Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato
The pectinolytic <i>Dickeya solani</i> bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild <i>Dickeya</i> spp. infection and investigated the application of a mixture of two lytic bacteriophages before...
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/620 |
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| author | Karel Petrzik Josef Vacek Martin Kmoch Denisa Binderová Sára Brázdová Ondřej Lenz Rudolf Ševčík |
| author_facet | Karel Petrzik Josef Vacek Martin Kmoch Denisa Binderová Sára Brázdová Ondřej Lenz Rudolf Ševčík |
| author_sort | Karel Petrzik |
| collection | DOAJ |
| description | The pectinolytic <i>Dickeya solani</i> bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild <i>Dickeya</i> spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5–91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe <i>Dickeya</i> infection had 5–33% greater leaf cover and 4–16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11–42% greater, and tuber yield was 25–31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from <i>D. solani</i>. |
| first_indexed | 2024-03-09T11:00:14Z |
| format | Article |
| id | doaj.art-7fad4eb447074cda8147411301b57c79 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:00:14Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7fad4eb447074cda8147411301b57c792023-12-01T01:21:42ZengMDPI AGMicroorganisms2076-26072023-02-0111362010.3390/microorganisms11030620Field Use of Protective Bacteriophages against Pectinolytic Bacteria of PotatoKarel Petrzik0Josef Vacek1Martin Kmoch2Denisa Binderová3Sára Brázdová4Ondřej Lenz5Rudolf Ševčík6Institute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech RepublicDepartment of Growing Technologies, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicLaboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicLaboratory of Virology, Department of Genetic Resources, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech RepublicInstitute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech RepublicInstitute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech RepublicInstitute of Food Preservation, Faculty of Food and Biochemical Technology, University of Chemistry and Technology (VŠCHT), Technická 3, 166 28 Prague, Czech RepublicThe pectinolytic <i>Dickeya solani</i> bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild <i>Dickeya</i> spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5–91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe <i>Dickeya</i> infection had 5–33% greater leaf cover and 4–16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11–42% greater, and tuber yield was 25–31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from <i>D. solani</i>.https://www.mdpi.com/2076-2607/11/3/620<i>Enterobacteriaceae</i><i>Solanum tuberosum</i><i>Limestonevirus</i>biological control |
| spellingShingle | Karel Petrzik Josef Vacek Martin Kmoch Denisa Binderová Sára Brázdová Ondřej Lenz Rudolf Ševčík Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato Microorganisms <i>Enterobacteriaceae</i> <i>Solanum tuberosum</i> <i>Limestonevirus</i> biological control |
| title | Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato |
| title_full | Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato |
| title_fullStr | Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato |
| title_full_unstemmed | Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato |
| title_short | Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato |
| title_sort | field use of protective bacteriophages against pectinolytic bacteria of potato |
| topic | <i>Enterobacteriaceae</i> <i>Solanum tuberosum</i> <i>Limestonevirus</i> biological control |
| url | https://www.mdpi.com/2076-2607/11/3/620 |
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