Esmaeili Yadollah Omidi |
| author_facet | Siamak Alizadeh Abolghasem Esmaeili Yadollah Omidi |
| author_sort | Siamak Alizadeh |
| collection | DOAJ |
| description | <strong><em>Objective(s):</em></strong> Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon cancer are yet to be fully understood. The present study assessed the anti-cancer effects of EcN treatments in human colorectal cancer, HT-29 cell line, with the analysis of related mechanisms. <br /><em><strong>Materials and Methods:</strong> </em>The co-culture conditioned-media (CM) of EcN with adenocarcinoma HT-29 cells and heat-inactivated bacteria (HIB) were applied for the treatment of the HT-29 cells. To study the inhibition potential of CM and HIB on cancer cells, various cellular/molecular analyses were implemented, including DAPI-staining and DNA ladder assays, flow cytometry and Real-time quantitative PCR (qPCR), as well as Western blotting analyses.<br /><em><strong>Results:</strong> </em>Our results indicated that EcN could elicit apoptotic impacts on the colon cancer HT-29 cells by up-regulating PTEN and Bax and down-regulating AKT1 and Bcl-xL genes. <br /><em><strong>Conclusion:</strong></em> Based on our findings, EcN is proposed as a useful supplemental probiotic treatment against colon cancer. |
| first_indexed | 2024-04-12T20:41:41Z |
| format | Article |
| id | doaj.art-7f8186929c9748668f16c4dad47ba55d |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T20:41:41Z |
| publishDate | 2020-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-7f8186929c9748668f16c4dad47ba55d2022-12-22T03:17:23ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-07-0123788689310.22038/ijbms.2020.43016.1011515520Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathwaysSiamak Alizadeh0Abolghasem Esmaeili1Yadollah Omidi2Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran|Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, IranResearch Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran|Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran<strong><em>Objective(s):</em></strong> Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon cancer are yet to be fully understood. The present study assessed the anti-cancer effects of EcN treatments in human colorectal cancer, HT-29 cell line, with the analysis of related mechanisms. <br /><em><strong>Materials and Methods:</strong> </em>The co-culture conditioned-media (CM) of EcN with adenocarcinoma HT-29 cells and heat-inactivated bacteria (HIB) were applied for the treatment of the HT-29 cells. To study the inhibition potential of CM and HIB on cancer cells, various cellular/molecular analyses were implemented, including DAPI-staining and DNA ladder assays, flow cytometry and Real-time quantitative PCR (qPCR), as well as Western blotting analyses.<br /><em><strong>Results:</strong> </em>Our results indicated that EcN could elicit apoptotic impacts on the colon cancer HT-29 cells by up-regulating PTEN and Bax and down-regulating AKT1 and Bcl-xL genes. <br /><em><strong>Conclusion:</strong></em> Based on our findings, EcN is proposed as a useful supplemental probiotic treatment against colon cancer.http://ijbms.mums.ac.ir/article_15520_46c1eecdd2eb476f7b2ac707a5de09e2.pdfaktapoptosiscell signalingcolon cancerescherichia coli nissle1917 |
| spellingShingle | Siamak Alizadeh Abolghasem Esmaeili Yadollah Omidi Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways Iranian Journal of Basic Medical Sciences akt apoptosis cell signaling colon cancer escherichia coli nissle1917 |
| title | Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways |
| title_full | Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways |
| title_fullStr | Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways |
| title_full_unstemmed | Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways |
| title_short | Anti-cancer properties of Escherichia coli Nissle 1917 against HT-29 colon cancer cells through regulation of Bax/Bcl-xL and AKT/PTEN signaling pathways |
| title_sort | anti cancer properties of escherichia coli nissle 1917 against ht 29 colon cancer cells through regulation of bax bcl xl and akt pten signaling pathways |
| topic | akt apoptosis cell signaling colon cancer escherichia coli nissle1917 |
| url | http://ijbms.mums.ac.ir/article_15520_46c1eecdd2eb476f7b2ac707a5de09e2.pdf |
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