In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols
The leaves of Phlomis crinita are traditionally used in Algerian medicine for the treatment of pain and inflammatory conditions. In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated on various in vivo experimental models,...
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| Format: | Article |
| Language: | English |
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TMKarpinski Publisher, Tomasz M. Karpiński
2023-04-01
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| Series: | European Journal of Biological Research |
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| Online Access: | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/665 |
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| author | Hanane Boutennoun Lilia Boussouf Nassima Balli Lila Boulekbache Makhlouf Khodir Madani Khaled Al-Qaoud |
| author_facet | Hanane Boutennoun Lilia Boussouf Nassima Balli Lila Boulekbache Makhlouf Khodir Madani Khaled Al-Qaoud |
| author_sort | Hanane Boutennoun |
| collection | DOAJ |
| description |
The leaves of Phlomis crinita are traditionally used in Algerian medicine for the treatment of pain and inflammatory conditions. In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated on various in vivo experimental models, and the total phenolic compounds and flavonoid contents were determined. The carrageenan-induced paw edema method was used to evaluate the anti-inflammatory activity of the extract in vivo, while the in vivo antioxidant effect was assessed by estimating oxidative stress parameters (MDA, CAT, and SOD). Phytochemical screening revealed the presence of substances with high therapeutic values. In vivo anti-inflammatory studies show that plant extract has a significant and dose-dependent impact on the inhibition of edema formation. The maximum percentage inhibition value was 87.79% after 4 h at a concentration of 500 mg/kg. Moreover, the administration of the extract significantly enhanced the activities of antioxidant enzymes in the livers of mice. It significantly (p ˂ 0.05) increased CAT and SOD activities and significantly (p ˂ 0.05) decreased the MDA level activity, compared to the control inflammatory group. Our findings support that Phlomis crinita can be considered as a promising source of therapeutic bioactive compounds.
DOI: http://dx.doi.org/10.5281/zenodo.7860334
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| first_indexed | 2024-04-09T16:08:44Z |
| format | Article |
| id | doaj.art-7fac5c36f1c14fa8bf4b9ffca106b05d |
| institution | Directory Open Access Journal |
| issn | 2449-8955 |
| language | English |
| last_indexed | 2024-04-09T16:08:44Z |
| publishDate | 2023-04-01 |
| publisher | TMKarpinski Publisher, Tomasz M. Karpiński |
| record_format | Article |
| series | European Journal of Biological Research |
| spelling | doaj.art-7fac5c36f1c14fa8bf4b9ffca106b05d2023-04-24T20:42:09ZengTMKarpinski Publisher, Tomasz M. KarpińskiEuropean Journal of Biological Research2449-89552023-04-01132In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenolsHanane Boutennoun0Lilia Boussouf1Nassima Balli2Lila Boulekbache Makhlouf3Khodir Madani4Khaled Al-Qaoud5Molecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria; Laboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, AlgeriaLaboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria; Applied Microbiology and Food Sciences Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, AlgeriaMolecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria; Environment, Health and Biotechnology Laboratory, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, AlgeriaLaboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, AlgeriaLaboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria; Research Centre in Agro-Food Technologies, Targua Ouzemmour Road, 06000 Bejaia, AlgeriaMolecular Immunoparasitology Laboratory, Biological Sciences Department, Faculty of Sciences, University of Yarmouk, Irbid, Jordan The leaves of Phlomis crinita are traditionally used in Algerian medicine for the treatment of pain and inflammatory conditions. In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated on various in vivo experimental models, and the total phenolic compounds and flavonoid contents were determined. The carrageenan-induced paw edema method was used to evaluate the anti-inflammatory activity of the extract in vivo, while the in vivo antioxidant effect was assessed by estimating oxidative stress parameters (MDA, CAT, and SOD). Phytochemical screening revealed the presence of substances with high therapeutic values. In vivo anti-inflammatory studies show that plant extract has a significant and dose-dependent impact on the inhibition of edema formation. The maximum percentage inhibition value was 87.79% after 4 h at a concentration of 500 mg/kg. Moreover, the administration of the extract significantly enhanced the activities of antioxidant enzymes in the livers of mice. It significantly (p ˂ 0.05) increased CAT and SOD activities and significantly (p ˂ 0.05) decreased the MDA level activity, compared to the control inflammatory group. Our findings support that Phlomis crinita can be considered as a promising source of therapeutic bioactive compounds. DOI: http://dx.doi.org/10.5281/zenodo.7860334 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/665Phlomis crinitaPhenolic contentEdemaStress |
| spellingShingle | Hanane Boutennoun Lilia Boussouf Nassima Balli Lila Boulekbache Makhlouf Khodir Madani Khaled Al-Qaoud In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols European Journal of Biological Research Phlomis crinita Phenolic content Edema Stress |
| title | In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols |
| title_full | In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols |
| title_fullStr | In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols |
| title_full_unstemmed | In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols |
| title_short | In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols |
| title_sort | in vivo assessment of anti inflammatory and antioxidant activities of phlomis crinita polyphenols |
| topic | Phlomis crinita Phenolic content Edema Stress |
| url | http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/665 |
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