Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice
We describe here a simple, cost-effective apparatus for continuous tethered electroencephalographic (EEG) monitoring of spontaneous recurrent seizures in mice. We used a small, low torque slip ring as an EEG commutator, mounted the slip ring onto a standard mouse cage and connected rotary wires of t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00172/full |
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| author | Na-Ryum Bin Na-Ryum Bin Hongmei Song Hongmei Song Chiping Wu Marcus Lau Shuzo Sugita Shuzo Sugita James H. Eubanks James H. Eubanks James H. Eubanks James H. Eubanks Liang Zhang Liang Zhang Liang Zhang |
| author_facet | Na-Ryum Bin Na-Ryum Bin Hongmei Song Hongmei Song Chiping Wu Marcus Lau Shuzo Sugita Shuzo Sugita James H. Eubanks James H. Eubanks James H. Eubanks James H. Eubanks Liang Zhang Liang Zhang Liang Zhang |
| author_sort | Na-Ryum Bin |
| collection | DOAJ |
| description | We describe here a simple, cost-effective apparatus for continuous tethered electroencephalographic (EEG) monitoring of spontaneous recurrent seizures in mice. We used a small, low torque slip ring as an EEG commutator, mounted the slip ring onto a standard mouse cage and connected rotary wires of the slip ring directly to animal's implanted headset. Modifications were made in the cage to allow for a convenient installation of the slip ring and accommodation of animal ambient activity. We tested the apparatus for hippocampal EEG recordings in adult C57 black mice. Spontaneous recurrent seizures were induced using extended hippocampal kindling (≥95 daily stimulation). Control animals underwent similar hippocampal electrode implantations but no stimulations were given. Combined EEG and webcam monitoring were performed for 24 h daily for 5–9 consecutive days. During the monitoring periods, the animals moved and accessed water and food freely and showed no apparent restriction in ambient cage activities. Ictal-like hippocampal EEG discharges and concurrent convulsive behaviors that are characteristics of spontaneous recurrent seizures were reliably recorded in a majority of the monitoring experiments in extendedly kindled but not in control animals. However, 1–2 rotary wires were disconnected from the implanted headset in some animals after continuous recordings for ≥5 days. The key features and main limitations of our recording apparatus are discussed. |
| first_indexed | 2024-12-12T10:41:43Z |
| format | Article |
| id | doaj.art-7faa05100ae6463896df035abf3ec267 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-12-12T10:41:43Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-7faa05100ae6463896df035abf3ec2672022-12-22T00:27:02ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532017-09-011110.3389/fnbeh.2017.00172271328Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in MiceNa-Ryum Bin0Na-Ryum Bin1Hongmei Song2Hongmei Song3Chiping Wu4Marcus Lau5Shuzo Sugita6Shuzo Sugita7James H. Eubanks8James H. Eubanks9James H. Eubanks10James H. Eubanks11Liang Zhang12Liang Zhang13Liang Zhang14Krembil Research Institute, University Health NetworkToronto, ON, CanadaDepartment of Physiology, University of TorontoToronto, ON, CanadaKrembil Research Institute, University Health NetworkToronto, ON, CanadaDepartment of Neurosurgery, The First Hospital of Jilin UniversityJilin, ChinaKrembil Research Institute, University Health NetworkToronto, ON, CanadaKrembil Research Institute, University Health NetworkToronto, ON, CanadaKrembil Research Institute, University Health NetworkToronto, ON, CanadaDepartment of Physiology, University of TorontoToronto, ON, CanadaKrembil Research Institute, University Health NetworkToronto, ON, CanadaDepartment of Physiology, University of TorontoToronto, ON, CanadaDivision of Neurosurgery, Department of Surgery, University of TorontoToronto, ON, CanadaThe Epilepsy Research Program of Ontario Brain InstituteToronto, ON, CanadaKrembil Research Institute, University Health NetworkToronto, ON, CanadaThe Epilepsy Research Program of Ontario Brain InstituteToronto, ON, CanadaDivision of Neurology, Department of Medicine, University of TorontoToronto, ON, CanadaWe describe here a simple, cost-effective apparatus for continuous tethered electroencephalographic (EEG) monitoring of spontaneous recurrent seizures in mice. We used a small, low torque slip ring as an EEG commutator, mounted the slip ring onto a standard mouse cage and connected rotary wires of the slip ring directly to animal's implanted headset. Modifications were made in the cage to allow for a convenient installation of the slip ring and accommodation of animal ambient activity. We tested the apparatus for hippocampal EEG recordings in adult C57 black mice. Spontaneous recurrent seizures were induced using extended hippocampal kindling (≥95 daily stimulation). Control animals underwent similar hippocampal electrode implantations but no stimulations were given. Combined EEG and webcam monitoring were performed for 24 h daily for 5–9 consecutive days. During the monitoring periods, the animals moved and accessed water and food freely and showed no apparent restriction in ambient cage activities. Ictal-like hippocampal EEG discharges and concurrent convulsive behaviors that are characteristics of spontaneous recurrent seizures were reliably recorded in a majority of the monitoring experiments in extendedly kindled but not in control animals. However, 1–2 rotary wires were disconnected from the implanted headset in some animals after continuous recordings for ≥5 days. The key features and main limitations of our recording apparatus are discussed.http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00172/fullconvulsiondischargesEEGepilepsyhippocampuskindling |
| spellingShingle | Na-Ryum Bin Na-Ryum Bin Hongmei Song Hongmei Song Chiping Wu Marcus Lau Shuzo Sugita Shuzo Sugita James H. Eubanks James H. Eubanks James H. Eubanks James H. Eubanks Liang Zhang Liang Zhang Liang Zhang Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice Frontiers in Behavioral Neuroscience convulsion discharges EEG epilepsy hippocampus kindling |
| title | Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice |
| title_full | Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice |
| title_fullStr | Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice |
| title_full_unstemmed | Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice |
| title_short | Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice |
| title_sort | continuous monitoring via tethered electroencephalography of spontaneous recurrent seizures in mice |
| topic | convulsion discharges EEG epilepsy hippocampus kindling |
| url | http://journal.frontiersin.org/article/10.3389/fnbeh.2017.00172/full |
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