Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment
A study was conducted on BALB/c mice infected with <i>Leishmania (Leishmania) amazonensis</i> to analyse the effects of 0.5% miltefosine (MTF) hydrogel treatment on cutaneous leishmaniasis (CL) lesions. The mice were treated for 25 days topically, and lesion sizes, parasite loads, histop...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/91/4/54 |
| _version_ | 1797379431374782464 |
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| author | Laura Fernanda Neira Julio Cesar Mantilla Patricia Escobar |
| author_facet | Laura Fernanda Neira Julio Cesar Mantilla Patricia Escobar |
| author_sort | Laura Fernanda Neira |
| collection | DOAJ |
| description | A study was conducted on BALB/c mice infected with <i>Leishmania (Leishmania) amazonensis</i> to analyse the effects of 0.5% miltefosine (MTF) hydrogel treatment on cutaneous leishmaniasis (CL) lesions. The mice were treated for 25 days topically, and lesion sizes, parasite loads, histopathology, ultrastructure, cytokines including interleukin 4 (IL-4), tumour necrosis factor alfa (TNFα), interferon gamma (IFNγ), IL-10, and vascular endothelial growth factor (VEGF) profiles were evaluated on days 0, 12, 25, and 85. After 12 days of treatment, the lesion sizes and parasite numbers decreased. By day 60 post treatment, there were no lesions and only a few parasites. At day 25, there was a temporary papillomatosis reaction, an increase in mast cells, a few giant cells, and granulomas, and a decrease in diffuse inflammatory infiltrate and parasites. Transmission electron microscopy (TEM) examination showed early ultrastructural changes, including macrophages without parasites and vacuoles containing electrodense material. At the different evaluated times, the cytokine regulation indexes (ICRs) decreased for IL-4, TNFα, and VEGF. According to the study, the 0.5% MTF hydrogel was effective and showed positive results from the early stages of usage. The MTF directly targeted parasites, downregulated the release of IL-4, TNFα, and VEGF, increased mast cell production, and induced granuloma reaction during evaluation periods. |
| first_indexed | 2024-03-08T20:23:08Z |
| format | Article |
| id | doaj.art-7fb16867732b42ee977484d13675c72c |
| institution | Directory Open Access Journal |
| issn | 0036-8709 2218-0532 |
| language | English |
| last_indexed | 2024-03-08T20:23:08Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-7fb16867732b42ee977484d13675c72c2023-12-22T14:39:54ZengMDPI AGScientia Pharmaceutica0036-87092218-05322023-11-019145410.3390/scipharm91040054Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine TreatmentLaura Fernanda Neira0Julio Cesar Mantilla1Patricia Escobar2Centro de Investigación en Enfermedades Tropicales (CINTROP-UIS), Departamento de Ciencias Básicas, Escuela de Medicina, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaPAT-UIS, Departamento de Patología, Escuela de Medicina, Universidad Industrial de Santander, Bucaramanga 68002, ColombiaCentro de Investigación en Enfermedades Tropicales (CINTROP-UIS), Departamento de Ciencias Básicas, Escuela de Medicina, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaA study was conducted on BALB/c mice infected with <i>Leishmania (Leishmania) amazonensis</i> to analyse the effects of 0.5% miltefosine (MTF) hydrogel treatment on cutaneous leishmaniasis (CL) lesions. The mice were treated for 25 days topically, and lesion sizes, parasite loads, histopathology, ultrastructure, cytokines including interleukin 4 (IL-4), tumour necrosis factor alfa (TNFα), interferon gamma (IFNγ), IL-10, and vascular endothelial growth factor (VEGF) profiles were evaluated on days 0, 12, 25, and 85. After 12 days of treatment, the lesion sizes and parasite numbers decreased. By day 60 post treatment, there were no lesions and only a few parasites. At day 25, there was a temporary papillomatosis reaction, an increase in mast cells, a few giant cells, and granulomas, and a decrease in diffuse inflammatory infiltrate and parasites. Transmission electron microscopy (TEM) examination showed early ultrastructural changes, including macrophages without parasites and vacuoles containing electrodense material. At the different evaluated times, the cytokine regulation indexes (ICRs) decreased for IL-4, TNFα, and VEGF. According to the study, the 0.5% MTF hydrogel was effective and showed positive results from the early stages of usage. The MTF directly targeted parasites, downregulated the release of IL-4, TNFα, and VEGF, increased mast cell production, and induced granuloma reaction during evaluation periods.https://www.mdpi.com/2218-0532/91/4/54cutaneous leishmaniasiscytokineselectron microscopyhistopathological patternstopical miltefosine |
| spellingShingle | Laura Fernanda Neira Julio Cesar Mantilla Patricia Escobar Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment Scientia Pharmaceutica cutaneous leishmaniasis cytokines electron microscopy histopathological patterns topical miltefosine |
| title | Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment |
| title_full | Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment |
| title_fullStr | Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment |
| title_full_unstemmed | Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment |
| title_short | Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment |
| title_sort | monitoring cutaneous leishmaniasis lesions in mice undergoing topical miltefosine treatment |
| topic | cutaneous leishmaniasis cytokines electron microscopy histopathological patterns topical miltefosine |
| url | https://www.mdpi.com/2218-0532/91/4/54 |
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