Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern
Diarrhea is a prevalent and economically impactful ailment in cattle herds, resulting in substantial losses attributed to mortality, treatment expenses, and impaired calf growth. The aim of this study was to establish data about the occurrence of the main bacterial agents involved in diarrhea in dai...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2024-03-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acve-2024-0008 |
| _version_ | 1797258384121004032 |
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| author | Trindade Gonçalves Lucilene Martins Santos Caroline Lima Policarpo Wendel Adelino de Melo Santana Lisa Hauane Medeiros de Oliveira Kely Janine Bezerra Ana Catarina Pinheiro Angelim Ribeiro Sousa Jaize Viana Araújo Piancó Luísa Silva Diego Marques Costa Veiga de Sousa Leandro Henrique de Oliveira Santos Giselle Cutrim Barroso de Carvalho Robert Ferreira Azevedo Carvalho Isabel Pereira Santos Hamilton de Moraes Pereira Helder |
| author_facet | Trindade Gonçalves Lucilene Martins Santos Caroline Lima Policarpo Wendel Adelino de Melo Santana Lisa Hauane Medeiros de Oliveira Kely Janine Bezerra Ana Catarina Pinheiro Angelim Ribeiro Sousa Jaize Viana Araújo Piancó Luísa Silva Diego Marques Costa Veiga de Sousa Leandro Henrique de Oliveira Santos Giselle Cutrim Barroso de Carvalho Robert Ferreira Azevedo Carvalho Isabel Pereira Santos Hamilton de Moraes Pereira Helder |
| author_sort | Trindade Gonçalves Lucilene Martins |
| collection | DOAJ |
| description | Diarrhea is a prevalent and economically impactful ailment in cattle herds, resulting in substantial losses attributed to mortality, treatment expenses, and impaired calf growth. The aim of this study was to establish data about the occurrence of the main bacterial agents involved in diarrhea in dairy calves in the state of Maranhão, as well as to determine the most effective and/or resistant antibiotics, establishing a foundation for treatment and prevention protocols. The samples were collected with rectal swabs and initially cultivated on sheep blood agar (5%) and MacConkey agar and later on Salmonella-Shigella agar and eosin-methylene blue agar. Gram staining, biochemical tests, and antibiograms were performed on the obtained colonies. Ten municipalities were studied, with 230 calves evaluated from ten dairy farms. A total of 21 animals exhibited clinical signs of diarrhea, representing a frequency of 9.13%. The frequency of positive farms was 35%, and in 50% of municipalities. Bacteria isolated belonged to the Enterobacteriaceae family, with 71.4% Escherichia coli, 14.3% Enterobacter sp., 9.5% Proteus sp. and 4.8% Klebsiella sp. On the antibiogram, the bacteria that showed the highest resistance levels were those of the Proteus genus, followed by Enterobacter sp. and E. coli. The bacteria found are relevant for both animal and human health due to their zoonotic potential and serve as a public health alert since the isolates in this study showed in vitro resistance to several antibiotics, which predisposes them to the emergence of multidrug-resistant bacteria. |
| first_indexed | 2024-04-24T22:52:40Z |
| format | Article |
| id | doaj.art-7f9a5c94cb1e493199c2747cb6f053aa |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-04-24T22:52:40Z |
| publishDate | 2024-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-7f9a5c94cb1e493199c2747cb6f053aa2024-03-18T10:28:46ZengSciendoActa Veterinaria1820-74482024-03-0174110611610.2478/acve-2024-0008Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance PatternTrindade Gonçalves Lucilene Martins0Santos Caroline Lima1Policarpo Wendel Adelino2de Melo Santana Lisa Hauane3Medeiros de Oliveira Kely Janine4Bezerra Ana Catarina Pinheiro Angelim5Ribeiro Sousa Jaize Viana6Araújo Piancó Luísa7Silva Diego Marques Costa8Veiga de Sousa Leandro Henrique9de Oliveira Santos Giselle Cutrim10Barroso de Carvalho Robert Ferreira11Azevedo Carvalho Isabel12Pereira Santos Hamilton13de Moraes Pereira Helder14State University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilAgricultural Defense Agency of Maranhao (AGED-MA), Pedreiras, MA, BrazilState University of Maranhão, Laboratory of Food and Water Microbiology, São Luís, MA, BrazilState University of Maranhão, Laboratory of Infectious Disease Diagnostic São Luís, MA, BrazilState University of Maranhão, Laboratory of Clinical Veterinary Bacteriology, São Luís, MA, BrazilDiarrhea is a prevalent and economically impactful ailment in cattle herds, resulting in substantial losses attributed to mortality, treatment expenses, and impaired calf growth. The aim of this study was to establish data about the occurrence of the main bacterial agents involved in diarrhea in dairy calves in the state of Maranhão, as well as to determine the most effective and/or resistant antibiotics, establishing a foundation for treatment and prevention protocols. The samples were collected with rectal swabs and initially cultivated on sheep blood agar (5%) and MacConkey agar and later on Salmonella-Shigella agar and eosin-methylene blue agar. Gram staining, biochemical tests, and antibiograms were performed on the obtained colonies. Ten municipalities were studied, with 230 calves evaluated from ten dairy farms. A total of 21 animals exhibited clinical signs of diarrhea, representing a frequency of 9.13%. The frequency of positive farms was 35%, and in 50% of municipalities. Bacteria isolated belonged to the Enterobacteriaceae family, with 71.4% Escherichia coli, 14.3% Enterobacter sp., 9.5% Proteus sp. and 4.8% Klebsiella sp. On the antibiogram, the bacteria that showed the highest resistance levels were those of the Proteus genus, followed by Enterobacter sp. and E. coli. The bacteria found are relevant for both animal and human health due to their zoonotic potential and serve as a public health alert since the isolates in this study showed in vitro resistance to several antibiotics, which predisposes them to the emergence of multidrug-resistant bacteria.https://doi.org/10.2478/acve-2024-0008diarrheadairy farmsenterobacteriaceaeantibiogrammultidrug resistance. |
| spellingShingle | Trindade Gonçalves Lucilene Martins Santos Caroline Lima Policarpo Wendel Adelino de Melo Santana Lisa Hauane Medeiros de Oliveira Kely Janine Bezerra Ana Catarina Pinheiro Angelim Ribeiro Sousa Jaize Viana Araújo Piancó Luísa Silva Diego Marques Costa Veiga de Sousa Leandro Henrique de Oliveira Santos Giselle Cutrim Barroso de Carvalho Robert Ferreira Azevedo Carvalho Isabel Pereira Santos Hamilton de Moraes Pereira Helder Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern Acta Veterinaria diarrhea dairy farms enterobacteriaceae antibiogram multidrug resistance. |
| title | Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern |
| title_full | Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern |
| title_fullStr | Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern |
| title_full_unstemmed | Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern |
| title_short | Enterobacteria Isolated From Diarrheic Calves and their Phenotypic Resistance Pattern |
| title_sort | enterobacteria isolated from diarrheic calves and their phenotypic resistance pattern |
| topic | diarrhea dairy farms enterobacteriaceae antibiogram multidrug resistance. |
| url | https://doi.org/10.2478/acve-2024-0008 |
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