Serum and urine vitamin D metabolite analysis in early preeclampsia
Vitamin D deficiency is common in pregnant women and may contribute to adverse events in pregnancy such as preeclampsia (PET). To date, studies of vitamin D and PET have focused primarily on serum concentrations vitamin D, 25-hydroxyvitamin D3 (25(OH)D3) later in pregnancy. The aim here was to deter...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2018-01-01
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| Series: | Endocrine Connections |
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| Online Access: | http://www.endocrineconnections.com/content/7/1/199.full |
| _version_ | 1818932754244435968 |
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| author | J A Tamblyn C Jenkinson D P Larner M Hewison M D Kilby |
| author_facet | J A Tamblyn C Jenkinson D P Larner M Hewison M D Kilby |
| author_sort | J A Tamblyn |
| collection | DOAJ |
| description | Vitamin D deficiency is common in pregnant women and may contribute to adverse events in pregnancy such as preeclampsia (PET). To date, studies of vitamin D and PET have focused primarily on serum concentrations vitamin D, 25-hydroxyvitamin D3 (25(OH)D3) later in pregnancy. The aim here was to determine whether a more comprehensive analysis of vitamin D metabolites earlier in pregnancy could provide predictors of PET. Using samples from the SCOPE pregnancy cohort, multiple vitamin D metabolites were quantified by liquid chromatography–tandem mass spectrometry in paired serum and urine prior to the onset of PET symptoms. Samples from 50 women at pregnancy week 15 were analysed, with 25 (50%) developing PET by the end of the pregnancy and 25 continuing with uncomplicated pregnancy. Paired serum and urine from non-pregnant women (n = 9) of reproductive age were also used as a control. Serum concentrations of 25(OH)D3, 25(OH)D2, 1,25(OH)2D3, 24,25(OH)2D3 and
3-epi-25(OH)D3 were measured and showed no significant difference between women with uncomplicated pregnancies and those developing PET. As previously reported, serum 1,25(OH)2D3 was higher in all pregnant women (in the second trimester), but serum 25(OH)D2 was also higher compared to non-pregnant women. In urine, 25(OH)D3 and 24,25(OH)2D3 were quantifiable, with both metabolites demonstrating significantly lower (P < 0.05) concentrations of both of these metabolites in those destined to develop PET. These data indicate that analysis of urinary metabolites provides an additional insight into vitamin D and the kidney, with lower urinary 25(OH)D3 and 24,25(OH)2D3 excretion being an early indicator of a predisposition towards developing PET. |
| first_indexed | 2024-12-20T04:37:30Z |
| format | Article |
| id | doaj.art-7f8ecdd8ec8949eb980224489047a357 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-12-20T04:37:30Z |
| publishDate | 2018-01-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-7f8ecdd8ec8949eb980224489047a3572022-12-21T19:53:12ZengBioscientificaEndocrine Connections2049-36142049-36142018-01-017119921010.1530/EC-17-0308Serum and urine vitamin D metabolite analysis in early preeclampsiaJ A Tamblyn0C Jenkinson1D P Larner2M Hewison3M D Kilby4Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK; Birmingham Women’s Foundation Hospital, Edgbaston, Birmingham, UK; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UKBirmingham Women’s Foundation Hospital, Edgbaston, Birmingham, UKBirmingham Women’s Foundation Hospital, Edgbaston, Birmingham, UKInstitute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UKInstitute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK; Birmingham Women’s Foundation Hospital, Edgbaston, Birmingham, UK; Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UKVitamin D deficiency is common in pregnant women and may contribute to adverse events in pregnancy such as preeclampsia (PET). To date, studies of vitamin D and PET have focused primarily on serum concentrations vitamin D, 25-hydroxyvitamin D3 (25(OH)D3) later in pregnancy. The aim here was to determine whether a more comprehensive analysis of vitamin D metabolites earlier in pregnancy could provide predictors of PET. Using samples from the SCOPE pregnancy cohort, multiple vitamin D metabolites were quantified by liquid chromatography–tandem mass spectrometry in paired serum and urine prior to the onset of PET symptoms. Samples from 50 women at pregnancy week 15 were analysed, with 25 (50%) developing PET by the end of the pregnancy and 25 continuing with uncomplicated pregnancy. Paired serum and urine from non-pregnant women (n = 9) of reproductive age were also used as a control. Serum concentrations of 25(OH)D3, 25(OH)D2, 1,25(OH)2D3, 24,25(OH)2D3 and 3-epi-25(OH)D3 were measured and showed no significant difference between women with uncomplicated pregnancies and those developing PET. As previously reported, serum 1,25(OH)2D3 was higher in all pregnant women (in the second trimester), but serum 25(OH)D2 was also higher compared to non-pregnant women. In urine, 25(OH)D3 and 24,25(OH)2D3 were quantifiable, with both metabolites demonstrating significantly lower (P < 0.05) concentrations of both of these metabolites in those destined to develop PET. These data indicate that analysis of urinary metabolites provides an additional insight into vitamin D and the kidney, with lower urinary 25(OH)D3 and 24,25(OH)2D3 excretion being an early indicator of a predisposition towards developing PET.http://www.endocrineconnections.com/content/7/1/199.fullpregnancypreeclampsiavitamin Dbiomarkerserum and urine |
| spellingShingle | J A Tamblyn C Jenkinson D P Larner M Hewison M D Kilby Serum and urine vitamin D metabolite analysis in early preeclampsia Endocrine Connections pregnancy preeclampsia vitamin D biomarker serum and urine |
| title | Serum and urine vitamin D metabolite analysis in early preeclampsia |
| title_full | Serum and urine vitamin D metabolite analysis in early preeclampsia |
| title_fullStr | Serum and urine vitamin D metabolite analysis in early preeclampsia |
| title_full_unstemmed | Serum and urine vitamin D metabolite analysis in early preeclampsia |
| title_short | Serum and urine vitamin D metabolite analysis in early preeclampsia |
| title_sort | serum and urine vitamin d metabolite analysis in early preeclampsia |
| topic | pregnancy preeclampsia vitamin D biomarker serum and urine |
| url | http://www.endocrineconnections.com/content/7/1/199.full |
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