Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes
The deficiency of vitamin D is associated with the risk of various chronic diseases, including diabetes mellitus and its complications. Given the strong genomic action of vitamin D hormone-active form, its deficiency can lead to dysfunction of cytokine signaling pathways, including those dependent o...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-08-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Labudzynskyi_4_20.pdf |
| _version_ | 1827802058201235456 |
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| author | D. O. Labudzynskyi I. O. Shymanskyi O. O. Lisakovska A. O. Mazanova L. V. Natrus M. M. Veliky |
| author_facet | D. O. Labudzynskyi I. O. Shymanskyi O. O. Lisakovska A. O. Mazanova L. V. Natrus M. M. Veliky |
| author_sort | D. O. Labudzynskyi |
| collection | DOAJ |
| description | The deficiency of vitamin D is associated with the risk of various chronic diseases, including diabetes mellitus and its complications. Given the strong genomic action of vitamin D hormone-active form, its deficiency can lead to dysfunction of cytokine signaling pathways, including those dependent on vascular endothelial growth factors (VEGFs) and apelin. The present study was carried out to define the link between VEGF-A and apelin expression in liver, hepatocytes viability and vitamin D status at experimental type 1 diabetes in mice. We established that chronic hyperglycemia at streptozotocin-induced diabetes was accompanied by a 2.2-fold decrease in 25OHD content in the serum and increased hepatocytes apoptosis and necrosis. Vitamin D deficiency correlated with increased apelin and VEGF-A (8- and 1.6-fold respectively) expression. Almost complete restoration of circulatory 25OHD content in serum was achieved at vitamin D3 treatment (800 IU/kg, per os, for 2 months) followed by reduced apelin and VEGF-A expression in liver and the decline of hepatocytes apoptosis. We conclude that vitamin D3 can be involved in cell survival, angiogenesis and fibrogenesis by modulating VEGF-A and apelin dependent regulatory systems in diabetic liver. |
| first_indexed | 2024-03-11T20:34:24Z |
| format | Article |
| id | doaj.art-7fa2cbedc842455a8624771e5a37ab5f |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T20:34:24Z |
| publishDate | 2020-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-7fa2cbedc842455a8624771e5a37ab5f2023-10-02T07:23:03ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032020-08-0192451310.15407/ubj92.04.005Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetesD. O. Labudzynskyi0https://orcid.org/0000-0003-4389-6049I. O. Shymanskyi1https://orcid.org/0000-0002-1507-8906O. O. Lisakovska2https://orcid.org/0000-0002-5844-1453A. O. Mazanova3https://orcid.org/0000-0002-3211-5094L. V. Natrus4https://orcid.org/0000-0003-1763-0618M. M. Veliky5Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivBogomolets National Medical University, Kyiv, UkrainePalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivThe deficiency of vitamin D is associated with the risk of various chronic diseases, including diabetes mellitus and its complications. Given the strong genomic action of vitamin D hormone-active form, its deficiency can lead to dysfunction of cytokine signaling pathways, including those dependent on vascular endothelial growth factors (VEGFs) and apelin. The present study was carried out to define the link between VEGF-A and apelin expression in liver, hepatocytes viability and vitamin D status at experimental type 1 diabetes in mice. We established that chronic hyperglycemia at streptozotocin-induced diabetes was accompanied by a 2.2-fold decrease in 25OHD content in the serum and increased hepatocytes apoptosis and necrosis. Vitamin D deficiency correlated with increased apelin and VEGF-A (8- and 1.6-fold respectively) expression. Almost complete restoration of circulatory 25OHD content in serum was achieved at vitamin D3 treatment (800 IU/kg, per os, for 2 months) followed by reduced apelin and VEGF-A expression in liver and the decline of hepatocytes apoptosis. We conclude that vitamin D3 can be involved in cell survival, angiogenesis and fibrogenesis by modulating VEGF-A and apelin dependent regulatory systems in diabetic liver.http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Labudzynskyi_4_20.pdfangiogenesisapelinapoptosisexperimental type 1 diabeteslivervegfvitamin d3 |
| spellingShingle | D. O. Labudzynskyi I. O. Shymanskyi O. O. Lisakovska A. O. Mazanova L. V. Natrus M. M. Veliky Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes The Ukrainian Biochemical Journal angiogenesis apelin apoptosis experimental type 1 diabetes liver vegf vitamin d3 |
| title | Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes |
| title_full | Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes |
| title_fullStr | Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes |
| title_full_unstemmed | Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes |
| title_short | Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes |
| title_sort | vitamin d 3 regulates hepatic vegf a and apelin expression in experimental type 1 diabetes |
| topic | angiogenesis apelin apoptosis experimental type 1 diabetes liver vegf vitamin d3 |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2020/09/Labudzynskyi_4_20.pdf |
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