Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production
Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A nu...
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fenrg.2018.00133/full |
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| author | Daniel L. Williams Daniel L. Williams Rebecca G. Ong Rebecca G. Ong John E. Mullet John E. Mullet David B. Hodge David B. Hodge |
| author_facet | Daniel L. Williams Daniel L. Williams Rebecca G. Ong Rebecca G. Ong John E. Mullet John E. Mullet David B. Hodge David B. Hodge |
| author_sort | Daniel L. Williams |
| collection | DOAJ |
| description | Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed by mild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., <90°C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (>8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolved to ferment xylose. |
| first_indexed | 2024-12-10T17:49:32Z |
| format | Article |
| id | doaj.art-7faf1d660e6446a9ac41c29dbc1b2f47 |
| institution | Directory Open Access Journal |
| issn | 2296-598X |
| language | English |
| last_indexed | 2024-12-10T17:49:32Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Energy Research |
| spelling | doaj.art-7faf1d660e6446a9ac41c29dbc1b2f472022-12-22T01:39:06ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2019-01-01610.3389/fenrg.2018.00133399701Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar ProductionDaniel L. Williams0Daniel L. Williams1Rebecca G. Ong2Rebecca G. Ong3John E. Mullet4John E. Mullet5David B. Hodge6David B. Hodge7Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI, United StatesDOE Great Lakes Bioenergy Research Center, Michigan State University, Madison, MI, United StatesDOE Great Lakes Bioenergy Research Center, Michigan State University, Madison, MI, United StatesDepartment of Chemical Engineering, Michigan Technological University, Houghton, MI, United StatesDOE Great Lakes Bioenergy Research Center, Michigan State University, Madison, MI, United StatesDepartment of Biochemistry & Biophysics, Texas A&M University, College Station, TX, United StatesDepartment of Chemical & Biological Engineering, Montana State University, Bozeman, MT, United StatesDivision of Chemical Engineering, Luleå University of Technology, Luleå, SwedenSorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i. e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed by mild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., <90°C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (>8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolved to ferment xylose.https://www.frontiersin.org/article/10.3389/fenrg.2018.00133/fullsorghumsucrose extractiondecentralized biorefiningpretreatmentcellulosic biofuels |
| spellingShingle | Daniel L. Williams Daniel L. Williams Rebecca G. Ong Rebecca G. Ong John E. Mullet John E. Mullet David B. Hodge David B. Hodge Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production Frontiers in Energy Research sorghum sucrose extraction decentralized biorefining pretreatment cellulosic biofuels |
| title | Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production |
| title_full | Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production |
| title_fullStr | Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production |
| title_full_unstemmed | Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production |
| title_short | Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production |
| title_sort | integration of pretreatment with simultaneous counter current extraction of energy sorghum for high titer mixed sugar production |
| topic | sorghum sucrose extraction decentralized biorefining pretreatment cellulosic biofuels |
| url | https://www.frontiersin.org/article/10.3389/fenrg.2018.00133/full |
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