Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts
The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/11/2117 |
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| author | Juan Shong Khaw Christopher R. Bowen Sarah H. Cartmell |
| author_facet | Juan Shong Khaw Christopher R. Bowen Sarah H. Cartmell |
| author_sort | Juan Shong Khaw |
| collection | DOAJ |
| description | The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO<sub>2</sub> nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO<sub>2</sub> nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation. |
| first_indexed | 2024-03-10T15:21:47Z |
| format | Article |
| id | doaj.art-7f8419b9379b461a85bd8806955e5551 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T15:21:47Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7f8419b9379b461a85bd8806955e55512023-11-20T18:26:53ZengMDPI AGNanomaterials2079-49912020-10-011011211710.3390/nano10112117Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human OsteoblastsJuan Shong Khaw0Christopher R. Bowen1Sarah H. Cartmell2Department of Materials, The University of Manchester, Manchester M13 9PL, UKDepartment of Mechanical Engineering, University of Bath, Bath BA2 7AY, UKDepartment of Materials, The University of Manchester, Manchester M13 9PL, UKThe pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO<sub>2</sub> nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO<sub>2</sub> nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation.https://www.mdpi.com/2079-4991/10/11/2117nanotubepore diametersurface nanotopographystem cell osteogenesisosteogenic differentiationosteoblastic maturation |
| spellingShingle | Juan Shong Khaw Christopher R. Bowen Sarah H. Cartmell Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts Nanomaterials nanotube pore diameter surface nanotopography stem cell osteogenesis osteogenic differentiation osteoblastic maturation |
| title | Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts |
| title_full | Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts |
| title_fullStr | Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts |
| title_full_unstemmed | Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts |
| title_short | Effect of TiO<sub>2</sub> Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts |
| title_sort | effect of tio sub 2 sub nanotube pore diameter on human mesenchymal stem cells and human osteoblasts |
| topic | nanotube pore diameter surface nanotopography stem cell osteogenesis osteogenic differentiation osteoblastic maturation |
| url | https://www.mdpi.com/2079-4991/10/11/2117 |
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