Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?
The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from h...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/5/2830 |
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| author | Cristina Bucchi Ella Ohlsson Josep Maria de Anta Melanie Woelflick Kerstin Galler María Cristina Manzanares-Cespedes Matthias Widbiller |
| author_facet | Cristina Bucchi Ella Ohlsson Josep Maria de Anta Melanie Woelflick Kerstin Galler María Cristina Manzanares-Cespedes Matthias Widbiller |
| author_sort | Cristina Bucchi |
| collection | DOAJ |
| description | The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial–mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial–mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition. |
| first_indexed | 2024-03-09T20:36:03Z |
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| id | doaj.art-7f9229a166744b33b6c4e9aadd55e4b5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:36:03Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7f9229a166744b33b6c4e9aadd55e4b52023-11-23T23:10:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01235283010.3390/ijms23052830Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?Cristina Bucchi0Ella Ohlsson1Josep Maria de Anta2Melanie Woelflick3Kerstin Galler4María Cristina Manzanares-Cespedes5Matthias Widbiller6Research Centre for Dental Sciences (CICO), Department of Integral Adult Dentistry, Faculty of Dentistry, Universidad de La Frontera, Temuco 4811230, ChileDepartment of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, GermanyHuman Anatomy and Embryology Unit, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, 08907 L’Hospitalet de Llobregat, SpainDepartment of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Conservative Dentistry and Periodontology, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, GermanyHuman Anatomy and Embryology Unit, Department of Pathology and Experimental Therapeutics, Faculty of Medicine and Health Sciences, Campus de Bellvitge, Universitat de Barcelona, 08907 L’Hospitalet de Llobregat, SpainDepartment of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93053 Regensburg, GermanyThe aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial–mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial–mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.https://www.mdpi.com/1422-0067/23/5/2830human amnion epithelial cellsdental pulp stem cellsdentin matrix proteinsodontoblastic differentiationrevitalization |
| spellingShingle | Cristina Bucchi Ella Ohlsson Josep Maria de Anta Melanie Woelflick Kerstin Galler María Cristina Manzanares-Cespedes Matthias Widbiller Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? International Journal of Molecular Sciences human amnion epithelial cells dental pulp stem cells dentin matrix proteins odontoblastic differentiation revitalization |
| title | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
| title_full | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
| title_fullStr | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
| title_full_unstemmed | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
| title_short | Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? |
| title_sort | human amnion epithelial cells a potential cell source for pulp regeneration |
| topic | human amnion epithelial cells dental pulp stem cells dentin matrix proteins odontoblastic differentiation revitalization |
| url | https://www.mdpi.com/1422-0067/23/5/2830 |
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