The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis
Muscle development is a complex biological process involving an intricate network of multiple factor interactions. Through the analysis of transcriptome data and molecular biology confirmation, this study aims to reveal the molecular mechanism underlying sheep embryonic skeletal muscle development....
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/6/943 |
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| author | Wenping Hu Xinyue Wang Yazhen Bi Jingjing Bao Mingyu Shang Li Zhang |
| author_facet | Wenping Hu Xinyue Wang Yazhen Bi Jingjing Bao Mingyu Shang Li Zhang |
| author_sort | Wenping Hu |
| collection | DOAJ |
| description | Muscle development is a complex biological process involving an intricate network of multiple factor interactions. Through the analysis of transcriptome data and molecular biology confirmation, this study aims to reveal the molecular mechanism underlying sheep embryonic skeletal muscle development. The RNA sequencing of embryos was conducted, and microRNA (miRNA)-mediated competitive endogenous RNA (ceRNA) networks were constructed. qRT-PCR, siRNA knockdown, CCK-8 assay, scratch assay, and dual luciferase assay were used to carry out gene function identification. Through the analysis of the ceRNA networks, three miRNAs (miR-493-3p, miR-3959-3p, and miR-410-5p) and three genes (<i>TEAD1</i>, <i>ZBTB34,</i> and <i>POGLUT1</i>) were identified. The qRT-PCR of the DE-miRNAs and genes in the muscle tissues of sheep showed that the expression levels of the <i>TEAD1</i> gene and miR-410-5p were correlated with the growth rate. The knockdown of the <i>TEAD1</i> gene by siRNA could significantly inhibit the proliferation of sheep primary embryonic myoblasts, and the expression levels of <i>SLC1A5, FoxO3, MyoD</i>, and <i>Pax7</i> were significantly downregulated. The targeting relationship between miR-410-5p and the <i>TEAD1</i> gene was validated by a dual luciferase assay, and miR-410-5p can significantly downregulate the expression of <i>TEAD1</i> in sheep primary embryonic myoblasts. We proved the regulatory relationship between miR-410-5p and the <i>TEAD1</i> gene, which was related to the proliferation of sheep embryonic myoblasts. The results provide a reference and molecular basis for understanding the molecular mechanism of embryonic muscle development. |
| first_indexed | 2024-03-11T06:47:42Z |
| format | Article |
| id | doaj.art-7f9858fcb28647aba2740e92d01e2168 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T06:47:42Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-7f9858fcb28647aba2740e92d01e21682023-11-17T10:14:03ZengMDPI AGCells2073-44092023-03-0112694310.3390/cells12060943The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network AnalysisWenping Hu0Xinyue Wang1Yazhen Bi2Jingjing Bao3Mingyu Shang4Li Zhang5Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaMuscle development is a complex biological process involving an intricate network of multiple factor interactions. Through the analysis of transcriptome data and molecular biology confirmation, this study aims to reveal the molecular mechanism underlying sheep embryonic skeletal muscle development. The RNA sequencing of embryos was conducted, and microRNA (miRNA)-mediated competitive endogenous RNA (ceRNA) networks were constructed. qRT-PCR, siRNA knockdown, CCK-8 assay, scratch assay, and dual luciferase assay were used to carry out gene function identification. Through the analysis of the ceRNA networks, three miRNAs (miR-493-3p, miR-3959-3p, and miR-410-5p) and three genes (<i>TEAD1</i>, <i>ZBTB34,</i> and <i>POGLUT1</i>) were identified. The qRT-PCR of the DE-miRNAs and genes in the muscle tissues of sheep showed that the expression levels of the <i>TEAD1</i> gene and miR-410-5p were correlated with the growth rate. The knockdown of the <i>TEAD1</i> gene by siRNA could significantly inhibit the proliferation of sheep primary embryonic myoblasts, and the expression levels of <i>SLC1A5, FoxO3, MyoD</i>, and <i>Pax7</i> were significantly downregulated. The targeting relationship between miR-410-5p and the <i>TEAD1</i> gene was validated by a dual luciferase assay, and miR-410-5p can significantly downregulate the expression of <i>TEAD1</i> in sheep primary embryonic myoblasts. We proved the regulatory relationship between miR-410-5p and the <i>TEAD1</i> gene, which was related to the proliferation of sheep embryonic myoblasts. The results provide a reference and molecular basis for understanding the molecular mechanism of embryonic muscle development.https://www.mdpi.com/2073-4409/12/6/943ceRNA networkskeletal musclesheep<i>TEAD1</i> genemiR-410-5p |
| spellingShingle | Wenping Hu Xinyue Wang Yazhen Bi Jingjing Bao Mingyu Shang Li Zhang The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis Cells ceRNA network skeletal muscle sheep <i>TEAD1</i> gene miR-410-5p |
| title | The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis |
| title_full | The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis |
| title_fullStr | The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis |
| title_full_unstemmed | The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis |
| title_short | The Molecular Mechanism of the TEAD1 Gene and miR-410-5p Affect Embryonic Skeletal Muscle Development: A miRNA-Mediated ceRNA Network Analysis |
| title_sort | molecular mechanism of the tead1 gene and mir 410 5p affect embryonic skeletal muscle development a mirna mediated cerna network analysis |
| topic | ceRNA network skeletal muscle sheep <i>TEAD1</i> gene miR-410-5p |
| url | https://www.mdpi.com/2073-4409/12/6/943 |
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