| Summary: | Twilight zones in oceans represent the oceanic waters between 200 m to 1000 m in depth, wherein sunlight is diffused and intensity is <1% of surface value. The activities and diversity of marine micro-organisms in this unique zone are understudied, especially in the Indian Sector of the Southern Ocean. For a better understanding of the microbial environment and diversity in the twilight zone of the Indian sector of Southern Ocean, samples were collected from 200m depth in eddy-influenced waters of Subtropical Front (STF), Sub-Antarctic Front (SAF), Polar Front (PF), waters off Kerguelen (Kw), and Prydz Bay (Pb) waters. In this article, next-generation sequencing (NGS) based amplicon data of 16s rDNA bacterial samples are presented. Hypervariable V3-V4 regions were sequenced using Hiseq platform, and data was processed using Mothur v 1.48.0, and database Silva 138.1nr. Total of nine different phyla is reported from the Southern Ocean at 200m, whereas at order level Synechococcales was found in STF waters only and SAR 11_ Clades were present in all stations.
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