Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles
(1) Background: Cortisol and melatonin (MT) act in regulating follicular development. We hypothesized that abnormal levels of cortisol, MT, and steroids in theca interna cells might be involved in the development of follicular cysts in sows. (2) Methods: To test this hypothesis, we measured the mRNA...
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MDPI AG
2022-02-01
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| author | Yusheng Qin Jiahua Bai Jiage Dai Jianhui Zhou Taipeng Zhang Silong Zhang Xiaoling Xu Yan Liu |
| author_facet | Yusheng Qin Jiahua Bai Jiage Dai Jianhui Zhou Taipeng Zhang Silong Zhang Xiaoling Xu Yan Liu |
| author_sort | Yusheng Qin |
| collection | DOAJ |
| description | (1) Background: Cortisol and melatonin (MT) act in regulating follicular development. We hypothesized that abnormal levels of cortisol, MT, and steroids in theca interna cells might be involved in the development of follicular cysts in sows. (2) Methods: To test this hypothesis, we measured the mRNA levels of enzymes involved in steroid hormone synthesis, the glucocorticoid receptor (GR), and melatonin receptors (MTRs) in theca interna cells of cystic and normal porcine follicles. (3) Results: The concentrations of estradiol, progesterone, and cortisol were greater in cystic follicles than in control ones (<i>p</i> = 0.034, <i>p</i> = 0.020, <i>p</i> = 0.000), but the concentration of MT was significantly lower (<i>p</i> = 0.045). The levels of <i>GR, 11β-HSD1</i>, and <i>11β-HSD2</i> were higher in cystic follicles than in control l follicles. MT types 1 and 2 were significantly lower in cystic follicles (<i>p</i> < 0.05). The mRNA expression levels of genes encoding the steroid hormone synthesis enzymes, steroidogenic acute regulatory protein (<i>StAR</i>), recombinant cytochrome <i>P45011A1</i> (<i>CYP11A1</i>), and 3β-hydroxysteroid dehydrogenase (<i>3β-HSD</i>) in theca interna cells of cystic follicles were significantly higher than in control follicles. Thus, there was disruption of hormone secretion in the fluid of cystic follicles in sows. (4) Conclusions: The levels of steroid hormones, cortisol and MT are disrupted in porcine cystic follicles. |
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| spelling | doaj.art-7f9f5205a1ba44e09632569f03a1748f2023-11-23T15:48:31ZengMDPI AGAnimals2076-26152022-02-0112335710.3390/ani12030357Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian FolliclesYusheng Qin0Jiahua Bai1Jiage Dai2Jianhui Zhou3Taipeng Zhang4Silong Zhang5Xiaoling Xu6Yan Liu7Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaGuilin Animal Husbandry Station, Guilin 541000, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China(1) Background: Cortisol and melatonin (MT) act in regulating follicular development. We hypothesized that abnormal levels of cortisol, MT, and steroids in theca interna cells might be involved in the development of follicular cysts in sows. (2) Methods: To test this hypothesis, we measured the mRNA levels of enzymes involved in steroid hormone synthesis, the glucocorticoid receptor (GR), and melatonin receptors (MTRs) in theca interna cells of cystic and normal porcine follicles. (3) Results: The concentrations of estradiol, progesterone, and cortisol were greater in cystic follicles than in control ones (<i>p</i> = 0.034, <i>p</i> = 0.020, <i>p</i> = 0.000), but the concentration of MT was significantly lower (<i>p</i> = 0.045). The levels of <i>GR, 11β-HSD1</i>, and <i>11β-HSD2</i> were higher in cystic follicles than in control l follicles. MT types 1 and 2 were significantly lower in cystic follicles (<i>p</i> < 0.05). The mRNA expression levels of genes encoding the steroid hormone synthesis enzymes, steroidogenic acute regulatory protein (<i>StAR</i>), recombinant cytochrome <i>P45011A1</i> (<i>CYP11A1</i>), and 3β-hydroxysteroid dehydrogenase (<i>3β-HSD</i>) in theca interna cells of cystic follicles were significantly higher than in control follicles. Thus, there was disruption of hormone secretion in the fluid of cystic follicles in sows. (4) Conclusions: The levels of steroid hormones, cortisol and MT are disrupted in porcine cystic follicles.https://www.mdpi.com/2076-2615/12/3/357pigfollicular cystcortisolmelatoninsteroid hormones |
| spellingShingle | Yusheng Qin Jiahua Bai Jiage Dai Jianhui Zhou Taipeng Zhang Silong Zhang Xiaoling Xu Yan Liu Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles Animals pig follicular cyst cortisol melatonin steroid hormones |
| title | Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles |
| title_full | Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles |
| title_fullStr | Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles |
| title_full_unstemmed | Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles |
| title_short | Alterations of Cortisol and Melatonin Production by the Theca Interna Cells of Porcine Cystic Ovarian Follicles |
| title_sort | alterations of cortisol and melatonin production by the theca interna cells of porcine cystic ovarian follicles |
| topic | pig follicular cyst cortisol melatonin steroid hormones |
| url | https://www.mdpi.com/2076-2615/12/3/357 |
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