Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice
Objective To investigate the effects of methionine restriction (MR) on macrophages in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to explore the underlying mechanism. Methods According to the random number table method, 36 male C57BL/6J mice (6~8 weeks old, 23±2 g) were divided int...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Army Medical University
2024-04-01
|
| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/html/202308112.htm |
| _version_ | 1797205644114132992 |
|---|---|
| author | LIAO Xianjian WEN Jing DUAN Jiaxiang |
| author_facet | LIAO Xianjian WEN Jing DUAN Jiaxiang |
| author_sort | LIAO Xianjian |
| collection | DOAJ |
| description | Objective To investigate the effects of methionine restriction (MR) on macrophages in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to explore the underlying mechanism. Methods According to the random number table method, 36 male C57BL/6J mice (6~8 weeks old, 23±2 g) were divided into 3 groups with 12 mice in each group: the sham group, the LPS group and the LPS+MR group. HE staining and pathological scoring of lung injury were performed in lung tissues. The expression of LPS-binding protein (LBP) and Toll-like receptor-4 (TLR4) was detected by RT-qPCR and Western blotting. Macrophage-colony stimulating factor (M-CSF), granulocyte-macrophage-colony stimulating factor (GM-CSF) and chemokine C-C motif ligand 3(CCL3) which are all macrophage-associated chemokines were analyzed by immunohistochemistry. Results Compared with the sham group, the pathological score of lung injury in the LPS group was significantly increased (P < 0.01);The mRNA and protein expression levels of LBP and TLR4 were significantly increased; The number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were significantly increased (P < 0.01). MR significantly improved LPS-induced ALI, and decreased the pathological score of lung injury (P < 0.01); The mRNA and protein expression levels of LBP and TLR4 were decreased; Compared with the LPS group, the number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were reduced in the LPS+MR group (P < 0.01). Conclusion MR could attenuate LPS-induced ALI by inhibiting the expression of macrophage chemokines and preventing infiltration and activation of macrophage to lungs.
|
| first_indexed | 2024-04-24T08:54:24Z |
| format | Article |
| id | doaj.art-7f86a0d909664140a2ba34ec44bef9cf |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-24T08:54:24Z |
| publishDate | 2024-04-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-7f86a0d909664140a2ba34ec44bef9cf2024-04-16T08:43:57ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272024-04-0146768869410.16016/j.2097-0927.202308112Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in miceLIAO Xianjian0 WEN Jing1DUAN Jiaxiang2Department of Anaesthesiology, First Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Anaesthesiology, First Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Anaesthesiology, First Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400038, China Objective To investigate the effects of methionine restriction (MR) on macrophages in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to explore the underlying mechanism. Methods According to the random number table method, 36 male C57BL/6J mice (6~8 weeks old, 23±2 g) were divided into 3 groups with 12 mice in each group: the sham group, the LPS group and the LPS+MR group. HE staining and pathological scoring of lung injury were performed in lung tissues. The expression of LPS-binding protein (LBP) and Toll-like receptor-4 (TLR4) was detected by RT-qPCR and Western blotting. Macrophage-colony stimulating factor (M-CSF), granulocyte-macrophage-colony stimulating factor (GM-CSF) and chemokine C-C motif ligand 3(CCL3) which are all macrophage-associated chemokines were analyzed by immunohistochemistry. Results Compared with the sham group, the pathological score of lung injury in the LPS group was significantly increased (P < 0.01);The mRNA and protein expression levels of LBP and TLR4 were significantly increased; The number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were significantly increased (P < 0.01). MR significantly improved LPS-induced ALI, and decreased the pathological score of lung injury (P < 0.01); The mRNA and protein expression levels of LBP and TLR4 were decreased; Compared with the LPS group, the number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were reduced in the LPS+MR group (P < 0.01). Conclusion MR could attenuate LPS-induced ALI by inhibiting the expression of macrophage chemokines and preventing infiltration and activation of macrophage to lungs. http://aammt.tmmu.edu.cn/html/202308112.htmacute lung injurymacrophagemethionine restrictionchemokineslipopolysaccharide |
| spellingShingle | LIAO Xianjian WEN Jing DUAN Jiaxiang Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice 陆军军医大学学报 acute lung injury macrophage methionine restriction chemokines lipopolysaccharide |
| title | Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice |
| title_full | Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice |
| title_fullStr | Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice |
| title_full_unstemmed | Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice |
| title_short | Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice |
| title_sort | effects and mechanism of methionine restriction on macrophage for lipopolysaccharide induced acute lung injury in mice |
| topic | acute lung injury macrophage methionine restriction chemokines lipopolysaccharide |
| url | http://aammt.tmmu.edu.cn/html/202308112.htm |
| work_keys_str_mv | AT liaoxianjian effectsandmechanismofmethioninerestrictiononmacrophageforlipopolysaccharideinducedacutelunginjuryinmice AT wenjing effectsandmechanismofmethioninerestrictiononmacrophageforlipopolysaccharideinducedacutelunginjuryinmice AT duanjiaxiang effectsandmechanismofmethioninerestrictiononmacrophageforlipopolysaccharideinducedacutelunginjuryinmice |