Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels
Roux-en-Y gastric bypass (RYGB) is an effective method to achieve sustained weight loss, but the mechanisms responsible for RYGB effects have not yet been fully characterized. In this study, we profiled the concentrations of 143 lipid metabolites in dry blood spots (DBS) of RYGB patients. DBS from o...
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MDPI AG
2018-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/8/4/83 |
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| author | Jarlei Fiamoncini Carina Fernandes Barbosa José Rubens Arnoni Junior José Celestino Araújo Junior Cinthia Taglieri Tiago Szego Barbara Gelhaus Heraldo Possolo de Souza Hannelore Daniel Thais Martins de Lima |
| author_facet | Jarlei Fiamoncini Carina Fernandes Barbosa José Rubens Arnoni Junior José Celestino Araújo Junior Cinthia Taglieri Tiago Szego Barbara Gelhaus Heraldo Possolo de Souza Hannelore Daniel Thais Martins de Lima |
| author_sort | Jarlei Fiamoncini |
| collection | DOAJ |
| description | Roux-en-Y gastric bypass (RYGB) is an effective method to achieve sustained weight loss, but the mechanisms responsible for RYGB effects have not yet been fully characterized. In this study, we profiled the concentrations of 143 lipid metabolites in dry blood spots (DBS) of RYGB patients. DBS from obese patients (BMI range 35–44 kg/m<sup>2</sup>) were collected 7 days before, 15 and 90 days after the surgery. LC-MS/MS was used to quantify acylcarnitines, phosphatidylcholines, sphingomyelins and bile acids. RYGB caused a rapid increase in acylcarnitine levels that proved to be only transient, contrasting with the sustained decrease in phosphatidylcholines and increase of sphingomyelins and bile acids. A PLS-DA analysis revealed a 3-component model (R<sup>2</sup> = 0.9, Q<sup>2</sup> = 0.74) with key metabolites responsible for the overall metabolite differences. These included the BCAA-derived acylcarnitines and sphingomyelins with 16 and 18 carbons. We found important correlations between the levels of BCAA-derived acylcarnitines and specific sphingomyelins with plasma cholesterol and triacylglycerol concentrations. Along with the marked weight loss and clinical improvements, RYGB induced specific alterations in plasma acylcarnitines, bile acid and phospholipid levels. This calls for more studies on RYGB effects aiming to elucidate the metabolic adaptations that follow this procedure. |
| first_indexed | 2024-12-11T17:51:59Z |
| format | Article |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-12-11T17:51:59Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7f8cbce1f40b44a3b50f31fe00d47cce2022-12-22T00:56:12ZengMDPI AGMetabolites2218-19892018-11-01848310.3390/metabo8040083metabo8040083Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid LevelsJarlei Fiamoncini0Carina Fernandes Barbosa1José Rubens Arnoni Junior2José Celestino Araújo Junior3Cinthia Taglieri4Tiago Szego5Barbara Gelhaus6Heraldo Possolo de Souza7Hannelore Daniel8Thais Martins de Lima9Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, 05508-060 São Paulo, BrazilClinica IMEC, Sao Paulo, 04260-020 São Paulo, BrazilClinica IMEC, Sao Paulo, 04260-020 São Paulo, BrazilClinica IMEC, Sao Paulo, 04260-020 São Paulo, BrazilClinica IMEC, Sao Paulo, 04260-020 São Paulo, BrazilInstituto CIGO, 05508-060 São Paulo, BrazilNutrition and Food Sciences, Technische Universität München, 85354 Freising-Weihenstephan, GermanyLaboratório de Emergências Clínicas (LIM 51), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 05508-060 São Paulo, BrazilNutrition and Food Sciences, Technische Universität München, 85354 Freising-Weihenstephan, GermanyLaboratório de Emergências Clínicas (LIM 51), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 05508-060 São Paulo, BrazilRoux-en-Y gastric bypass (RYGB) is an effective method to achieve sustained weight loss, but the mechanisms responsible for RYGB effects have not yet been fully characterized. In this study, we profiled the concentrations of 143 lipid metabolites in dry blood spots (DBS) of RYGB patients. DBS from obese patients (BMI range 35–44 kg/m<sup>2</sup>) were collected 7 days before, 15 and 90 days after the surgery. LC-MS/MS was used to quantify acylcarnitines, phosphatidylcholines, sphingomyelins and bile acids. RYGB caused a rapid increase in acylcarnitine levels that proved to be only transient, contrasting with the sustained decrease in phosphatidylcholines and increase of sphingomyelins and bile acids. A PLS-DA analysis revealed a 3-component model (R<sup>2</sup> = 0.9, Q<sup>2</sup> = 0.74) with key metabolites responsible for the overall metabolite differences. These included the BCAA-derived acylcarnitines and sphingomyelins with 16 and 18 carbons. We found important correlations between the levels of BCAA-derived acylcarnitines and specific sphingomyelins with plasma cholesterol and triacylglycerol concentrations. Along with the marked weight loss and clinical improvements, RYGB induced specific alterations in plasma acylcarnitines, bile acid and phospholipid levels. This calls for more studies on RYGB effects aiming to elucidate the metabolic adaptations that follow this procedure.https://www.mdpi.com/2218-1989/8/4/83obesitybariatric surgeryacylcarnitinebile acidsphospholipidsmetabolomics |
| spellingShingle | Jarlei Fiamoncini Carina Fernandes Barbosa José Rubens Arnoni Junior José Celestino Araújo Junior Cinthia Taglieri Tiago Szego Barbara Gelhaus Heraldo Possolo de Souza Hannelore Daniel Thais Martins de Lima Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels Metabolites obesity bariatric surgery acylcarnitine bile acids phospholipids metabolomics |
| title | Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels |
| title_full | Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels |
| title_fullStr | Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels |
| title_full_unstemmed | Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels |
| title_short | Roux-en-Y Gastric Bypass Surgery Induces Distinct but Frequently Transient Effects on Acylcarnitine, Bile Acid and Phospholipid Levels |
| title_sort | roux en y gastric bypass surgery induces distinct but frequently transient effects on acylcarnitine bile acid and phospholipid levels |
| topic | obesity bariatric surgery acylcarnitine bile acids phospholipids metabolomics |
| url | https://www.mdpi.com/2218-1989/8/4/83 |
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