Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages
Puerarin, a class of isoflavonoid compounds concentrated in the roots of <i>Puerarias</i>, has antipyretic, sedative, and coronary blood-flow-increasing properties. Although the biosynthetic pathways of puerarin have been investigated by previous researchers, studies focusing on the infl...
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MDPI AG
2023-12-01
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| author | Xinyi Hu Ting Zhu Xinyi Min Jianing He Cong Hou Xia Liu |
| author_facet | Xinyi Hu Ting Zhu Xinyi Min Jianing He Cong Hou Xia Liu |
| author_sort | Xinyi Hu |
| collection | DOAJ |
| description | Puerarin, a class of isoflavonoid compounds concentrated in the roots of <i>Puerarias</i>, has antipyretic, sedative, and coronary blood-flow-increasing properties. Although the biosynthetic pathways of puerarin have been investigated by previous researchers, studies focusing on the influence of different growth stages on the accumulation of metabolites in the puerarin pathway are not detailed, and it is still controversial at the last step of the 8-<i>C</i>-glycosylation reaction. In this study, we conducted a comprehensive analysis of the metabolomic and transcriptomic changes in <i>Pueraria montana</i> var. thomsonii during two growing years, focusing on the vigorous growth and dormant stages, to elucidate the underlying mechanisms governing the changes in metabolite and gene expression within the puerarin biosynthesis pathway. In a comparison of the two growth stages in the two groups, puerarin and daidzin, the main downstream metabolites in the puerarin biosynthesis pathway, were found to accumulate mainly during the vigorous growth stage. We also identified 67 common differentially expressed genes in this pathway based on gene expression differences at different growth stages. Furthermore, we identified four candidate 8-<i>C</i>-GT genes that potentially contribute to the conversion of daidzein into puerarin and eight candidate 7-<i>O</i>-GT genes that may be involved in the conversion of daidzein into daidzin. A co-expression network analysis of important UGTs and HIDs along with daidzein and puerarin was conducted. Overall, our study contributes to the knowledge of puerarin biosynthesis and offers information about the stage at which the 8-<i>C</i>-glycosylation reaction occurs in biosynthesis. These findings provide valuable insights into the cultivation and quality enhancement of <i>Pueraria montana</i> var. thomsonii. |
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| language | English |
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| publishDate | 2023-12-01 |
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| series | Genes |
| spelling | doaj.art-7f916e9d0a0849099e3c122f52e599a82023-12-22T14:11:24ZengMDPI AGGenes2073-44252023-12-011412223010.3390/genes14122230Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth StagesXinyi Hu0Ting Zhu1Xinyi Min2Jianing He3Cong Hou4Xia Liu5School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaSchool of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, ChinaPuerarin, a class of isoflavonoid compounds concentrated in the roots of <i>Puerarias</i>, has antipyretic, sedative, and coronary blood-flow-increasing properties. Although the biosynthetic pathways of puerarin have been investigated by previous researchers, studies focusing on the influence of different growth stages on the accumulation of metabolites in the puerarin pathway are not detailed, and it is still controversial at the last step of the 8-<i>C</i>-glycosylation reaction. In this study, we conducted a comprehensive analysis of the metabolomic and transcriptomic changes in <i>Pueraria montana</i> var. thomsonii during two growing years, focusing on the vigorous growth and dormant stages, to elucidate the underlying mechanisms governing the changes in metabolite and gene expression within the puerarin biosynthesis pathway. In a comparison of the two growth stages in the two groups, puerarin and daidzin, the main downstream metabolites in the puerarin biosynthesis pathway, were found to accumulate mainly during the vigorous growth stage. We also identified 67 common differentially expressed genes in this pathway based on gene expression differences at different growth stages. Furthermore, we identified four candidate 8-<i>C</i>-GT genes that potentially contribute to the conversion of daidzein into puerarin and eight candidate 7-<i>O</i>-GT genes that may be involved in the conversion of daidzein into daidzin. A co-expression network analysis of important UGTs and HIDs along with daidzein and puerarin was conducted. Overall, our study contributes to the knowledge of puerarin biosynthesis and offers information about the stage at which the 8-<i>C</i>-glycosylation reaction occurs in biosynthesis. These findings provide valuable insights into the cultivation and quality enhancement of <i>Pueraria montana</i> var. thomsonii.https://www.mdpi.com/2073-4425/14/12/2230<i>Pueraria montana</i> var. thomsoniipuerarin biosynthesistranscriptomemetabolome |
| spellingShingle | Xinyi Hu Ting Zhu Xinyi Min Jianing He Cong Hou Xia Liu Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages Genes <i>Pueraria montana</i> var. thomsonii puerarin biosynthesis transcriptome metabolome |
| title | Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages |
| title_full | Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages |
| title_fullStr | Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages |
| title_full_unstemmed | Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages |
| title_short | Integrated Metabolomic and Transcriptomic Analysis of Puerarin Biosynthesis in <i>Pueraria montana</i> var. thomsonii at Different Growth Stages |
| title_sort | integrated metabolomic and transcriptomic analysis of puerarin biosynthesis in i pueraria montana i var thomsonii at different growth stages |
| topic | <i>Pueraria montana</i> var. thomsonii puerarin biosynthesis transcriptome metabolome |
| url | https://www.mdpi.com/2073-4425/14/12/2230 |
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