Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury
Background: It has been reported that ferroptosis participates in the pathophysiological mechanism of spinal cord injury (SCI). Our preliminary experiments verified that dendrobium nobile polysaccharide (DNP) improved the behavioral function of SCI rats. Therefore, the purpose of this study was to e...
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IMR Press
2024-03-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/23/3/10.31083/j.jin2303065 |
| _version_ | 1797238624156123136 |
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| author | Jian Huang Jianghong Luo Ying Huang Linghong Wang Haibing Zhu Zhengnan Li Jianping Chen |
| author_facet | Jian Huang Jianghong Luo Ying Huang Linghong Wang Haibing Zhu Zhengnan Li Jianping Chen |
| author_sort | Jian Huang |
| collection | DOAJ |
| description | Background: It has been reported that ferroptosis participates in the pathophysiological mechanism of spinal cord injury (SCI). Our preliminary experiments verified that dendrobium nobile polysaccharide (DNP) improved the behavioral function of SCI rats. Therefore, the purpose of this study was to examine the role of DNP on ferroptosis and its neuroprotective mechanism in SCI rats. Methods: Adult female sprague dawley (SD) rats were exposed to SCI by Allen’s method, followed by an intragastric injection of 100 mg/kg DNP per day for 2 weeks. Behavioral features were verified by the Basso-Beattie-Bresnahan (BBB) scale and footprint evaluation. Iron content and glutathione (GSH) were assessed spectrophotometrically. Mitochondrial morphology was examined by transmission electron microscopy. The expression of ferroptosis-related genes, including System Xc- light chain (xCT), G-rich RNA sequence binding Factor 1 (GRSF1) and glutathione peroxidase 4 (Gpx4), was examined by real-time polymerase chain reaction (PCR) and western blot. The spinal cavity was defined using hematoxylin–eosin (HE) staining, and neuronal modifications were detected by immunofluorescence. Results: Compared with the SCI group, the BBB score of rats in the DNP group increased at 7 d, 14 d, 21 d, and 28 d. The differences between the two groups were statistically significant. At 12 h post-injury the iron content began to decrease. At 24 h post-injury the iron content decreased significantly in the DNP group. The morphological changes of the mitochondrial crest and membrane in the DNP group were ameliorated within 24 h. Compared with the sham group, the expression of xCT, GSH, Gpx4, and GRSF1 were significantly reduced after SCI. After DNP treatment, the expression of xCT, Gpx4, and GSH were higher. The tissue cavity area was significantly reduced and the amount of NeuN+ cells was increased in the DNP group at 14 d and 28 d after SCI. Conclusions: DNP facilitated the post-injury recovery in SCI rats via the inhibition of ferroptosis. |
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| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-04-24T17:38:36Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-7f8dae5bbaee464a8b2c33abe6fe1de72024-03-28T02:03:25ZengIMR PressJournal of Integrative Neuroscience0219-63522024-03-012336510.31083/j.jin2303065S0219-6352(23)00642-3Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord InjuryJian Huang0Jianghong Luo1Ying Huang2Linghong Wang3Haibing Zhu4Zhengnan Li5Jianping Chen6Department of Neurology, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, ChinaSchool of Public Health and Health Management, Gannan Medical University, 341000 Ganzhou, Jiangxi, ChinaDepartment of Neurology, Jinshan Hospital of Fudan University, 201508 Shanghai, ChinaDepartment of Neurology, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, ChinaDepartment of Neurology, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, ChinaDepartment of orthopaedics, Ganzhou People’s Hospital, 341000 Ganzhou, Jiangxi, ChinaDepartment of orthopaedics, Ganzhou People’s Hospital, 341000 Ganzhou, Jiangxi, ChinaBackground: It has been reported that ferroptosis participates in the pathophysiological mechanism of spinal cord injury (SCI). Our preliminary experiments verified that dendrobium nobile polysaccharide (DNP) improved the behavioral function of SCI rats. Therefore, the purpose of this study was to examine the role of DNP on ferroptosis and its neuroprotective mechanism in SCI rats. Methods: Adult female sprague dawley (SD) rats were exposed to SCI by Allen’s method, followed by an intragastric injection of 100 mg/kg DNP per day for 2 weeks. Behavioral features were verified by the Basso-Beattie-Bresnahan (BBB) scale and footprint evaluation. Iron content and glutathione (GSH) were assessed spectrophotometrically. Mitochondrial morphology was examined by transmission electron microscopy. The expression of ferroptosis-related genes, including System Xc- light chain (xCT), G-rich RNA sequence binding Factor 1 (GRSF1) and glutathione peroxidase 4 (Gpx4), was examined by real-time polymerase chain reaction (PCR) and western blot. The spinal cavity was defined using hematoxylin–eosin (HE) staining, and neuronal modifications were detected by immunofluorescence. Results: Compared with the SCI group, the BBB score of rats in the DNP group increased at 7 d, 14 d, 21 d, and 28 d. The differences between the two groups were statistically significant. At 12 h post-injury the iron content began to decrease. At 24 h post-injury the iron content decreased significantly in the DNP group. The morphological changes of the mitochondrial crest and membrane in the DNP group were ameliorated within 24 h. Compared with the sham group, the expression of xCT, GSH, Gpx4, and GRSF1 were significantly reduced after SCI. After DNP treatment, the expression of xCT, Gpx4, and GSH were higher. The tissue cavity area was significantly reduced and the amount of NeuN+ cells was increased in the DNP group at 14 d and 28 d after SCI. Conclusions: DNP facilitated the post-injury recovery in SCI rats via the inhibition of ferroptosis.https://www.imrpress.com/journal/JIN/23/3/10.31083/j.jin2303065spinal cord injurydendrobium nobile polysaccharidesferroptosisgpx4grsf1 |
| spellingShingle | Jian Huang Jianghong Luo Ying Huang Linghong Wang Haibing Zhu Zhengnan Li Jianping Chen Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury Journal of Integrative Neuroscience spinal cord injury dendrobium nobile polysaccharides ferroptosis gpx4 grsf1 |
| title | Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury |
| title_full | Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury |
| title_fullStr | Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury |
| title_full_unstemmed | Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury |
| title_short | Mechanism of Dendrobium Nobile Polysaccharide Inhibition of Ferroptosis in Rats with Spinal Cord Injury |
| title_sort | mechanism of dendrobium nobile polysaccharide inhibition of ferroptosis in rats with spinal cord injury |
| topic | spinal cord injury dendrobium nobile polysaccharides ferroptosis gpx4 grsf1 |
| url | https://www.imrpress.com/journal/JIN/23/3/10.31083/j.jin2303065 |
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