Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium
ABSTRACTCardiomyocytes derived from human pluripotent stem cells (hPSCs) can be used in various applications including disease modeling, drug safety screening, and novel cell-based cardiac therapies. Here, we report an optimized selection and maturation method to induce maturation of cardiomyocytes...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2023.2189932 |
| _version_ | 1797361643382898688 |
|---|---|
| author | Ji-eun Kim Eun-Mi Kim Hyang-Ae Lee Ki-Suk Kim |
| author_facet | Ji-eun Kim Eun-Mi Kim Hyang-Ae Lee Ki-Suk Kim |
| author_sort | Ji-eun Kim |
| collection | DOAJ |
| description | ABSTRACTCardiomyocytes derived from human pluripotent stem cells (hPSCs) can be used in various applications including disease modeling, drug safety screening, and novel cell-based cardiac therapies. Here, we report an optimized selection and maturation method to induce maturation of cardiomyocytes into a specific subtype after differentiation driven by the regulation of Wnt signaling. The medium used to optimize selection and maturation was in a glucose starvation conditions, supplemented with either a nutrition complex or ascorbic acid. Following optimized selection and maturation, more cardiac Troponin T (cTnT)-positive cardiomyocytes were detected using albumin and ascorbic acid than B27. In addition, ascorbic acid enriched maturation of ventricular cardiomyocytes. We compared cardiomyocyte-specific gene expression patterns under different selection and maturation conditions by next-generation sequencing (NGS) analysis. Our optimized conditions will enable simple and efficient maturation and specification of the desired cardiomyocyte subtype, facilitating both biomedical research and clinical applications. |
| first_indexed | 2024-03-08T15:57:38Z |
| format | Article |
| id | doaj.art-7f8a43b70e084a48bb598e863b7bf4d2 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-03-08T15:57:38Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-7f8a43b70e084a48bb598e863b7bf4d22024-01-08T17:12:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852023-12-01271839310.1080/19768354.2023.2189932Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation mediumJi-eun Kim0Eun-Mi Kim1Hyang-Ae Lee2Ki-Suk Kim3Dongguk University, Seoul, Republic of KoreaKorea Institute of Toxicology, Daejeon, Republic of KoreaKorea Institute of Toxicology, Daejeon, Republic of KoreaKorea Institute of Toxicology, Daejeon, Republic of KoreaABSTRACTCardiomyocytes derived from human pluripotent stem cells (hPSCs) can be used in various applications including disease modeling, drug safety screening, and novel cell-based cardiac therapies. Here, we report an optimized selection and maturation method to induce maturation of cardiomyocytes into a specific subtype after differentiation driven by the regulation of Wnt signaling. The medium used to optimize selection and maturation was in a glucose starvation conditions, supplemented with either a nutrition complex or ascorbic acid. Following optimized selection and maturation, more cardiac Troponin T (cTnT)-positive cardiomyocytes were detected using albumin and ascorbic acid than B27. In addition, ascorbic acid enriched maturation of ventricular cardiomyocytes. We compared cardiomyocyte-specific gene expression patterns under different selection and maturation conditions by next-generation sequencing (NGS) analysis. Our optimized conditions will enable simple and efficient maturation and specification of the desired cardiomyocyte subtype, facilitating both biomedical research and clinical applications.https://www.tandfonline.com/doi/10.1080/19768354.2023.2189932Cardiomyocyteascorbic acidalbuminWnt signaling |
| spellingShingle | Ji-eun Kim Eun-Mi Kim Hyang-Ae Lee Ki-Suk Kim Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium Animal Cells and Systems Cardiomyocyte ascorbic acid albumin Wnt signaling |
| title | Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium |
| title_full | Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium |
| title_fullStr | Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium |
| title_full_unstemmed | Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium |
| title_short | Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium |
| title_sort | effective derivation of ventricular cardiomyocytes from hpscs using ascorbic acid containing maturation medium |
| topic | Cardiomyocyte ascorbic acid albumin Wnt signaling |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2023.2189932 |
| work_keys_str_mv | AT jieunkim effectivederivationofventricularcardiomyocytesfromhpscsusingascorbicacidcontainingmaturationmedium AT eunmikim effectivederivationofventricularcardiomyocytesfromhpscsusingascorbicacidcontainingmaturationmedium AT hyangaelee effectivederivationofventricularcardiomyocytesfromhpscsusingascorbicacidcontainingmaturationmedium AT kisukkim effectivederivationofventricularcardiomyocytesfromhpscsusingascorbicacidcontainingmaturationmedium |