Intrasplenic Autotransplantation of Hepatocytes in Dogs
Objective- The liver has a unique location in the body for performing multiple functions such as, metabolism, detoxification and synthesis. There are limitation for liver transplantation due to shortage of available donors and requires for sophisticated technology and support surgical teams. The pur...
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| Format: | Article |
| Language: | English |
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Iranian Veterinary Surgery Association (IVSA)
2006-04-01
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| Series: | Iranian Journal of Veterinary Surgery |
| Online Access: | http://www.ivsajournals.com/article_5110_8adeb534df6a966b9a21ab00fc79fab3.pdf |
| _version_ | 1818985427516784640 |
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| author | Amin Bigham Sadegh Seifollah Dehghani Zahra Shafiei Sarvestani |
| author_facet | Amin Bigham Sadegh Seifollah Dehghani Zahra Shafiei Sarvestani |
| author_sort | Amin Bigham Sadegh |
| collection | DOAJ |
| description | Objective- The liver has a unique location in the body for performing multiple functions such as, metabolism, detoxification and synthesis. There are limitation for liver transplantation due to shortage of available donors and requires for sophisticated technology and support surgical teams. The purpose of this study was to investigate the hepatocyte transplantation into the spleen of animal model such as dog to establish basic condition for further studies.
Design- In vivo experimental Study.
Animals- Five Iranian indigenous mixed breed dogs of both sexes, 15/8±3 months old and 19.6±8.2weighting were used in this study.
Procedures- Ventral midline celiotomy was performed under general aneasthesia and liver sampls were obtained using suction technique by a 50cc syringe and 14 gauge needle. Then obtained samples were injected into the same dog 's spleen directly and also via splenic artery, the celiotomy incision sutured and the animal left to recover. On the 16th postoperative day, under general anaesthesia, the gross appearance of spleen were recorded and photographed and for histopathological evaluation, partial splenectomy was performed. The prepared sections were stained with Hematoxylin Eosin [H& E] and Periodic Acid Schiff[PAS] and studied under light microscope.
Results- The gross evaluations of the removed spleen showed a whitish area at the injection site in all of the 5 transplanted dogs, spleen vessels had been dilated and enlarged in diameter. Microscopically the groups of hepatocytes in the center of lymphoid follicles in the white pulp were noted.
Conclusion and Clinical Relevance- Hepatocyte transplantation could be useful for both supporting an acute liver failure and for surviving as a bridge to liver transplantation in terminal liver failure. |
| first_indexed | 2024-12-20T18:34:44Z |
| format | Article |
| id | doaj.art-7fac7c2109dd436f8d043d2a3a1b4cb9 |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-20T18:34:44Z |
| publishDate | 2006-04-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-7fac7c2109dd436f8d043d2a3a1b4cb92022-12-21T19:29:58ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332006-04-0101131355110Intrasplenic Autotransplantation of Hepatocytes in DogsAmin Bigham Sadegh0Seifollah Dehghani1Zahra Shafiei Sarvestani2Department of Veterinary Surgery, Shiraz University, Shiraz, lran.Department of Veterinary Surgery, Shiraz University, Shiraz, lran.Student of Veterinary Surgery, Shiraz University, Shiraz, lran.Objective- The liver has a unique location in the body for performing multiple functions such as, metabolism, detoxification and synthesis. There are limitation for liver transplantation due to shortage of available donors and requires for sophisticated technology and support surgical teams. The purpose of this study was to investigate the hepatocyte transplantation into the spleen of animal model such as dog to establish basic condition for further studies. Design- In vivo experimental Study. Animals- Five Iranian indigenous mixed breed dogs of both sexes, 15/8±3 months old and 19.6±8.2weighting were used in this study. Procedures- Ventral midline celiotomy was performed under general aneasthesia and liver sampls were obtained using suction technique by a 50cc syringe and 14 gauge needle. Then obtained samples were injected into the same dog 's spleen directly and also via splenic artery, the celiotomy incision sutured and the animal left to recover. On the 16th postoperative day, under general anaesthesia, the gross appearance of spleen were recorded and photographed and for histopathological evaluation, partial splenectomy was performed. The prepared sections were stained with Hematoxylin Eosin [H& E] and Periodic Acid Schiff[PAS] and studied under light microscope. Results- The gross evaluations of the removed spleen showed a whitish area at the injection site in all of the 5 transplanted dogs, spleen vessels had been dilated and enlarged in diameter. Microscopically the groups of hepatocytes in the center of lymphoid follicles in the white pulp were noted. Conclusion and Clinical Relevance- Hepatocyte transplantation could be useful for both supporting an acute liver failure and for surviving as a bridge to liver transplantation in terminal liver failure.http://www.ivsajournals.com/article_5110_8adeb534df6a966b9a21ab00fc79fab3.pdf |
| spellingShingle | Amin Bigham Sadegh Seifollah Dehghani Zahra Shafiei Sarvestani Intrasplenic Autotransplantation of Hepatocytes in Dogs Iranian Journal of Veterinary Surgery |
| title | Intrasplenic Autotransplantation of Hepatocytes in Dogs |
| title_full | Intrasplenic Autotransplantation of Hepatocytes in Dogs |
| title_fullStr | Intrasplenic Autotransplantation of Hepatocytes in Dogs |
| title_full_unstemmed | Intrasplenic Autotransplantation of Hepatocytes in Dogs |
| title_short | Intrasplenic Autotransplantation of Hepatocytes in Dogs |
| title_sort | intrasplenic autotransplantation of hepatocytes in dogs |
| url | http://www.ivsajournals.com/article_5110_8adeb534df6a966b9a21ab00fc79fab3.pdf |
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