Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways
BackgroundBoth melatonin and indole-3-acetic acid (IAA) are derived from tryptophan. And the most interesting and unsolved puzzle in melatonin research is that what is the relationship between melatonin and auxin?MethodsIn this study, we performed transcriptome analysis with a time series method to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1057993/full |
| _version_ | 1811187455992266752 |
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| author | Wei Wei Jian-Jun Tao Cui-Cui Yin Shou-Yi Chen Jin-Song Zhang Jin-Song Zhang Wan-Ke Zhang |
| author_facet | Wei Wei Jian-Jun Tao Cui-Cui Yin Shou-Yi Chen Jin-Song Zhang Jin-Song Zhang Wan-Ke Zhang |
| author_sort | Wei Wei |
| collection | DOAJ |
| description | BackgroundBoth melatonin and indole-3-acetic acid (IAA) are derived from tryptophan. And the most interesting and unsolved puzzle in melatonin research is that what is the relationship between melatonin and auxin?MethodsIn this study, we performed transcriptome analysis with a time series method to disclose the connection of the two metabolites in soybean.ResultsOur results reveal that melatonin and IAA treatments cause substantial overlaps in gene expression changes. Common genes of melatonin and IAA treatments could be sorted into clusters with very similar expression tendency. A KEGG assay showed that exogenous applied melatonin enriched differentially expressed genes in auxin biosynthesis and signaling pathways. For details, melatonin up-regulates several YUCCA genes which participate in auxin biosynthesis; melatonin also enhances expression levels of auxin receptor coding genes, such as TIR1, AFB3 and AFB5; dozens of genes involved in auxin transport, such as AUXI and PIN, are regulated by melatonin similarly as by auxin; auxin-responsive genes, such as IAA, ARF, GH3 and SAUR-like genes, intensively respond to melatonin as well as to auxin. A DR5 promoter mediated GUS staining assay showed that low concentration of melatonin could induce auxin biosynthesis in a dosage manner, whereas high concentration of melatonin would eliminate such effect. At last, gene ontology (GO) analysis suggests that melatonin treatment has similar characteristics as auxin treatment in many processes. However, the two molecules still keep their own features respectively. For example, melatonin takes part in stress responses, while IAA treatment enriches the GO terms that related to cell growth.ConclusionTaken together, exogenous applied melatonin, if not exceeds the appropriate concentration, could promote auxin responses range from biosynthesis to signaling transduction. Thus, our research is a key part to explain the auxin-like roles of melatonin in regulating plant growth. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T14:03:47Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7f97b61b2f6849e48b42c7a5028ff27e2022-12-22T04:19:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10579931057993Melatonin regulates gene expressions through activating auxin synthesis and signaling pathwaysWei Wei0Jian-Jun Tao1Cui-Cui Yin2Shou-Yi Chen3Jin-Song Zhang4Jin-Song Zhang5Wan-Ke Zhang6State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaState Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaState Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaState Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaState Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, ChinaBackgroundBoth melatonin and indole-3-acetic acid (IAA) are derived from tryptophan. And the most interesting and unsolved puzzle in melatonin research is that what is the relationship between melatonin and auxin?MethodsIn this study, we performed transcriptome analysis with a time series method to disclose the connection of the two metabolites in soybean.ResultsOur results reveal that melatonin and IAA treatments cause substantial overlaps in gene expression changes. Common genes of melatonin and IAA treatments could be sorted into clusters with very similar expression tendency. A KEGG assay showed that exogenous applied melatonin enriched differentially expressed genes in auxin biosynthesis and signaling pathways. For details, melatonin up-regulates several YUCCA genes which participate in auxin biosynthesis; melatonin also enhances expression levels of auxin receptor coding genes, such as TIR1, AFB3 and AFB5; dozens of genes involved in auxin transport, such as AUXI and PIN, are regulated by melatonin similarly as by auxin; auxin-responsive genes, such as IAA, ARF, GH3 and SAUR-like genes, intensively respond to melatonin as well as to auxin. A DR5 promoter mediated GUS staining assay showed that low concentration of melatonin could induce auxin biosynthesis in a dosage manner, whereas high concentration of melatonin would eliminate such effect. At last, gene ontology (GO) analysis suggests that melatonin treatment has similar characteristics as auxin treatment in many processes. However, the two molecules still keep their own features respectively. For example, melatonin takes part in stress responses, while IAA treatment enriches the GO terms that related to cell growth.ConclusionTaken together, exogenous applied melatonin, if not exceeds the appropriate concentration, could promote auxin responses range from biosynthesis to signaling transduction. Thus, our research is a key part to explain the auxin-like roles of melatonin in regulating plant growth.https://www.frontiersin.org/articles/10.3389/fpls.2022.1057993/fullmelatoninauxinsoybeantranscriptometime series analysis |
| spellingShingle | Wei Wei Jian-Jun Tao Cui-Cui Yin Shou-Yi Chen Jin-Song Zhang Jin-Song Zhang Wan-Ke Zhang Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways Frontiers in Plant Science melatonin auxin soybean transcriptome time series analysis |
| title | Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| title_full | Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| title_fullStr | Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| title_full_unstemmed | Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| title_short | Melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| title_sort | melatonin regulates gene expressions through activating auxin synthesis and signaling pathways |
| topic | melatonin auxin soybean transcriptome time series analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1057993/full |
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